Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa.
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| Title: | Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa. |
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| Authors: | Soleilhavoup, C.1,2,3,4, Tsikis, G.1,2,3,4, Labas, V.1,2,3,4,5, Harichaux, G.1,2,3,4,5, Kohnke, P.L.1,2,3,4, Dacheux, J.L.1,2,3,4, Guérin, Y.1,2,3,4, Gatti, J.L.1,2,3,4, de Graaf, S.P.6, Druart, X.1,2,3,4 xavier.druart@tours.inra.fr |
| Source: | Journal of Proteomics. Sep2014, Vol. 109, p245-260. 16p. |
| Subjects: | Spermatozoan proteins, Seminal proteins, Proteomics, Epididymis, Gonads, Molecular chaperones, Glycoproteins |
| Abstract: | Seminal plasma is composed of secretions from the epididymis and the accessory sex glands and plays a critical role in the fertilising ability of spermatozoa. In rams, analysis of seminal plasma by GeLC–MS/MS has allowed the identification of more than 700 proteins, including a high abundance of Binder of Sperm family proteins (BSP1, BSP5, SPADH1, SPADH2), the spermadhesin family (bodhesin2), lactoferrin and newly identified proteins like UPF0762 (C6orf58 gene). When spermatogenesis was stopped by scrotal insulation, changes in the proteome profile revealed the sperm origin of 40 seminal proteins, such as glycolysis pathway enzymes, the chaperonin containing TCP1 (CCT) complex and the 26S proteasome complex. Sperm mobility after liquid preservation (24 h in milk at 15 °C) is male dependent and can be correlated to differences in the seminal plasma proteome, detected by spectral counting. The negative association of zinc alpha-2 glycoprotein (ZAG) with semen preservation was confirmed by the use of recombinant human ZAG, which induced an increase in mobility of fresh sperm, but then decreased sperm mobility after 24 h of incubation. Several sperm membrane proteins interacting with the cytoskeleton, glycolysis enzymes and sperm-associated proteins involved in capacitation correlated with better liquid storage and can be considered as seminal biomarkers of sperm preservation. Biological significance Extensive analysis of the ram seminal plasma proteome reveals a complex and diverse protein composition. This composition varies between males with different sperm preservation abilities. Several proteins were shown to originate from the spermatozoa and positively correlate with sperm liquid preservation, indicating that these proteins can be traced as sperm biomarkers within the seminal plasma. The zinc alpha-2 glycoprotein (ZAG) was found to have a biphasic effect on sperm mobility, with a short-term stimulation followed by a long-term exhaustion of sperm mobility after a 24 h preservation period. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Proteomics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 99064109 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Soleilhavoup%2C+C%2E%22">Soleilhavoup, C.</searchLink><relatesTo>1,2,3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Tsikis%2C+G%2E%22">Tsikis, G.</searchLink><relatesTo>1,2,3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Labas%2C+V%2E%22">Labas, V.</searchLink><relatesTo>1,2,3,4,5</relatesTo><br /><searchLink fieldCode="AR" term="%22Harichaux%2C+G%2E%22">Harichaux, G.</searchLink><relatesTo>1,2,3,4,5</relatesTo><br /><searchLink fieldCode="AR" term="%22Kohnke%2C+P%2EL%2E%22">Kohnke, P.L.</searchLink><relatesTo>1,2,3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Dacheux%2C+J%2EL%2E%22">Dacheux, J.L.</searchLink><relatesTo>1,2,3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Guérin%2C+Y%2E%22">Guérin, Y.</searchLink><relatesTo>1,2,3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Gatti%2C+J%2EL%2E%22">Gatti, J.L.</searchLink><relatesTo>1,2,3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22de+Graaf%2C+S%2EP%2E%22">de Graaf, S.P.</searchLink><relatesTo>6</relatesTo><br /><searchLink fieldCode="AR" term="%22Druart%2C+X%2E%22">Druart, X.</searchLink><relatesTo>1,2,3,4</relatesTo><i> xavier.druart@tours.inra.fr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Proteomics%22">Journal of Proteomics</searchLink>. Sep2014, Vol. 109, p245-260. 16p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Spermatozoan+proteins%22">Spermatozoan proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Seminal+proteins%22">Seminal proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Proteomics%22">Proteomics</searchLink><br /><searchLink fieldCode="DE" term="%22Epididymis%22">Epididymis</searchLink><br /><searchLink fieldCode="DE" term="%22Gonads%22">Gonads</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+chaperones%22">Molecular chaperones</searchLink><br /><searchLink fieldCode="DE" term="%22Glycoproteins%22">Glycoproteins</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Seminal plasma is composed of secretions from the epididymis and the accessory sex glands and plays a critical role in the fertilising ability of spermatozoa. In rams, analysis of seminal plasma by GeLC–MS/MS has allowed the identification of more than 700 proteins, including a high abundance of Binder of Sperm family proteins (BSP1, BSP5, SPADH1, SPADH2), the spermadhesin family (bodhesin2), lactoferrin and newly identified proteins like UPF0762 (C6orf58 gene). When spermatogenesis was stopped by scrotal insulation, changes in the proteome profile revealed the sperm origin of 40 seminal proteins, such as glycolysis pathway enzymes, the chaperonin containing TCP1 (CCT) complex and the 26S proteasome complex. Sperm mobility after liquid preservation (24 h in milk at 15 °C) is male dependent and can be correlated to differences in the seminal plasma proteome, detected by spectral counting. The negative association of zinc alpha-2 glycoprotein (ZAG) with semen preservation was confirmed by the use of recombinant human ZAG, which induced an increase in mobility of fresh sperm, but then decreased sperm mobility after 24 h of incubation. Several sperm membrane proteins interacting with the cytoskeleton, glycolysis enzymes and sperm-associated proteins involved in capacitation correlated with better liquid storage and can be considered as seminal biomarkers of sperm preservation. Biological significance Extensive analysis of the ram seminal plasma proteome reveals a complex and diverse protein composition. This composition varies between males with different sperm preservation abilities. Several proteins were shown to originate from the spermatozoa and positively correlate with sperm liquid preservation, indicating that these proteins can be traced as sperm biomarkers within the seminal plasma. The zinc alpha-2 glycoprotein (ZAG) was found to have a biphasic effect on sperm mobility, with a short-term stimulation followed by a long-term exhaustion of sperm mobility after a 24 h preservation period. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Proteomics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jprot.2014.07.007 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 16 StartPage: 245 Subjects: – SubjectFull: Spermatozoan proteins Type: general – SubjectFull: Seminal proteins Type: general – SubjectFull: Proteomics Type: general – SubjectFull: Epididymis Type: general – SubjectFull: Gonads Type: general – SubjectFull: Molecular chaperones Type: general – SubjectFull: Glycoproteins Type: general Titles: – TitleFull: Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Soleilhavoup, C. – PersonEntity: Name: NameFull: Tsikis, G. – PersonEntity: Name: NameFull: Labas, V. – PersonEntity: Name: NameFull: Harichaux, G. – PersonEntity: Name: NameFull: Kohnke, P.L. – PersonEntity: Name: NameFull: Dacheux, J.L. – PersonEntity: Name: NameFull: Guérin, Y. – PersonEntity: Name: NameFull: Gatti, J.L. – PersonEntity: Name: NameFull: de Graaf, S.P. – PersonEntity: Name: NameFull: Druart, X. IsPartOfRelationships: – BibEntity: Dates: – D: 23 M: 09 Text: Sep2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 18743919 Numbering: – Type: volume Value: 109 Titles: – TitleFull: Journal of Proteomics Type: main |
| ResultId | 1 |
