View in EDS

An Equation-Based Approach to Estimating Enzymatic Activity from Experimental Data

Saved in:
Bibliographic Details
Title: An Equation-Based Approach to Estimating Enzymatic Activity from Experimental Data
Language: English
Authors: Daniel Ojeda-del Sol (ORCID 0000-0003-2315-1170), Anthony Aguiar-Medina (ORCID 0000-0003-1715-4412), Isel Pascual-Alonso (ORCID 0000-0002-6316-9327)
Source: Biochemistry and Molecular Biology Education. 2025 53(5):521-526.
Availability: Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us
Peer Reviewed: Y
Page Count: 6
Publication Date: 2025
Document Type: Journal Articles
Reports - Research
Education Level: Higher Education
Postsecondary Education
Descriptors: Biochemistry, Equations (Mathematics), Data, Science Activities, College Science, Science Instruction, Kinetics, Computation, Undergraduate Students, Biological Sciences
DOI: 10.1002/bmb.70004
ISSN: 1470-8175
1539-3429
Abstract: The study and analysis of enzymatic reactions are essential for characterizing various biological systems. As part of these procedures, it is important to understand how to quantify the biological activity of these proteins in terms of enzymatic activity. This work proposes a general equation for determining enzymatic activity based on experimental data, along with the theoretical elements that support it. A model exercise is included, and the methodology for calculating both enzymatic activity and specific enzymatic activity is outlined in detail, step by step. This procedure is specifically designed for undergraduate students in the biological sciences who are interested in enzyme kinetics.
Abstractor: As Provided
Entry Date: 2025
Accession Number: EJ1483774
Database: ERIC
FullText Text:
  Availability: 0
Header DbId: eric
DbLabel: ERIC
An: EJ1483774
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: An Equation-Based Approach to Estimating Enzymatic Activity from Experimental Data
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Daniel+Ojeda-del+Sol%22">Daniel Ojeda-del Sol</searchLink> (ORCID <externalLink term="https://orcid.org/0000-0003-2315-1170">0000-0003-2315-1170</externalLink>)<br /><searchLink fieldCode="AR" term="%22Anthony+Aguiar-Medina%22">Anthony Aguiar-Medina</searchLink> (ORCID <externalLink term="https://orcid.org/0000-0003-1715-4412">0000-0003-1715-4412</externalLink>)<br /><searchLink fieldCode="AR" term="%22Isel+Pascual-Alonso%22">Isel Pascual-Alonso</searchLink> (ORCID <externalLink term="https://orcid.org/0000-0002-6316-9327">0000-0002-6316-9327</externalLink>)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="SO" term="%22Biochemistry+and+Molecular+Biology+Education%22"><i>Biochemistry and Molecular Biology Education</i></searchLink>. 2025 53(5):521-526.
– Name: Avail
  Label: Availability
  Group: Avail
  Data: Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us
– Name: PeerReviewed
  Label: Peer Reviewed
  Group: SrcInfo
  Data: Y
– Name: Pages
  Label: Page Count
  Group: Src
  Data: 6
– Name: DatePubCY
  Label: Publication Date
  Group: Date
  Data: 2025
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: Journal Articles<br />Reports - Research
– Name: Audience
  Label: Education Level
  Group: Audnce
  Data: <searchLink fieldCode="EL" term="%22Higher+Education%22">Higher Education</searchLink><br /><searchLink fieldCode="EL" term="%22Postsecondary+Education%22">Postsecondary Education</searchLink>
– Name: Subject
  Label: Descriptors
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Biochemistry%22">Biochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Equations+%28Mathematics%29%22">Equations (Mathematics)</searchLink><br /><searchLink fieldCode="DE" term="%22Data%22">Data</searchLink><br /><searchLink fieldCode="DE" term="%22Science+Activities%22">Science Activities</searchLink><br /><searchLink fieldCode="DE" term="%22College+Science%22">College Science</searchLink><br /><searchLink fieldCode="DE" term="%22Science+Instruction%22">Science Instruction</searchLink><br /><searchLink fieldCode="DE" term="%22Kinetics%22">Kinetics</searchLink><br /><searchLink fieldCode="DE" term="%22Computation%22">Computation</searchLink><br /><searchLink fieldCode="DE" term="%22Undergraduate+Students%22">Undergraduate Students</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+Sciences%22">Biological Sciences</searchLink>
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1002/bmb.70004
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 1470-8175<br />1539-3429
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The study and analysis of enzymatic reactions are essential for characterizing various biological systems. As part of these procedures, it is important to understand how to quantify the biological activity of these proteins in terms of enzymatic activity. This work proposes a general equation for determining enzymatic activity based on experimental data, along with the theoretical elements that support it. A model exercise is included, and the methodology for calculating both enzymatic activity and specific enzymatic activity is outlined in detail, step by step. This procedure is specifically designed for undergraduate students in the biological sciences who are interested in enzyme kinetics.
– Name: AbstractInfo
  Label: Abstractor
  Group: Ab
  Data: As Provided
– Name: DateEntry
  Label: Entry Date
  Group: Date
  Data: 2025
– Name: AN
  Label: Accession Number
  Group: ID
  Data: EJ1483774
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=eric&AN=EJ1483774
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1002/bmb.70004
    Languages:
      – Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 6
        StartPage: 521
    Subjects:
      – SubjectFull: Biochemistry
        Type: general
      – SubjectFull: Equations (Mathematics)
        Type: general
      – SubjectFull: Data
        Type: general
      – SubjectFull: Science Activities
        Type: general
      – SubjectFull: College Science
        Type: general
      – SubjectFull: Science Instruction
        Type: general
      – SubjectFull: Kinetics
        Type: general
      – SubjectFull: Computation
        Type: general
      – SubjectFull: Undergraduate Students
        Type: general
      – SubjectFull: Biological Sciences
        Type: general
    Titles:
      – TitleFull: An Equation-Based Approach to Estimating Enzymatic Activity from Experimental Data
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Daniel Ojeda-del Sol
      – PersonEntity:
          Name:
            NameFull: Anthony Aguiar-Medina
      – PersonEntity:
          Name:
            NameFull: Isel Pascual-Alonso
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 09
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 1470-8175
            – Type: issn-electronic
              Value: 1539-3429
          Numbering:
            – Type: volume
              Value: 53
            – Type: issue
              Value: 5
          Titles:
            – TitleFull: Biochemistry and Molecular Biology Education
              Type: main
ResultId 1