IDENTIFICACIÓN Y DETERMINACIÓN ESTRUCTURAL DE UN SESQUITERPENO DE LAS HOJAS DE Tessaria integrifolia Ruiz & Pav. Y EVALUACIÓN DE SU ACTIVIDAD LEISHMANICIDA.
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| Title: | IDENTIFICACIÓN Y DETERMINACIÓN ESTRUCTURAL DE UN SESQUITERPENO DE LAS HOJAS DE Tessaria integrifolia Ruiz & Pav. Y EVALUACIÓN DE SU ACTIVIDAD LEISHMANICIDA. |
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| Alternate Title: | IDENTIFICATION AND STRUCTURAL DETERMINATION OF A SESQUITERPENE OF Tessaria integrifolia Ruiz & Pav. LEAVES AND EVALUATION OF ITS LEISHMANICIDAL ACTIVITY. |
| Authors: | Silva-Correa, Carmen R.1 csilva@unitru.edu.pe, Cruzado-Razco, José L.1, González-Blas, María V.1, García-Armas, Juan M.2, Ruiz-Reyes, Segundo G.1, Villarreal-La Torre, Víctor E.1, Gamarra-Sánchez, César D.1 |
| Source: | Revista Peruana de Medicina Experimental y Salud Pública. 2018, Vol. 35 Issue 2, p221-227. 7p. 1 Color Photograph, 2 Diagrams, 1 Chart, 1 Graph. |
| Abstract (English): | Objective. To identify and determine the phytoconstituent structure of Tessaria integrifolia Ruiz & Pav. leaves with leishmanicidal activity. Materials and Methods. Fluid extract of leaves was prepared, concentrated to soft extract, and used to evaluate leishmanicidal activity in Mesocricetus auratus with experimental leishmaniasis, at the dose of 250 mg/ kg of soft extract by intramuscular route for 15 days. Extract was fractionated in 45 cm column chromatography with a 2.5 cm diameter, containing G-60 silica gel, and 70-230 mesh (Sigma-Aldrich®). Nine fractions were obtained and assessed on macrophages infected with Leishmania sp to determine the active fraction and isolate the active compound, by separation, purification, and crystallization, analyzed by nuclear magnetic resonance (NMR) of 1H, 13C, and liquid chromatography coupled to mass spectroscopy (LC/ MS). Results. Fluid extract from the leaves of T. integrifolia presents leishmanicidal activity in M. auratus. Fraction F8 is active on infected macrophages at a dose of 14 μg/mL. An eudesman type sesquiterpene ((4aS, 5R, 6R, 8aR) -6-hydroxy-5, 8a-dimethyl-3- (1-methylethylidene) octahydronaphthalen-2 (1H) - one) was identified, by RMN 1 H, 13C, and LC / MS analysis. Conclusions. Fluid extract of leaves of Tessaria integrifolia Ruiz & Pav. presents leishmanicidal activity on Mesocricetus auratus with experimental leishmaniasis. Fraction F8 presents leishmanicidal activity on infected macrophages at a dose of 14 μg/mL. An eudesman type sesquiterpene was identified, according to 1 H, 13C, and LC / MS NMR analysis. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Objetivos. Identificar y determinar la estructura del fitoconstituyente de hojas de Tessaria integrifolia Ruiz & Pav con efecto leishmanicida. Materiales y métodos. Se preparó un extracto fluido de hojas de Tessaria integrifolia Ruiz & Pav. que fue concentrado a extracto blando y se utilizó para evaluar la actividad leishmanicida en Mesocricetus auratus con leishmaniasis experimental, administrando vía intramuscular la dosis de 250 mg/kg del extracto blando por 15 días. El extracto fue fraccionado en columna cromatográfica de 45 cm, con un diámetro de 2,5 cm que contiene silicagel G-60, de 70-230 mesh (Sigma-Aldrich®), las nueve fracciones obtenidas fueron evaluadas sobre macrófagos infectados con Leishmania sp para determinar la fracción activa y aislar el compuesto activo, mediante separación, purificación y cristalización, siendo analizado por resonancia magnética nuclear (RMN) de 1H, 13C, y cromatografía líquida