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Manipulative-Based Activity Using Pop Beads for Demonstration of Sanger Sequencing

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Title: Manipulative-Based Activity Using Pop Beads for Demonstration of Sanger Sequencing
Authors: Kylie Light, Jordan Brooks, Yun-Seok Choi (ORCID 0000-0002-2709-8306)
Source: Journal of Chemical Education. 2023 100(8):3138-3143.
Availability: Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Peer Reviewed: Y
Page Count: 6
Publication Date: 2023
Sponsoring Agency: National Institute of General Medical Sciences (NIGMS) (DHHS/NIH)
Contract Number: P20GM103443
Intended Audience: Teachers
Document Type: Journal Articles
Reports - Descriptive
Descriptors: Genetics, Biochemistry, Science Instruction, Science Activities, Manipulative Materials, Visual Aids, Kinesthetic Methods, Demonstrations (Educational)
DOI: 10.1021/acs.jchemed.3c00177
ISSN: 0021-9584
1938-1328
Abstract: Sanger sequencing, also known as dideoxy sequencing, is a widely used method for DNA sequencing, particularly for cloned plasmids and clinical samples. This technique requires a combination of essential biochemistry skills, such as a chain-termination reaction, gel electrophoresis, and fluorescence detection. Unfortunately, there is a lack of activities that replicate the Sanger sequencing process for students to learn and practice these skills. To address this issue, a manipulative-based Sanger sequencing activity was developed that incorporates colorful pop beads to demonstrate a chain-termination reaction, separation of products, and fluorescence detection. The beads represent deoxynucleotides and dideoxynucleotides, allowing for a visual representation of DNA fragment generation. This kinesthetic learning activity offers a high visual impact for students, aiding in their understanding of the Sanger sequencing process, and can also be used to illustrate polymerase chain reaction (PCR)-based techniques.
Abstractor: As Provided
Entry Date: 2024
Accession Number: EJ1444832
Database: ERIC
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://eric.ed.gov/contentdelivery/servlet/ERICServlet?accno=EJ1444832
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  Data: Manipulative-Based Activity Using Pop Beads for Demonstration of Sanger Sequencing
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  Data: <searchLink fieldCode="AR" term="%22Kylie+Light%22">Kylie Light</searchLink><br /><searchLink fieldCode="AR" term="%22Jordan+Brooks%22">Jordan Brooks</searchLink><br /><searchLink fieldCode="AR" term="%22Yun-Seok+Choi%22">Yun-Seok Choi</searchLink> (ORCID <externalLink term="https://orcid.org/0000-0002-2709-8306">0000-0002-2709-8306</externalLink>)
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  Data: <searchLink fieldCode="SO" term="%22Journal+of+Chemical+Education%22"><i>Journal of Chemical Education</i></searchLink>. 2023 100(8):3138-3143.
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  Data: Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
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  Data: Y
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  Data: 6
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  Label: Publication Date
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  Data: 2023
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  Data: National Institute of General Medical Sciences (NIGMS) (DHHS/NIH)
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  Data: Journal Articles<br />Reports - Descriptive
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  Data: <searchLink fieldCode="DE" term="%22Genetics%22">Genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemistry%22">Biochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Science+Instruction%22">Science Instruction</searchLink><br /><searchLink fieldCode="DE" term="%22Science+Activities%22">Science Activities</searchLink><br /><searchLink fieldCode="DE" term="%22Manipulative+Materials%22">Manipulative Materials</searchLink><br /><searchLink fieldCode="DE" term="%22Visual+Aids%22">Visual Aids</searchLink><br /><searchLink fieldCode="DE" term="%22Kinesthetic+Methods%22">Kinesthetic Methods</searchLink><br /><searchLink fieldCode="DE" term="%22Demonstrations+%28Educational%29%22">Demonstrations (Educational)</searchLink>
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  Data: 10.1021/acs.jchemed.3c00177
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  Data: 0021-9584<br />1938-1328
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Sanger sequencing, also known as dideoxy sequencing, is a widely used method for DNA sequencing, particularly for cloned plasmids and clinical samples. This technique requires a combination of essential biochemistry skills, such as a chain-termination reaction, gel electrophoresis, and fluorescence detection. Unfortunately, there is a lack of activities that replicate the Sanger sequencing process for students to learn and practice these skills. To address this issue, a manipulative-based Sanger sequencing activity was developed that incorporates colorful pop beads to demonstrate a chain-termination reaction, separation of products, and fluorescence detection. The beads represent deoxynucleotides and dideoxynucleotides, allowing for a visual representation of DNA fragment generation. This kinesthetic learning activity offers a high visual impact for students, aiding in their understanding of the Sanger sequencing process, and can also be used to illustrate polymerase chain reaction (PCR)-based techniques.
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  Data: As Provided
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  Data: 2024
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  Label: Accession Number
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  Data: EJ1444832
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      – SubjectFull: Science Activities
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      – SubjectFull: Manipulative Materials
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      – SubjectFull: Visual Aids
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      – TitleFull: Manipulative-Based Activity Using Pop Beads for Demonstration of Sanger Sequencing
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            NameFull: Yun-Seok Choi
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