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Antiproliferative and apoptogenic effects of Cassia fistula L. n-hexane fraction against human cervical cancer (HeLa) cells.

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Title: Antiproliferative and apoptogenic effects of Cassia fistula L. n-hexane fraction against human cervical cancer (HeLa) cells.
Authors: Kaur, Sandeep1,2 (AUTHOR), Pandit, Kritika1 (AUTHOR), Chandel, Madhu2 (AUTHOR), Kaur, Satwinderjeet1 (AUTHOR) sjkaur.gndu@gmail.com
Source: Environmental Science & Pollution Research. Sep2020, Vol. 27 Issue 25, p32017-32033. 17p.
Subject Terms: *HeLa cells, *Cervical cancer, *Cassia (Genus), *Apoptotic bodies, *Cancer cell proliferation
Abstract: The current study was performed to evaluate the antiproliferative and apoptosis-inducing potential of n-hexane fraction from Cassia fistula L. (Caesalpinioideae) fruits. The antiproliferative property of the fraction was determined by MTT assay against cancer cell lines including HeLa, MG-63, IMR-32, and PC-3 with GI50 value of 97.69, 155.2, 143, and 160.2 μg/ml respectively. The fraction was further explored for its apoptotic effect using confocal, SEM, and flow cytometry studies in HeLa cells. It was observed that the treatment of fraction revealed fragmentation of DNA, chromatin condensation, membrane blebbing, and formation of apoptotic bodies in a dose-dependent manner. The fraction also showed a remarkable increase in the level of ROS, mitochondrial depolarization and G0/G1 phase cell cycle arrest, and induction in the phosphatidylserine externalization analyzed using Annexin V-FITC/PI double staining assay in HeLa cells. Kaempferol, Ellagic acid, and Epicatechin are the major phytoconstituents present in the fraction as revealed by the HPLC. The treatment of n-hexane fraction showed downregulation in the gene expression of Bcl-2 and upregulation in the expression level of p53, Bad, and caspase-3 genes analyzed using semi-quantitative RT-PCR in HeLa cells. These results suggest that n-hexane fraction from C. fistula inhibited the proliferation of cervical cancer cells efficiently by the induction of apoptosis. [ABSTRACT FROM AUTHOR]
Database: Energy & Power Source
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DbLabel: Energy & Power Source
An: 144857241
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  Label: Title
  Group: Ti
  Data: Antiproliferative and apoptogenic effects of Cassia fistula L. n-hexane fraction against human cervical cancer (HeLa) cells.
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  Data: <searchLink fieldCode="AR" term="%22Kaur%2C+Sandeep%22">Kaur, Sandeep</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pandit%2C+Kritika%22">Pandit, Kritika</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chandel%2C+Madhu%22">Chandel, Madhu</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kaur%2C+Satwinderjeet%22">Kaur, Satwinderjeet</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> sjkaur.gndu@gmail.com</i>
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  Label: Source
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  Data: <searchLink fieldCode="JN" term="%22Environmental+Science+%26+Pollution+Research%22">Environmental Science & Pollution Research</searchLink>. Sep2020, Vol. 27 Issue 25, p32017-32033. 17p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: *<searchLink fieldCode="DE" term="%22HeLa+cells%22">HeLa cells</searchLink><br />*<searchLink fieldCode="DE" term="%22Cervical+cancer%22">Cervical cancer</searchLink><br />*<searchLink fieldCode="DE" term="%22Cassia+%28Genus%29%22">Cassia (Genus)</searchLink><br />*<searchLink fieldCode="DE" term="%22Apoptotic+bodies%22">Apoptotic bodies</searchLink><br />*<searchLink fieldCode="DE" term="%22Cancer+cell+proliferation%22">Cancer cell proliferation</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The current study was performed to evaluate the antiproliferative and apoptosis-inducing potential of n-hexane fraction from Cassia fistula L. (Caesalpinioideae) fruits. The antiproliferative property of the fraction was determined by MTT assay against cancer cell lines including HeLa, MG-63, IMR-32, and PC-3 with GI50 value of 97.69, 155.2, 143, and 160.2 μg/ml respectively. The fraction was further explored for its apoptotic effect using confocal, SEM, and flow cytometry studies in HeLa cells. It was observed that the treatment of fraction revealed fragmentation of DNA, chromatin condensation, membrane blebbing, and formation of apoptotic bodies in a dose-dependent manner. The fraction also showed a remarkable increase in the level of ROS, mitochondrial depolarization and G0/G1 phase cell cycle arrest, and induction in the phosphatidylserine externalization analyzed using Annexin V-FITC/PI double staining assay in HeLa cells. Kaempferol, Ellagic acid, and Epicatechin are the major phytoconstituents present in the fraction as revealed by the HPLC. The treatment of n-hexane fraction showed downregulation in the gene expression of Bcl-2 and upregulation in the expression level of p53, Bad, and caspase-3 genes analyzed using semi-quantitative RT-PCR in HeLa cells. These results suggest that n-hexane fraction from C. fistula inhibited the proliferation of cervical cancer cells efficiently by the induction of apoptosis. [ABSTRACT FROM AUTHOR]
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1007/s11356-020-08916-9
    Languages:
      – Code: eng
        Text: English
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      Pagination:
        PageCount: 17
        StartPage: 32017
    Subjects:
      – SubjectFull: HeLa cells
        Type: general
      – SubjectFull: Cervical cancer
        Type: general
      – SubjectFull: Cassia (Genus)
        Type: general
      – SubjectFull: Apoptotic bodies
        Type: general
      – SubjectFull: Cancer cell proliferation
        Type: general
    Titles:
      – TitleFull: Antiproliferative and apoptogenic effects of Cassia fistula L. n-hexane fraction against human cervical cancer (HeLa) cells.
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            NameFull: Kaur, Sandeep
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            NameFull: Pandit, Kritika
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            NameFull: Chandel, Madhu
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            NameFull: Kaur, Satwinderjeet
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          Dates:
            – D: 01
              M: 09
              Text: Sep2020
              Type: published
              Y: 2020
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              Value: 27
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              Value: 25
          Titles:
            – TitleFull: Environmental Science & Pollution Research
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