Primer reporte de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en Aedes aegypti de Colombia.
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| Title: | Primer reporte de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en Aedes aegypti de Colombia. |
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| Alternate Title: | First report of the F1534C mutation, associated with cross-resistance to DDTand Pyrethroids, in Aedes aegypti from Colombia. |
| Authors: | Atencia, María Claudia1 mariclau_1112@hotmail.com, Pérez, María de Jesús1, Jaramillo, María Cristina1, Caldera, Sandy Milena1, Cochero, Suljey2, Bejarano, Eduar Elías1 |
| Source: | Biomédica: Revista del Instituto Nacional de Salud. sep2016, Vol. 36 Issue 3, p1-20. 20p. |
| Abstract (English): | Introduction: The principal strategy used to control the mosquito Aedes aegypti, which transmits the dengue, chikungunya and Zika viruses, is based on the use of insecticides with the aim of reducing vector populations. However, their use has led to the insect developing resistance to these chemicals. Objective: To determinate the presence of the F1534C mutation associated with cross-resistance to DDT and pyrethroids, in Ae. aegypti from the Colombian city of Sincelejo. Materials and methods: The study was carried out with nine specimens of Ae. aegypti that showed resistance to lambdacyhalothrin in bioassays developed by the Secretaría de Salud de Sucre. A semi-nested PCR, as previously described by Harris et al. (2010) was performed to amplify exon 31 of the para gene of the voltage-dependent sodium channel of Ae. aegypti. PCR products were sequenced, edited, and analyzed with the MEGA 5 software. Results: The wild-type allele and mutant of exon 31 was detected in all of the nine mosquitoes tested. Substitution of thymine for guanine was observed in the nucleotide sequence of the mutant allele, producing a change in the UUC codon to UGC and leading to the replacement of the aminoacid phenylalanine by cysteine in residue 1534 of the protein. Conclusion: The nine mosquitoes analyzed presented a heterozygote genotype for the F1534C mutation, whose phenotypic effect is knockdown resistance (kdr) to the insecticides DDT and pyrethroids. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Introducción. La principal estrategia para el control de Aedes aegypti, vector de los virus dengue, chikungunya y Zika, se basa en la utilización de insecticidas con el fin de disminuir las poblaciones del vector. Sin embargo, su uso ha conllevado a que el insecto desarrolle resistencia a estos químicos. Objetivo. Determinar la presencia de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en mosquitos de la especie Ae. aegypti de la ciudad de Sincelejo, Colombia. Materiales y métodos. El estudio se desarrolló con nueve ejemplares de Ae. aegypti que mostraron resistencia a lambdacialotrina en bioensayos desarrollados por la Secretaría de Salud de Sucre. Se realizó una PCR semi-anidada, según la la metodología descrita por Harris y colaboradores (2010), con el fin de amplificar el exón 31 del gen para del canal de sodio dependiente de voltaje de Ae. aegypti. Los productos de PCR se secuenciaron, editaron y analizaron con el programa MEGA 5. Resultados. En todos los mosquitos evaluados se detectó la presencia del alelo silvestre y mutante del exón 31. En la secuencia nucleotídica del alelo mutante, se observó una sustitución de timina por guanina, que produce el cambio del codón UUC por UGC y conlleva al reemplazo del aminoácido fenilalanina por cisteína en el residuo 1534 de la proteína. Conclusión. Los nueve mosquitos analizados presentaron un genotipo heterocigoto para la mutación F1534C, cuyo efecto fenotípico es la resistencia al derribo (kdr) por los insecticidas DDT y piretroides. [ABSTRACT FROM AUTHOR] |
| Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
