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Using qPCR and microscopy to assess the impact of harvesting and weather conditions on the relationship between Alternaria alternata and Alternaria spp. spores in rural and urban atmospheres.

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Title: Using qPCR and microscopy to assess the impact of harvesting and weather conditions on the relationship between Alternaria alternata and Alternaria spp. spores in rural and urban atmospheres.
Authors: Apangu, Godfrey Philliam1,2 (AUTHOR) godfrey.apangu@rothamsted.ac.uk, Frisk, Carl Alexander1,3 (AUTHOR), Adams-Groom, Beverley1 (AUTHOR), Petch, Geoffrey M.1 (AUTHOR), Hanson, Mary1,4 (AUTHOR), Skjøth, Carsten Ambelas1,5 (AUTHOR)
Source: International Journal of Biometeorology. Jun2023, Vol. 67 Issue 6, p1077-1093. 17p.
Subject Terms: *Alternaria alternata, *Weather, *Harvesting, *Spores, *Cyclones, *Rural-urban migration, *Alternaria, *Fungal spores
Geographic Terms: Worcester (Mass.)
Company/Entity: University of Worcester
Abstract: Alternaria is a plant pathogen and human allergen. Alternaria alternata is one of the most abundant fungal spores in the air. The purpose of this study was to examine whether Alternaria spp. spore concentrations can be used to predict the abundance and spatio-temporal pattern of A. alternata spores in the air. This was investigated by testing the hypothesis that A. alternata dominates airborne Alternaria spp. spores and varies spatio-temporally. Secondarily, we aimed at investigating the relationship between airborne Alternaria spp. spores and the DNA profile of A. alternata spores between two proximate (~ 7 km apart) sites. These were examined by sampling Alternaria spp. spores using Burkard 7-day and cyclone samplers for the period 2016–2018 at Worcester and Lakeside campuses of the University of Worcester, UK. Daily Alternaria spp. spores from the Burkard traps were identified using optical microscopy whilst A. alternata from the cyclone samples was detected and quantified using quantitative polymerase chain reaction (qPCR). The results showed that either A. alternata or other Alternaria species spores dominate the airborne Alternaria spore concentrations, generally depending on weather conditions. Furthermore, although Alternaria spp. spore concentrations were similar for the two proximate sites, A. alternata spore concentrations significantly varied for those sites and it is highly likely that the airborne samples contained large amounts of small fragments of A. alternata. Overall, the study shows that there is a higher abundance of airborne Alternaria allergen than reported by aerobiological networks and the majority is likely to be from spore and hyphal fragments. [ABSTRACT FROM AUTHOR]
Database: Energy & Power Source
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Header DbId: enr
DbLabel: Energy & Power Source
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  Label: Title
  Group: Ti
  Data: Using qPCR and microscopy to assess the impact of harvesting and weather conditions on the relationship between Alternaria alternata and Alternaria spp. spores in rural and urban atmospheres.
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  Data: <searchLink fieldCode="AR" term="%22Apangu%2C+Godfrey+Philliam%22">Apangu, Godfrey Philliam</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> godfrey.apangu@rothamsted.ac.uk</i><br /><searchLink fieldCode="AR" term="%22Frisk%2C+Carl+Alexander%22">Frisk, Carl Alexander</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Adams-Groom%2C+Beverley%22">Adams-Groom, Beverley</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Petch%2C+Geoffrey+M%2E%22">Petch, Geoffrey M.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hanson%2C+Mary%22">Hanson, Mary</searchLink><relatesTo>1,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Skjøth%2C+Carsten+Ambelas%22">Skjøth, Carsten Ambelas</searchLink><relatesTo>1,5</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22International+Journal+of+Biometeorology%22">International Journal of Biometeorology</searchLink>. Jun2023, Vol. 67 Issue 6, p1077-1093. 17p.
