View in EDS

Investigation of microbial community structure of a shallow lake after one season copper sulfate algaecide treatment.

Saved in:
Bibliographic Details
Title: Investigation of microbial community structure of a shallow lake after one season copper sulfate algaecide treatment.
Authors: Song, Li-Yan1,2 songliyan@cigit.ac.cn, Wang, Yang-Qing1
Source: Microbiological Research. Jan2015, Vol. 170, p105-113. 9p.
Subjects: Lake microbiology, Copper sulfate, Microorganism populations, Microorganism phylogeny, Algae physiology, Restriction fragment length polymorphisms, Pyrosequencing
Abstract: In present work we described, for the first time, the phylogenic structure of the microbial community in a shallow freshwater lake (Hawk Island Lake, located in the Lower Peninsula of the State of Michigan, U.S.A.) after one season (four times during May to August 2007) of CuSO 4 treatment for algae growth control. The microbial community structure was characterized by terminal restriction fragment length polymorphism (TRFLP), clone library and 454 pyrosequencing. The similar structure of water chemistry measured across three sampling sites suggested that the lake was well mixed. The concentration of chlorophyll a (chl-a) and turbidity was low, 3.35 ± 1.62 μg/L and 2.5 ± 1.9 NTU, respectively, implying that photosynthesis was suppressed. TRFLP profiles showed that the lake was dominated by 16 terminal fragments (TFs), accounting for 85.5–92.6% abundance. Analysis of similarity (ANOSIM) showed that the difference in microbial community structure between upper and lower depths of the water column was not significant ( P = 0.101). These results suggested that the microbial community structure within the lake was similar. Clone library and 454 pyrosequencing indicated that the lake was dominated by freshwater phyla, Proteobacteria , Bacteroides , and Actinobacteria . Moreover, the large number of unclassified bacteria (27.4% of total 2090 454 sequences) suggested a complex microbial community structure in the lake. [ABSTRACT FROM AUTHOR]
Copyright of Microbiological Research is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 99831576
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Investigation of microbial community structure of a shallow lake after one season copper sulfate algaecide treatment.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Song%2C+Li-Yan%22">Song, Li-Yan</searchLink><relatesTo>1,2</relatesTo><i> songliyan@cigit.ac.cn</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Yang-Qing%22">Wang, Yang-Qing</searchLink><relatesTo>1</relatesTo>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Microbiological+Research%22">Microbiological Research</searchLink>. Jan2015, Vol. 170, p105-113. 9p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Lake+microbiology%22">Lake microbiology</searchLink><br /><searchLink fieldCode="DE" term="%22Copper+sulfate%22">Copper sulfate</searchLink><br /><searchLink fieldCode="DE" term="%22Microorganism+populations%22">Microorganism populations</searchLink><br /><searchLink fieldCode="DE" term="%22Microorganism+phylogeny%22">Microorganism phylogeny</searchLink><br /><searchLink fieldCode="DE" term="%22Algae+physiology%22">Algae physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Restriction+fragment+length+polymorphisms%22">Restriction fragment length polymorphisms</searchLink><br /><searchLink fieldCode="DE" term="%22Pyrosequencing%22">Pyrosequencing</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: In present work we described, for the first time, the phylogenic structure of the microbial community in a shallow freshwater lake (Hawk Island Lake, located in the Lower Peninsula of the State of Michigan, U.S.A.) after one season (four times during May to August 2007) of CuSO 4 treatment for algae growth control. The microbial community structure was characterized by terminal restriction fragment length polymorphism (TRFLP), clone library and 454 pyrosequencing. The similar structure of water chemistry measured across three sampling sites suggested that the lake was well mixed. The concentration of chlorophyll a (chl-a) and turbidity was low, 3.35 ± 1.62 μg/L and 2.5 ± 1.9 NTU, respectively, implying that photosynthesis was suppressed. TRFLP profiles showed that the lake was dominated by 16 terminal fragments (TFs), accounting for 85.5–92.6% abundance. Analysis of similarity (ANOSIM) showed that the difference in microbial community structure between upper and lower depths of the water column was not significant ( P = 0.101). These results suggested that the microbial community structure within the lake was similar. Clone library and 454 pyrosequencing indicated that the lake was dominated by freshwater phyla, Proteobacteria , Bacteroides , and Actinobacteria . Moreover, the large number of unclassified bacteria (27.4% of total 2090 454 sequences) suggested a complex microbial community structure in the lake. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Microbiological Research is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=99831576
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.micres.2014.08.008
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 9
        StartPage: 105
    Subjects:
      – SubjectFull: Lake microbiology
        Type: general
      – SubjectFull: Copper sulfate
        Type: general
      – SubjectFull: Microorganism populations
        Type: general
      – SubjectFull: Microorganism phylogeny
        Type: general
      – SubjectFull: Algae physiology
        Type: general
      – SubjectFull: Restriction fragment length polymorphisms
        Type: general
      – SubjectFull: Pyrosequencing
        Type: general
    Titles:
      – TitleFull: Investigation of microbial community structure of a shallow lake after one season copper sulfate algaecide treatment.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Song, Li-Yan
      – PersonEntity:
          Name:
            NameFull: Wang, Yang-Qing
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 01
              Text: Jan2015
              Type: published
              Y: 2015
          Identifiers:
            – Type: issn-print
              Value: 09445013
          Numbering:
            – Type: volume
              Value: 170
          Titles:
            – TitleFull: Microbiological Research
              Type: main
ResultId 1