Impact of Hydraulic Retention Time on a Novel Single-Chamber Aerobic Granular Sludge Continuous-Flow Reactor Treating Wastewater: Physicochemical and Microbial Characterisation.

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Title: Impact of Hydraulic Retention Time on a Novel Single-Chamber Aerobic Granular Sludge Continuous-Flow Reactor Treating Wastewater: Physicochemical and Microbial Characterisation.
Authors: Rosa-Masegosa, Aurora1,2 (AUTHOR) aurorarm@ugr.es, Gonzalez-Lopez, Jesus1,2 (AUTHOR) jgl@ugr.es, Gonzalez-Martinez, Alejandro1,2 (AUTHOR) agon@ugr.es, Muñoz-Palazon, Barbara1 (AUTHOR) bmp@ugr.es
Source: Water, Air & Soil Pollution. Mar2026, Vol. 237 Issue 6, p1-23. 23p.
Subject Terms: *Continuous flow reactors, *Wastewater treatment, *Microbiology, *Organic compounds removal (Sewage purification)
Abstract: In the frame of aerobic granular sludge systems, finding a simple and efficient continuous-flow reactor is crucial to reduce costs and enhance the treatment efficiency. An optimal continuous-flow design must be able to operate at different hydraulic retention times (HRTs) to ensure effluent quality, maintaining the granular stability. This research evaluates the effect of HRT on operational parameters, performance, biomass characteristics and microbial dynamics of a novel single-chamber continuous-flow reactor for aerobic granular sludge technology to assess the feasibility of this compact design for full-scale implementation. For that, four bioreactors were operated under 8, 6, 4 and 2 h of HRT. Results revealed that the bioreactor operated at 2 h of HRT reached around 60% of organic matter removal. Bioreactors operated at 8, 6 and 4 h of HRT achieved stable granules and a higher removal performance of organic matter (close to 90% of biological oxygen demand at day 5). However, the most environmentally effective HRT was 4 h, featuring the optimal granular stability, allowing the treatment of larger volume of water in shorter time. Microbial communities were modulated according to the operational conditions. Hypocreales, Spirosomaceae and Brevundimonas were ubiquitous in all bioreactors. The family Chitinophagaceae formed the microbial core of granules from bioreactors with optimal performance. The bioreactor operated at the shortest HRT exhibited the highest changes in microbial dynamics. This study revealed the competitiveness of the novel design due to its performance and granular stability maintenance under different HRTs. Highlights: Novel AGS-CFR achieved high organic removal results at 8, 6 and 4 h of HRT. Two hours of HRT is not enough to reach optimal macro pollutants removal rates. Hydraulic retention times from 8 to 4 h maintained granular integrity. Microbial community was regulated according to the HRT. Spirosomaceae, Brevundimonas and Hypocreales were ubiquitous in all bioreactors. [ABSTRACT FROM AUTHOR]
Database: Energy & Power Source
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Header DbId: enr
DbLabel: Energy & Power Source
An: 191451519
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Impact of Hydraulic Retention Time on a Novel Single-Chamber Aerobic Granular Sludge Continuous-Flow Reactor Treating Wastewater: Physicochemical and Microbial Characterisation.
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  Data: <searchLink fieldCode="AR" term="%22Rosa-Masegosa%2C+Aurora%22">Rosa-Masegosa, Aurora</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> aurorarm@ugr.es</i><br /><searchLink fieldCode="AR" term="%22Gonzalez-Lopez%2C+Jesus%22">Gonzalez-Lopez, Jesus</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> jgl@ugr.es</i><br /><searchLink fieldCode="AR" term="%22Gonzalez-Martinez%2C+Alejandro%22">Gonzalez-Martinez, Alejandro</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> agon@ugr.es</i><br /><searchLink fieldCode="AR" term="%22Muñoz-Palazon%2C+Barbara%22">Muñoz-Palazon, Barbara</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> bmp@ugr.es</i>
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  Data: <searchLink fieldCode="JN" term="%22Water%2C+Air+%26+Soil+Pollution%22">Water, Air & Soil Pollution</searchLink>. Mar2026, Vol. 237 Issue 6, p1-23. 23p.
– Name: Subject
  Label: Subject Terms
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  Data: *<searchLink fieldCode="DE" term="%22Continuous+flow+reactors%22">Continuous flow reactors</searchLink><br />*<searchLink fieldCode="DE" term="%22Wastewater+treatment%22">Wastewater treatment</searchLink><br />*<searchLink fieldCode="DE" term="%22Microbiology%22">Microbiology</searchLink><br />*<searchLink fieldCode="DE" term="%22Organic+compounds+removal+%28Sewage+purification%29%22">Organic compounds removal (Sewage purification)</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: In the frame of aerobic granular sludge systems, finding a simple and efficient continuous-flow reactor is crucial to reduce costs and enhance the treatment efficiency. An optimal continuous-flow design must be able to operate at different hydraulic retention times (HRTs) to ensure effluent quality, maintaining the granular stability. This research evaluates the effect of HRT on operational parameters, performance, biomass characteristics and microbial dynamics of a novel single-chamber continuous-flow reactor for aerobic granular sludge technology to assess the feasibility of this compact design for full-scale implementation. For that, four bioreactors were operated under 8, 6, 4 and 2 h of HRT. Results revealed that the bioreactor operated at 2 h of HRT reached around 60% of organic matter removal. Bioreactors operated at 8, 6 and 4 h of HRT achieved stable granules and a higher removal performance of organic matter (close to 90% of biological oxygen demand at day 5). However, the most environmentally effective HRT was 4 h, featuring the optimal granular stability, allowing the treatment of larger volume of water in shorter time. Microbial communities were modulated according to the operational conditions. Hypocreales, Spirosomaceae and Brevundimonas were ubiquitous in all bioreactors. The family Chitinophagaceae formed the microbial core of granules from bioreactors with optimal performance. The bioreactor operated at the shortest HRT exhibited the highest changes in microbial dynamics. This study revealed the competitiveness of the novel design due to its performance and granular stability maintenance under different HRTs. Highlights: Novel AGS-CFR achieved high organic removal results at 8, 6 and 4 h of HRT. Two hours of HRT is not enough to reach optimal macro pollutants removal rates. Hydraulic retention times from 8 to 4 h maintained granular integrity. Microbial community was regulated according to the HRT. Spirosomaceae, Brevundimonas and Hypocreales were ubiquitous in all bioreactors. [ABSTRACT FROM AUTHOR]
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RecordInfo BibRecord:
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        Value: 10.1007/s11270-025-09037-0
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      – Code: eng
        Text: English
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        PageCount: 23
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    Subjects:
      – SubjectFull: Continuous flow reactors
        Type: general
      – SubjectFull: Wastewater treatment
        Type: general
      – SubjectFull: Microbiology
        Type: general
      – SubjectFull: Organic compounds removal (Sewage purification)
        Type: general
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      – TitleFull: Impact of Hydraulic Retention Time on a Novel Single-Chamber Aerobic Granular Sludge Continuous-Flow Reactor Treating Wastewater: Physicochemical and Microbial Characterisation.
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            NameFull: Rosa-Masegosa, Aurora
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            NameFull: Gonzalez-Lopez, Jesus
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            NameFull: Gonzalez-Martinez, Alejandro
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            NameFull: Muñoz-Palazon, Barbara
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            – D: 15
              M: 03
              Text: Mar2026
              Type: published
              Y: 2026
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