acoplada a espectroscopía de masas (LC/MS). Resultados. El extracto fluido de las hojas de Tessaria integrifolia Ruiz & Pav. presenta actividad leishmanicida en Mesocricetus auratus y la fracción F8 es activa sobre macrófagos infectados a dosis de 14 μg/mL. Se elucidó en esa fracción un sesquiterpeno tipo eudesmano ((4aS, 5R,6R,8aR)-6-hidroxi-5,8a-dimetil-3- (1-metiletiliden) octahidronaftalen-2(1H)-ona), según análisis de RMN 1H, 13C, y LC/MS. Conclusiones. El extracto fluido de hojas de Tessaria integrifolia Ruiz & Pav. presenta actividad leishmanicida sobre Mesocricetus auratus. La fracción F8 presenta actividad leishmanicida sobre macrófagos infectados a dosis de 14 μg/mL. Se elucidó en la fracción activa un sesquiterpeno tipo eudesmano según los análisis de RMN 1H, 13C, y LC/MS. [ABSTRACT FROM AUTHOR] |
| Copyright of Revista Peruana de Medicina Experimental y Salud Pública is the property of Instituto Nacional de Salud (Peru) and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Items | – Name: Title Label: Title Group: Ti Data: IDENTIFICACIÓN Y DETERMINACIÓN ESTRUCTURAL DE UN SESQUITERPENO DE LAS HOJAS DE Tessaria integrifolia Ruiz & Pav. Y EVALUACIÓN DE SU ACTIVIDAD LEISHMANICIDA. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: IDENTIFICATION AND STRUCTURAL DETERMINATION OF A SESQUITERPENE OF Tessaria integrifolia Ruiz & Pav. LEAVES AND EVALUATION OF ITS LEISHMANICIDAL ACTIVITY. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Silva-Correa%2C+Carmen+R%2E%22">Silva-Correa, Carmen R.</searchLink><relatesTo>1</relatesTo><i> csilva@unitru.edu.pe</i><br /><searchLink fieldCode="AR" term="%22Cruzado-Razco%2C+José+L%2E%22">Cruzado-Razco, José L.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22González-Blas%2C+María+V%2E%22">González-Blas, María V.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22García-Armas%2C+Juan+M%2E%22">García-Armas, Juan M.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Ruiz-Reyes%2C+Segundo+G%2E%22">Ruiz-Reyes, Segundo G.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Villarreal-La+Torre%2C+Víctor+E%2E%22">Villarreal-La Torre, Víctor E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Gamarra-Sánchez%2C+César+D%2E%22">Gamarra-Sánchez, César D.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Revista+Peruana+de+Medicina+Experimental+y+Salud+Pública%22">Revista Peruana de Medicina Experimental y Salud Pública</searchLink>. 2018, Vol. 35 Issue 2, p221-227. 7p. 1 Color Photograph, 2 Diagrams, 1 Chart, 1 Graph. – Name: Abstract Label: Abstract (English) Group: Ab Data: Objective. To identify and determine the phytoconstituent structure of Tessaria integrifolia Ruiz & Pav. leaves with leishmanicidal activity. Materials and Methods. Fluid extract of leaves was prepared, concentrated to soft extract, and used to evaluate leishmanicidal activity in Mesocricetus auratus with experimental leishmaniasis, at the dose of 250 mg/ kg of soft extract by intramuscular route for 15 days. Extract was fractionated in 45 cm column chromatography with a 2.5 cm diameter, containing G-60 silica gel, and 70-230 mesh (Sigma-Aldrich®). Nine fractions were obtained and assessed on macrophages infected with Leishmania sp to determine the active fraction and isolate the active compound, by separation, purification, and crystallization, analyzed by nuclear magnetic resonance (NMR) of 1H, 13C, and liquid chromatography coupled to mass spectroscopy (LC/ MS). Results. Fluid extract from the leaves of T. integrifolia presents leishmanicidal activity in M. auratus. Fraction F8 is active on infected macrophages