– Name: Subject
  Label: Subject Terms
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  Data: *<searchLink fieldCode="DE" term="%22Alternaria+alternata%22">Alternaria alternata</searchLink><br />*<searchLink fieldCode="DE" term="%22Weather%22">Weather</searchLink><br />*<searchLink fieldCode="DE" term="%22Harvesting%22">Harvesting</searchLink><br />*<searchLink fieldCode="DE" term="%22Spores%22">Spores</searchLink><br />*<searchLink fieldCode="DE" term="%22Cyclones%22">Cyclones</searchLink><br />*<searchLink fieldCode="DE" term="%22Rural-urban+migration%22">Rural-urban migration</searchLink><br />*<searchLink fieldCode="DE" term="%22Alternaria%22">Alternaria</searchLink><br />*<searchLink fieldCode="DE" term="%22Fungal+spores%22">Fungal spores</searchLink>
– Name: SubjectGeographic
  Label: Geographic Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Worcester+%28Mass%2E%29%22">Worcester (Mass.)</searchLink>
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  Data: <searchLink fieldCode="DE" term="%22University+of+Worcester%22">University of Worcester</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Alternaria is a plant pathogen and human allergen. Alternaria alternata is one of the most abundant fungal spores in the air. The purpose of this study was to examine whether Alternaria spp. spore concentrations can be used to predict the abundance and spatio-temporal pattern of A. alternata spores in the air. This was investigated by testing the hypothesis that A. alternata dominates airborne Alternaria spp. spores and varies spatio-temporally. Secondarily, we aimed at investigating the relationship between airborne Alternaria spp. spores and the DNA profile of A. alternata spores between two proximate (~ 7 km apart) sites. These were examined by sampling Alternaria spp. spores using Burkard 7-day and cyclone samplers for the period 2016–2018 at Worcester and Lakeside campuses of the University of Worcester, UK. Daily Alternaria spp. spores from the Burkard traps were identified using optical microscopy whilst A. alternata from the cyclone samples was detected and quantified using quantitative polymerase chain reaction (qPCR). The results showed that either A. alternata or other Alternaria species spores dominate the airborne Alternaria spore concentrations, generally depending on weather conditions. Furthermore, although Alternaria spp. spore concentrations were similar for the two proximate sites, A. alternata spore concentrations significantly varied for those sites and it is highly likely that the airborne samples contained large amounts of small fragments of A. alternata. Overall, the study shows that there is a higher abundance of airborne Alternaria allergen than reported by aerobiological networks and the majority is likely to be from spore and hyphal fragments. [ABSTRACT FROM AUTHOR]
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1007/s00484-023-02480-w
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      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 17
        StartPage: 1077
    Subjects:
      – SubjectFull: Alternaria alternata
        Type: general
      – SubjectFull: Weather
        Type: general
      – SubjectFull: Harvesting
        Type: general
      – SubjectFull: Spores
        Type: general
      – SubjectFull: Cyclones
        Type: general
      – SubjectFull: Rural-urban migration
        Type: general
      – SubjectFull: Alternaria
        Type: general
      – SubjectFull: Fungal spores
        Type: general
      – SubjectFull: Worcester (Mass.)
        Type: general
      – SubjectFull: University of Worcester
        Type: general
    Titles:
      – TitleFull: Using qPCR and microscopy to assess the impact of harvesting and weather conditions on the relationship between Alternaria alternata and Alternaria spp. spores in rural and urban atmospheres.
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            NameFull: Apangu, Godfrey Philliam
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            NameFull: Frisk, Carl Alexander
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            NameFull: Adams-Groom, Beverley
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            NameFull: Petch, Geoffrey M.
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            NameFull: Hanson, Mary
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            NameFull: Skjøth, Carsten Ambelas
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          Dates:
            – D: 01
              M: 06
              Text: Jun2023
              Type: published
              Y: 2023
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            – Type: issn-print
              Value: 00207128
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              Value: 67
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            – TitleFull: International Journal of Biometeorology
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