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| Header | DbId: lth DbLabel: MedicLatina An: 115468291 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Primer reporte de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en Aedes aegypti de Colombia. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: First report of the F1534C mutation, associated with cross-resistance to DDTand Pyrethroids, in Aedes aegypti from Colombia. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Atencia%2C+María+Claudia%22">Atencia, María Claudia</searchLink><relatesTo>1</relatesTo><i> mariclau_1112@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Pérez%2C+María+de+Jesús%22">Pérez, María de Jesús</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Jaramillo%2C+María+Cristina%22">Jaramillo, María Cristina</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Caldera%2C+Sandy+Milena%22">Caldera, Sandy Milena</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cochero%2C+Suljey%22">Cochero, Suljey</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Bejarano%2C+Eduar+Elías%22">Bejarano, Eduar Elías</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biomédica%3A+Revista+del+Instituto+Nacional+de+Salud%22">Biomédica: Revista del Instituto Nacional de Salud</searchLink>. sep2016, Vol. 36 Issue 3, p1-20. 20p. – Name: Abstract Label: Abstract (English) Group: Ab Data: Introduction: The principal strategy used to control the mosquito Aedes aegypti, which transmits the dengue, chikungunya and Zika viruses, is based on the use of insecticides with the aim of reducing vector populations. However, their use has led to the insect developing resistance to these chemicals. Objective: To determinate the presence of the F1534C mutation associated with cross-resistance to DDT and pyrethroids, in Ae. aegypti from the Colombian city of Sincelejo. Materials and methods: The study was carried out with nine specimens of Ae. aegypti that showed resistance to lambdacyhalothrin in bioassays developed by the Secretaría de Salud de Sucre. A semi-nested PCR, as previously described by Harris et al. (2010) was performed to amplify exon 31 of the para gene of the voltage-dependent sodium channel of Ae. aegypti. PCR products were sequenced, edited, and analyzed with the MEGA 5 software. Results: The wild-type allele and mutant of exon 31 was detected in all of the nine mosquitoes tested. Substitution of thymine for guanine was observed in the nucleotide sequence of the mutant allele, producing a change in the UUC codon to UGC and leading to the replacement of the aminoacid phenylalanine by cysteine in residue 1534 of the protein. Conclusion: The nine mosquitoes analyzed presented a heterozygote genotype for the F1534C mutation, whose phenotypic effect is knockdown resistance (kdr) to the insecticides DDT and pyrethroids. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Introducción. La principal estrategia para el control de Aedes aegypti, vector de los virus dengue, chikungunya y Zika, se basa en la utilización de insecticidas con el fin de disminuir las poblaciones del vector. Sin embargo, su uso ha conllevado a que el insecto desarrolle resistencia a estos químicos. Objetivo. Determinar la presencia de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en mosquitos de la especie Ae. aegypti de la ciudad de Sincelejo, Colombia. Materiales y métodos. El estudio se desarrolló con nueve ejemplares de Ae. aegypti que mostraron resistencia a lambdacialotrina en bioensayos desarrollados por la Secretaría de Salud de Sucre. Se realizó una PCR semi-anidada, según la la metodología descrita por Harris y colaboradores (2010), con el fin de amplificar el exón 31 del gen para del canal de sodio dependiente de voltaje de Ae. aegypti. Los productos de PCR se secuenciaron, editaron y analizaron con el programa MEGA 5. Resultados. En todos los mosquitos evaluados se detectó la presencia del alelo silvestre y mutante del exón 31. En la secuencia nucleotídica del alelo mutante, se observó una sustitución de timina por guanina, que produce el cambio del codón UUC por UGC y conlleva al reemplazo del aminoácido fenilalanina por cisteína en el residuo 1534 de la proteína. Conclusión. Los nueve mosquitos analizados presentaron un genotipo heterocigoto para la mutación F1534C, cuyo efecto fenotípico es la resistencia al derribo (kdr) por los insecticidas DDT y piretroides. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 20 StartPage: 1 Titles: – TitleFull: Primer reporte de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en Aedes aegypti de Colombia. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Atencia, María Claudia – PersonEntity: Name: NameFull: Pérez, María de Jesús – PersonEntity: Name: NameFull: Jaramillo, María Cristina – PersonEntity: Name: NameFull: Caldera, Sandy Milena – PersonEntity: Name: NameFull: Cochero, Suljey – PersonEntity: Name: NameFull: Bejarano, Eduar Elías IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: sep2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 01204157 Numbering: – Type: volume Value: 36 – Type: issue Value: 3 Titles: – TitleFull: Biomédica: Revista del Instituto Nacional de Salud Type: main |
| ResultId | 1 |