at a dose of 14 μg/mL. An eudesman type sesquiterpene ((4aS, 5R, 6R, 8aR) -6-hydroxy-5, 8a-dimethyl-3- (1-methylethylidene) octahydronaphthalen-2 (1H) - one) was identified, by RMN 1 H, 13C, and LC / MS analysis. Conclusions. Fluid extract of leaves of Tessaria integrifolia Ruiz & Pav. presents leishmanicidal activity on Mesocricetus auratus with experimental leishmaniasis. Fraction F8 presents leishmanicidal activity on infected macrophages at a dose of 14 μg/mL. An eudesman type sesquiterpene was identified, according to 1 H, 13C, and LC / MS NMR analysis. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Objetivos. Identificar y determinar la estructura del fitoconstituyente de hojas de Tessaria integrifolia Ruiz & Pav con efecto leishmanicida. Materiales y métodos. Se preparó un extracto fluido de hojas de Tessaria integrifolia Ruiz & Pav. que fue concentrado a extracto blando y se utilizó para evaluar la actividad leishmanicida en Mesocricetus auratus con leishmaniasis experimental, administrando vía intramuscular la dosis de 250 mg/kg del extracto blando por 15 días. El extracto fue fraccionado en columna cromatográfica de 45 cm, con un diámetro de 2,5 cm que contiene silicagel G-60, de 70-230 mesh (Sigma-Aldrich®), las nueve fracciones obtenidas fueron evaluadas sobre macrófagos infectados con Leishmania sp para determinar la fracción activa y aislar el compuesto activo, mediante separación, purificación y cristalización, siendo analizado por resonancia magnética nuclear (RMN) de 1H, 13C, y cromatografía líquida acoplada a espectroscopía de masas (LC/MS). Resultados. El extracto fluido de las hojas de Tessaria integrifolia Ruiz & Pav. presenta actividad leishmanicida en Mesocricetus auratus y la fracción F8 es activa sobre macrófagos infectados a dosis de 14 μg/mL. Se elucidó en esa fracción un sesquiterpeno tipo eudesmano ((4aS, 5R,6R,8aR)-6-hidroxi-5,8a-dimetil-3- (1-metiletiliden) octahidronaftalen-2(1H)-ona), según análisis de RMN 1H, 13C, y LC/MS. Conclusiones. El extracto fluido de hojas de Tessaria integrifolia Ruiz & Pav. presenta actividad leishmanicida sobre Mesocricetus auratus. La fracción F8 presenta actividad leishmanicida sobre macrófagos infectados a dosis de 14 μg/mL. Se elucidó en la fracción activa un sesquiterpeno tipo eudesmano según los análisis de RMN 1H, 13C, y LC/MS. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Revista Peruana de Medicina Experimental y Salud Pública is the property of Instituto Nacional de Salud (Peru) and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.17843/rpmesp.2018.352.3140 Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 7 StartPage: 221 Titles: – TitleFull: IDENTIFICACIÓN Y DETERMINACIÓN ESTRUCTURAL DE UN SESQUITERPENO DE LAS HOJAS DE Tessaria integrifolia Ruiz & Pav. Y EVALUACIÓN DE SU ACTIVIDAD LEISHMANICIDA. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Silva-Correa, Carmen R. – PersonEntity: Name: NameFull: Cruzado-Razco, José L. – PersonEntity: Name: NameFull: González-Blas, María V. – PersonEntity: Name: NameFull: García-Armas, Juan M. – PersonEntity: Name: NameFull: Ruiz-Reyes, Segundo G. – PersonEntity: Name: NameFull: Villarreal-La Torre, Víctor E. – PersonEntity: Name: NameFull: Gamarra-Sánchez, César D. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: 2018 Type: published Y: 2018 Identifiers: – Type: issn-print Value: 17264634 Numbering: – Type: volume Value: 35 – Type: issue Value: 2 Titles: – TitleFull: Revista Peruana de Medicina Experimental y Salud Pública Type: main |
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