Teaching Bio-Layer Interferometry: Protein-Protein versus Protein-Small Molecule Interaction Analysis on an Octet R8
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| Title: | Teaching Bio-Layer Interferometry: Protein-Protein versus Protein-Small Molecule Interaction Analysis on an Octet R8 |
|---|---|
| Language: | English |
| Authors: | Wen Chen (ORCID |
| Source: | Biochemistry and Molecular Biology Education. 2026 54(3):297-307. |
| Availability: | Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us |
| Peer Reviewed: | Y |
| Page Count: | 11 |
| Publication Date: | 2026 |
| Document Type: | Journal Articles Reports - Descriptive |
| Education Level: | Higher Education Postsecondary Education |
| Descriptors: | College Science, Undergraduate Study, Science Activities, Science Instruction, Molecular Biology |
| DOI: | 10.1002/bmb.70049 |
| ISSN: | 1470-8175 1539-3429 |
| Abstract: | We describe a classroom-ready bio-layer interferometry protocol for upper-level undergraduates that contrasts a macromolecular interaction with a small-molecule interaction on an Octet R8. Biotinylated bovine serum albumin (BSA) is immobilized on Super Streptavidin sensors to enable two assays in parallel: (i) binding of a commercially available anti-BSA IgG (protein-protein) and (ii) binding of a long-chain fatty acid such as palmitate (protein-small molecule). The exercise emphasizes assay design decisions that differ by analyte class-sensor chemistry and loading density, solvent matching and reference subtraction, association/dissociation timing, and the choice of data-analysis pathway (global 1:1 kinetic fitting for IgG vs. steady-state isotherms for fatty acids). Representative data yield nanomolar K[subscript D] values for BSA-IgG and micromolar apparent K[subscript D] for BSA-fatty acid, with correspondingly larger response amplitudes and slower off-rates for the protein-protein case. The protocol fits into two 3-h sessions, leverages eight-channel parallel acquisition for efficient titrations, and includes safety notes, optional regeneration to demonstrate sensor reuse, and troubleshooting for non-ideal behaviors (drift, avidity, non-specific binding). Assessment artifacts (rubrics, short-answer prompts, and figure-quality criteria) support measurement of learning outcomes in experimental design and quantitative analysis. Because the same BSA surface underpins both assays, the module is cost effective, reproducible, and readily transferable across institutions, providing a practical framework for teaching how analyte size and mechanism shape biosensor strategy and interpretation. |
| Abstractor: | As Provided |
| Entry Date: | 2026 |
| Accession Number: | EJ1506860 |
| Database: | ERIC |
| FullText | Text: Availability: 0 |
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| Header | DbId: eric DbLabel: ERIC An: EJ1506860 AccessLevel: 3 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Teaching Bio-Layer Interferometry: Protein-Protein versus Protein-Small Molecule Interaction Analysis on an Octet R8 – Name: Language Label: Language Group: Lang Data: English – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wen+Chen%22">Wen Chen</searchLink> (ORCID <externalLink term="https://orcid.org/0000-0001-7387-4532">0000-0001-7387-4532</externalLink>) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="SO" term="%22Biochemistry+and+Molecular+Biology+Education%22"><i>Biochemistry and Molecular Biology Education</i></searchLink>. 2026 54(3):297-307. – Name: Avail Label: Availability Group: Avail Data: Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us – Name: PeerReviewed Label: Peer Reviewed Group: SrcInfo Data: Y – Name: Pages Label: Page Count Group: Src Data: 11 – Name: DatePubCY Label: Publication Date Group: Date Data: 2026 – Name: TypeDocument Label: Document Type Group: TypDoc Data: Journal Articles<br />Reports - Descriptive – Name: Audience Label: Education Level Group: Audnce Data: <searchLink fieldCode="EL" term="%22Higher+Education%22">Higher Education</searchLink><br /><searchLink fieldCode="EL" term="%22Postsecondary+Education%22">Postsecondary Education</searchLink> – Name: Subject Label: Descriptors Group: Su Data: <searchLink fieldCode="DE" term="%22College+Science%22">College Science</searchLink><br /><searchLink fieldCode="DE" term="%22Undergraduate+Study%22">Undergraduate Study</searchLink><br /><searchLink fieldCode="DE" term="%22Science+Activities%22">Science Activities</searchLink><br /><searchLink fieldCode="DE" term="%22Science+Instruction%22">Science Instruction</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+Biology%22">Molecular Biology</searchLink> – Name: DOI Label: DOI Group: ID Data: 10.1002/bmb.70049 – Name: ISSN Label: ISSN Group: ISSN Data: 1470-8175<br />1539-3429 – Name: Abstract Label: Abstract Group: Ab Data: We describe a classroom-ready bio-layer interferometry protocol for upper-level undergraduates that contrasts a macromolecular interaction with a small-molecule interaction on an Octet R8. Biotinylated bovine serum albumin (BSA) is immobilized on Super Streptavidin sensors to enable two assays in parallel: (i) binding of a commercially available anti-BSA IgG (protein-protein) and (ii) binding of a long-chain fatty acid such as palmitate (protein-small molecule). The exercise emphasizes assay design decisions that differ by analyte class-sensor chemistry and loading density, solvent matching and reference subtraction, association/dissociation timing, and the choice of data-analysis pathway (global 1:1 kinetic fitting for IgG vs. steady-state isotherms for fatty acids). Representative data yield nanomolar K[subscript D] values for BSA-IgG and micromolar apparent K[subscript D] for BSA-fatty acid, with correspondingly larger response amplitudes and slower off-rates for the protein-protein case. The protocol fits into two 3-h sessions, leverages eight-channel parallel acquisition for efficient titrations, and includes safety notes, optional regeneration to demonstrate sensor reuse, and troubleshooting for non-ideal behaviors (drift, avidity, non-specific binding). Assessment artifacts (rubrics, short-answer prompts, and figure-quality criteria) support measurement of learning outcomes in experimental design and quantitative analysis. Because the same BSA surface underpins both assays, the module is cost effective, reproducible, and readily transferable across institutions, providing a practical framework for teaching how analyte size and mechanism shape biosensor strategy and interpretation. – Name: AbstractInfo Label: Abstractor Group: Ab Data: As Provided – Name: DateEntry Label: Entry Date Group: Date Data: 2026 – Name: AN Label: Accession Number Group: ID Data: EJ1506860 |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=eric&AN=EJ1506860 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/bmb.70049 Languages: – Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 297 Subjects: – SubjectFull: College Science Type: general – SubjectFull: Undergraduate Study Type: general – SubjectFull: Science Activities Type: general – SubjectFull: Science Instruction Type: general – SubjectFull: Molecular Biology Type: general Titles: – TitleFull: Teaching Bio-Layer Interferometry: Protein-Protein versus Protein-Small Molecule Interaction Analysis on an Octet R8 Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wen Chen IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 1470-8175 – Type: issn-electronic Value: 1539-3429 Numbering: – Type: volume Value: 54 – Type: issue Value: 3 Titles: – TitleFull: Biochemistry and Molecular Biology Education Type: main |
| ResultId | 1 |
