Extraction, Purification, Characterization And Anticancer Activity Of L-Asparaginase From Novel Plant Source: Caryota Urens (Shivjata).
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| Title: | Extraction, Purification, Characterization And Anticancer Activity Of L-Asparaginase From Novel Plant Source: Caryota Urens (Shivjata). |
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| Authors: | G. N., Chauhan1 gayatrichauhan1992@gmail.com, Upadhye, Vijay J.2, R. R., Gadhvi1 |
| Source: | Educational Administration: Theory & Practice. 2024, Vol. 30 Issue 3, p1942-1951. 10p. |
| Abstract: | L-asparaginase was screened for during the current study in several Caryota urens plant sections. One possible source for L-asparaginase synthesis was identified as the unripe fruit with the highest enzyme activity (925U/ml). The discovery of L-asparaginase in Caryota urens is a first. The enzyme was 38% pure, with an 85-fold purification factor, and 3325U/ml of total enzyme activity after purification. It was discovered that the enzyme's kinetic parameters, Km and Vmax, were 13.8 mM and 100µM/ml, respectively. The partly purified enzyme was shown to be a single protein with a molecular weight of 47 kDa by SDS-PAGE electrophoresis. The cytotoxic effects of L-asparaginase against the K562 and PBMC (peripheral blood mononuclear cell) cell line were investigated; the results showed 26% and 96% (IC50 23.03µg/ml) of cytotoxicity, respectively. Future research can examine the effectiveness of L-asparaginase from Caryota urens L., as it exhibits little glutaminase activity and reduced toxicity toward PBMC. [ABSTRACT FROM AUTHOR] |
| Copyright of Educational Administration: Theory & Practice is the property of Educational Administration: Theory & Practice and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Education Research Complete |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: ehh DbLabel: Education Research Complete An: 177832413 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Extraction, Purification, Characterization And Anticancer Activity Of L-Asparaginase From Novel Plant Source: Caryota Urens (Shivjata). – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22G%2E+N%2E%2C+Chauhan%22">G. N., Chauhan</searchLink><relatesTo>1</relatesTo><i> gayatrichauhan1992@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Upadhye%2C+Vijay+J%2E%22">Upadhye, Vijay J.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22R%2E+R%2E%2C+Gadhvi%22">R. R., Gadhvi</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Educational+Administration%3A+Theory+%26+Practice%22">Educational Administration: Theory & Practice</searchLink>. 2024, Vol. 30 Issue 3, p1942-1951. 10p. – Name: Abstract Label: Abstract Group: Ab Data: L-asparaginase was screened for during the current study in several Caryota urens plant sections. One possible source for L-asparaginase synthesis was identified as the unripe fruit with the highest enzyme activity (925U/ml). The discovery of L-asparaginase in Caryota urens is a first. The enzyme was 38% pure, with an 85-fold purification factor, and 3325U/ml of total enzyme activity after purification. It was discovered that the enzyme's kinetic parameters, Km and Vmax, were 13.8 mM and 100µM/ml, respectively. The partly purified enzyme was shown to be a single protein with a molecular weight of 47 kDa by SDS-PAGE electrophoresis. The cytotoxic effects of L-asparaginase against the K562 and PBMC (peripheral blood mononuclear cell) cell line were investigated; the results showed 26% and 96% (IC50 23.03µg/ml) of cytotoxicity, respectively. Future research can examine the effectiveness of L-asparaginase from Caryota urens L., as it exhibits little glutaminase activity and reduced toxicity toward PBMC. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Educational Administration: Theory & Practice is the property of Educational Administration: Theory & Practice and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.53555/kuey.v30i3.1525 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 1942 Titles: – TitleFull: Extraction, Purification, Characterization And Anticancer Activity Of L-Asparaginase From Novel Plant Source: Caryota Urens (Shivjata). Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: G. N., Chauhan – PersonEntity: Name: NameFull: Upadhye, Vijay J. – PersonEntity: Name: NameFull: R. R., Gadhvi IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: 2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 13004832 Numbering: – Type: volume Value: 30 – Type: issue Value: 3 Titles: – TitleFull: Educational Administration: Theory & Practice Type: main |
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