View in EDS

A Course of Hands-On Nanopore Experiments for Undergraduates: Single-Molecule Detection with Portable Electrochemical Instruments

Saved in:
Bibliographic Details
Title: A Course of Hands-On Nanopore Experiments for Undergraduates: Single-Molecule Detection with Portable Electrochemical Instruments
Language: English
Authors: Hu, Zheng-Li, Ying, Yi-Lun (ORCID 0000-0001-6217-256X), Huo, Ming-Zhu, Kong, Xuan-Feng, Yu, Xiao-Dong, Zhang, Jian-Rong, Long, Yi-Tao (ORCID 0000-0003-2571-7457)
Source: Journal of Chemical Education. Dec 2020 97(12):4345-4354.
Availability: Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Peer Reviewed: Y
Page Count: 10
Publication Date: 2020
Document Type: Journal Articles
Reports - Descriptive
Education Level: Higher Education
Postsecondary Education
Descriptors: College Science, Undergraduate Study, Science Instruction, Hands on Science, Science Experiments, Molecular Structure, Chemistry, Science Laboratories, Laboratory Experiments
DOI: 10.1021/acs.jchemed.0c00389
ISSN: 0021-9584
Abstract: The prompt introduction of single-molecule science into undergraduates' curricula is an urgent but challenging task. However, the research experiments are often too complicated and the instruments are too expensive to be widely transferred into a compulsory course of undergraduate students. Herein, we introduce a recently developed nanopore technique into an accessible laboratory course for students with the advantages of simple operation, high repeatability, and low cost. The nanopore course serves to help undergraduates in practice to understand single-molecule behaviors, think at a single molecule level, and gradually develop single-molecule solutions for scientific issues. The learning activities for undergraduate students include setup of the instrument, fabrication of a biological nanopore on an artificial lipid bilayer membrane, detection of individual oligonucleotides, and data analysis. An experiment example is single-molecule detection of the model DNA sample poly(dA)[subscript n] (n = 4, 5) using the aerolysin nanopore. As our developed nanopore sensing system is simple, portable, and easy to handle, the experiment course could be implemented and popularized in undergraduates' laboratory education. For the undergraduate students who have taken general chemistry and/or biochemistry courses, this hands-on experience of nanopore experiments would benefit them with a better understanding of the chemical world at a single-molecule level and further using single-molecule tools in various applications including DNA and/or protein sequencing.
Abstractor: As Provided
Entry Date: 2021
Accession Number: EJ1279875
Database: ERIC
FullText Text:
  Availability: 0
Header DbId: eric
DbLabel: ERIC
An: EJ1279875
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: A Course of Hands-On Nanopore Experiments for Undergraduates: Single-Molecule Detection with Portable Electrochemical Instruments
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Hu%2C+Zheng-Li%22">Hu, Zheng-Li</searchLink><br /><searchLink fieldCode="AR" term="%22Ying%2C+Yi-Lun%22">Ying, Yi-Lun</searchLink> (ORCID <externalLink term="http://orcid.org/0000-0001-6217-256X">0000-0001-6217-256X</externalLink>)<br /><searchLink fieldCode="AR" term="%22Huo%2C+Ming-Zhu%22">Huo, Ming-Zhu</searchLink><br /><searchLink fieldCode="AR" term="%22Kong%2C+Xuan-Feng%22">Kong, Xuan-Feng</searchLink><br /><searchLink fieldCode="AR" term="%22Yu%2C+Xiao-Dong%22">Yu, Xiao-Dong</searchLink><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Jian-Rong%22">Zhang, Jian-Rong</searchLink><br /><searchLink fieldCode="AR" term="%22Long%2C+Yi-Tao%22">Long, Yi-Tao</searchLink> (ORCID <externalLink term="http://orcid.org/0000-0003-2571-7457">0000-0003-2571-7457</externalLink>)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="SO" term="%22Journal+of+Chemical+Education%22"><i>Journal of Chemical Education</i></searchLink>. Dec 2020 97(12):4345-4354.
