Dynamics of Lipid Biosynthesis and Redistribution in the Marine Diatom Phaeodactylum tricornutum Under Nitrate Deprivation.
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| Title: | Dynamics of Lipid Biosynthesis and Redistribution in the Marine Diatom Phaeodactylum tricornutum Under Nitrate Deprivation. |
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| Authors: | Burrows, Elizabeth1, Bennette, Nicholas1, Carrieri, Damian2, Dixon, Joseph, Brinker, Anita, Frada, Miguel3, Baldassano, Steven2, Falkowski, Paul, Dismukes, G.1 dismukes@rci.rutgers.edu |
| Source: | BioEnergy Research. Dec2012, Vol. 5 Issue 4, p876-885. 10p. |
| Subjects: | Lipids, Biosynthesis, Biomass energy, Diatoms, Phaeodactylum tricornutum, Lecithin |
| Abstract: | One approach to achieve continuous overproduction of lipids in microalgal 'cell factories' relies upon depletion or removal of nutrients that act as competing electron sinks (e.g., nitrate and sulfate). However, this strategy can only be effective for bioenergy applications if lipid is synthesized primarily de novo (from CO fixation) rather than from the breakdown and interconversion of essential cellular components. In the marine diatom, Phaeodactylum tricornutum, it was determined, using C-bicarbonate, that cell growth in nitrate (NO)-deprived cultures resulted predominantly in de novo lipid synthesis (60 % over 3 days), and this new lipid consisted primarily of triacylglycerides (TAGs). Nearly complete preservation of C occurred in all previously existing TAGs in NO-deprived cultures and thus, further TAG accumulation would not be expected from inhibition of TAG lipolysis. In contrast, both high turnover and depletion of membrane lipids, phosphatidylcholines (PCs), were observed in NO-deprived cultures (both the headgroups and fatty acid chains), while less turnover was observed in NO replete cultures. Liquid chromatography-tandem mass spectrometry mass spectra and C labeling patterns of PC headgroups provided insight into lipid synthesis in marine diatoms, including suggestion of an internal pool of glycine betaine that feeds choline synthesis. It was also observed that 16C fatty acid chains incorporated into TAGs and PCs contained an average of 14 C carbons, indicating substantial incorporation of C-bicarbonate into fatty acid chains under both nutrient states. [ABSTRACT FROM AUTHOR] |
| Copyright of BioEnergy Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 99369107 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s12155-012-9201-7 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 876 Subjects: – SubjectFull: Lipids Type: general – SubjectFull: Biosynthesis Type: general – SubjectFull: Biomass energy Type: general – SubjectFull: Diatoms Type: general – SubjectFull: Phaeodactylum tricornutum Type: general – SubjectFull: Lecithin Type: general Titles: – TitleFull: Dynamics of Lipid Biosynthesis and Redistribution in the Marine Diatom Phaeodactylum tricornutum Under Nitrate Deprivation. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Burrows, Elizabeth – PersonEntity: Name: NameFull: Bennette, Nicholas – PersonEntity: Name: NameFull: Carrieri, Damian – PersonEntity: Name: NameFull: Dixon, Joseph – PersonEntity: Name: NameFull: Brinker, Anita – PersonEntity: Name: NameFull: Frada, Miguel – PersonEntity: Name: NameFull: Baldassano, Steven – PersonEntity: Name: NameFull: Falkowski, Paul – PersonEntity: Name: NameFull: Dismukes, G. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2012 Type: published Y: 2012 Identifiers: – Type: issn-print Value: 19391234 Numbering: – Type: volume Value: 5 – Type: issue Value: 4 Titles: – TitleFull: BioEnergy Research Type: main |
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