Stereoselective high-performance liquid chromatography and analytical method characterization of evacetrapib using a brush-type chiral stationary phase: A challenging isomeric separation requiring a unique eluent system.
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| Title: | Stereoselective high-performance liquid chromatography and analytical method characterization of evacetrapib using a brush-type chiral stationary phase: A challenging isomeric separation requiring a unique eluent system. |
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| Authors: | Sharp, V. Scott1 vss@lilly.com, Stafford, John D.2, Forbes, Robert A.2, Gokey, Megan A.1, Cooper, Mary R.1 |
| Source: | Journal of Chromatography A. Oct2014, Vol. 1363, p183-190. 8p. |
| Subjects: | Stereoselective reactions, High performance liquid chromatography, Chiral stationary phases, Separation of enantiomers, Cardiovascular agents, Drug use testing, Drug analysis |
| Abstract: | Using HPLC chiral separation screening, various columns representing the polysaccharide, macrocyclic antibiotic and brush classes were assessed in multiple chromatographic modes for the separation of evacetrapib, a potential cardiovascular drug, from its enantiomer, two diastereomers and several impurities. Screening data consistently pointed to the brush-type Whelk-O 1 chiral column as most promising for this task. A systematic separation optimization process is outlined using the ( S , S ) Whelk-O 1 chiral column, first for the resolution of the isomers, and later including six potential impurities. A relatively complex yet rugged separation system was eventually identified that effectively resolves all compounds within a reasonable analysis time, and should serve as an adequate tool for evacetrapib bulk drug enantiopurity measurement. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 98482919 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Stereoselective high-performance liquid chromatography and analytical method characterization of evacetrapib using a brush-type chiral stationary phase: A challenging isomeric separation requiring a unique eluent system. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Sharp%2C+V%2E+Scott%22">Sharp, V. Scott</searchLink><relatesTo>1</relatesTo><i> vss@lilly.com</i><br /><searchLink fieldCode="AR" term="%22Stafford%2C+John+D%2E%22">Stafford, John D.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Forbes%2C+Robert+A%2E%22">Forbes, Robert A.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Gokey%2C+Megan+A%2E%22">Gokey, Megan A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cooper%2C+Mary+R%2E%22">Cooper, Mary R.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Oct2014, Vol. 1363, p183-190. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Stereoselective+reactions%22">Stereoselective reactions</searchLink><br /><searchLink fieldCode="DE" term="%22High+performance+liquid+chromatography%22">High performance liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Chiral+stationary+phases%22">Chiral stationary phases</searchLink><br /><searchLink fieldCode="DE" term="%22Separation+of+enantiomers%22">Separation of enantiomers</searchLink><br /><searchLink fieldCode="DE" term="%22Cardiovascular+agents%22">Cardiovascular agents</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+use+testing%22">Drug use testing</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+analysis%22">Drug analysis</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Using HPLC chiral separation screening, various columns representing the polysaccharide, macrocyclic antibiotic and brush classes were assessed in multiple chromatographic modes for the separation of evacetrapib, a potential cardiovascular drug, from its enantiomer, two diastereomers and several impurities. Screening data consistently pointed to the brush-type Whelk-O 1 chiral column as most promising for this task. A systematic separation optimization process is outlined using the ( S , S ) Whelk-O 1 chiral column, first for the resolution of the isomers, and later including six potential impurities. A relatively complex yet rugged separation system was eventually identified that effectively resolves all compounds within a reasonable analysis time, and should serve as an adequate tool for evacetrapib bulk drug enantiopurity measurement. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.chroma.2014.03.048 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 183 Subjects: – SubjectFull: Stereoselective reactions Type: general – SubjectFull: High performance liquid chromatography Type: general – SubjectFull: Chiral stationary phases Type: general – SubjectFull: Separation of enantiomers Type: general – SubjectFull: Cardiovascular agents Type: general – SubjectFull: Drug use testing Type: general – SubjectFull: Drug analysis Type: general Titles: – TitleFull: Stereoselective high-performance liquid chromatography and analytical method characterization of evacetrapib using a brush-type chiral stationary phase: A challenging isomeric separation requiring a unique eluent system. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Sharp, V. Scott – PersonEntity: Name: NameFull: Stafford, John D. – PersonEntity: Name: NameFull: Forbes, Robert A. – PersonEntity: Name: NameFull: Gokey, Megan A. – PersonEntity: Name: NameFull: Cooper, Mary R. IsPartOfRelationships: – BibEntity: Dates: – D: 10 M: 10 Text: Oct2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 00219673 Numbering: – Type: volume Value: 1363 Titles: – TitleFull: Journal of Chromatography A Type: main |
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