– Name: Avail
  Label: Availability
  Group: Avail
  Data: Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
– Name: PeerReviewed
  Label: Peer Reviewed
  Group: SrcInfo
  Data: Y
– Name: Pages
  Label: Page Count
  Group: Src
  Data: 10
– Name: DatePubCY
  Label: Publication Date
  Group: Date
  Data: 2020
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: Journal Articles<br />Reports - Descriptive
– Name: Audience
  Label: Education Level
  Group: Audnce
  Data: <searchLink fieldCode="EL" term="%22Higher+Education%22">Higher Education</searchLink><br /><searchLink fieldCode="EL" term="%22Postsecondary+Education%22">Postsecondary Education</searchLink>
– Name: Subject
  Label: Descriptors
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22College+Science%22">College Science</searchLink><br /><searchLink fieldCode="DE" term="%22Undergraduate+Study%22">Undergraduate Study</searchLink><br /><searchLink fieldCode="DE" term="%22Science+Instruction%22">Science Instruction</searchLink><br /><searchLink fieldCode="DE" term="%22Hands+on+Science%22">Hands on Science</searchLink><br /><searchLink fieldCode="DE" term="%22Science+Experiments%22">Science Experiments</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+Structure%22">Molecular Structure</searchLink><br /><searchLink fieldCode="DE" term="%22Chemistry%22">Chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Science+Laboratories%22">Science Laboratories</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+Experiments%22">Laboratory Experiments</searchLink>
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1021/acs.jchemed.0c00389
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 0021-9584
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The prompt introduction of single-molecule science into undergraduates' curricula is an urgent but challenging task. However, the research experiments are often too complicated and the instruments are too expensive to be widely transferred into a compulsory course of undergraduate students. Herein, we introduce a recently developed nanopore technique into an accessible laboratory course for students with the advantages of simple operation, high repeatability, and low cost. The nanopore course serves to help undergraduates in practice to understand single-molecule behaviors, think at a single molecule level, and gradually develop single-molecule solutions for scientific issues. The learning activities for undergraduate students include setup of the instrument, fabrication of a biological nanopore on an artificial lipid bilayer membrane, detection of individual oligonucleotides, and data analysis. An experiment example is single-molecule detection of the model DNA sample poly(dA)[subscript n] (n = 4, 5) using the aerolysin nanopore. As our developed nanopore sensing system is simple, portable, and easy to handle, the experiment course could be implemented and popularized in undergraduates' laboratory education. For the undergraduate students who have taken general chemistry and/or biochemistry courses, this hands-on experience of nanopore experiments would benefit them with a better understanding of the chemical world at a single-molecule level and further using single-molecule tools in various applications including DNA and/or protein sequencing.
– Name: AbstractInfo
  Label: Abstractor
  Group: Ab
  Data: As Provided
– Name: DateEntry
  Label: Entry Date
  Group: Date
  Data: 2021
– Name: AN
  Label: Accession Number
  Group: ID
  Data: EJ1279875
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=eric&AN=EJ1279875
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1021/acs.jchemed.0c00389
    Languages:
      – Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 4345
    Subjects:
      – SubjectFull: College Science
        Type: general
      – SubjectFull: Undergraduate Study
        Type: general
      – SubjectFull: Science Instruction
        Type: general
      – SubjectFull: Hands on Science
        Type: general
      – SubjectFull: Science Experiments
        Type: general
      – SubjectFull: Molecular Structure
        Type: general
      – SubjectFull: Chemistry
        Type: general
      – SubjectFull: Science Laboratories
        Type: general
      – SubjectFull: Laboratory Experiments
        Type: general
    Titles:
      – TitleFull: A Course of Hands-On Nanopore Experiments for Undergraduates: Single-Molecule Detection with Portable Electrochemical Instruments
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Hu, Zheng-Li
      – PersonEntity:
          Name:
            NameFull: Ying, Yi-Lun
      – PersonEntity:
          Name:
            NameFull: Huo, Ming-Zhu
      – PersonEntity:
          Name:
            NameFull: Kong, Xuan-Feng
      – PersonEntity:
          Name:
            NameFull: Yu, Xiao-Dong
      – PersonEntity:
          Name:
            NameFull: Zhang, Jian-Rong
      – PersonEntity:
          Name:
            NameFull: Long, Yi-Tao
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 12
              Type: published
              Y: 2020
          Identifiers:
            – Type: issn-print
              Value: 0021-9584
          Numbering:
            – Type: volume
              Value: 97
            – Type: issue
              Value: 12
          Titles:
            – TitleFull: Journal of Chemical Education
              Type: main
ResultId 1