Utilidad de la microscopia holográfica digital para el estudio histomorfométrico de eritrocitos humanos.
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| Title: | Utilidad de la microscopia holográfica digital para el estudio histomorfométrico de eritrocitos humanos. |
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| Alternate Title: | Usefulness of the digital holographic microscopy for the histomorphometric study of human red blood cells. |
| Authors: | Infante Tavio, Nadia Inés1 ninfante@medinew.scu.sld.cu, Escalona Veloz, Rafael2, Sierra Calzado, C. Lillian1, Palacios Roque, Guillermo3 |
| Source: | MEDISAN. 2016, Vol. 20 Issue 11, p5096-5104. 9p. |
| Abstract (English): | A descriptive and cross-sectional investigation to determine the usefulness of the digital holographic microscopy in the histomorphometric study of human red blood cells, by means of the comparative analysis with the optic microscopy, was carried out in the Digital Holography Department of Oriente University in Santiago de Cuba, from April, 2014 to the same month in 2015. To such an effect, red blood cells samples diluted in the Hepes and Hepa shock-absorbing solutions were selected, Holodig program and Matlab® system were used for the measurement in the holographic technique, and in the statistical processing the Student t test was applied for the comparison of means between both microscopic techniques. It was concluded that the digital holographic microscopy allowed to observe the limits and contours of the studied red blood cells and the minimum variations in their form, besides carrying out morphometric studies which results showed similarity to those obtained with the optic microscopy, and of other parameters, as volume. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Se realizó una investigación descriptiva y transversal para determinar la utilidad de la microscopia holográfica digital en el estudio histomorfométrico de eritrocitos humanos, mediante el análisis comparativo con la microscopia óptica, en el Departamento de Holografía Digital de la Universidad de Oriente de Santiago de Cuba, de abril del 2014 a igual mes del 2015. A tal efecto se seleccionaron muestras de eritrocitos diluidos en las soluciones amortiguadoras Hepes y Hepa, se emplearon el programa Holodig® y el sistema Matlab® para las mediciones en la técnica holográfica, y en el procesamiento estadístico se aplicó la prueba de la t de Student para la comparación de medias entre ambas técnicas microscópicas. Se obtuvo que la microscopia holográfica digital permitiera observar los límites y contornos de los eritrocitos estudiados y las mínimas variaciones en su forma, además de realizar estudios morfométricos, cuyos resultados mostraron similitud a los obtenidos con la microscopia óptica, y de otros parámetros, como el volumen. [ABSTRACT FROM AUTHOR] |
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| Database: | MedicLatina |
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