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Detección molecular de leptospiras patógenas en roedores sinantrópicos y silvestres capturados en Yucatán, México.

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Title: Detección molecular de leptospiras patógenas en roedores sinantrópicos y silvestres capturados en Yucatán, México.
Alternate Title: Molecular detection of pathogenic Leptospira in synanthropic and wild rodents captured in Yucatán, México.
Authors: Torres-Castro, Marco1 torres@correo.uady.mx, Cruz-Camargo, Bayron1, Medina-Pinto, Rodrigo2, Reyes-Hernández, Bibiana1, Moguel-Lehmer, Carlos3, Medina, Rolando4, Ortiz-Esquivel, José2, Arcila-Fuentes, William2, López-Ávila, Armando1, Noh-Pech, Henry1, Panti-May, Alonso5, Rodríguez-Vivas, Iván2, Puerto, Fernando I.1
Source: Biomédica: Revista del Instituto Nacional de Salud. 2018 Supplement, Vol. 38, p51-58. 8p.
Abstract (English): Introduction: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira, which is endemic in México and considered a public and veterinary health problem. Rodents are the most relevant reservoirs of Leptospira spp. because the bacteria establish and reproduce in its renal tissue and are excreted through the urine. Objective: To identify the presence of Leptospira spp. in renal tissue from rodents captured in Yucatán, México. Materials and methods: Synanthropic and wild rodents were captured in the rural municipality of Cenotillo, Yucatán, México. We collected one kidney from each rodent and extracted the total DNA. The identification of Leptospira spp. was done by detecting two fragments of the 16S rRNA gene using end-point polymerase chain reaction (PCR). We sequenced and analyzed positive products using alignment tools. Results: A total of 92 rodents belonging to seven different species were captured. The PCR yielded a global positivity of 5.4% (5/92). The alignment analysis of the sequenced products demonstrated a 100% of coverage and identity with Leptospira interrogans. This is the first molecular evidence of Leptospira spp. circulation in Heteromys gaumeri captured in Yucatán, México. Conclusion: Our results evidenced that rodents of Yucatán are reservoirs of Leptospira spp. and participate in the infection cycle of leptospirosis in the region. [ABSTRACT FROM AUTHOR]
Abstract (Spanish): Introducción. La leptospirosis es una enfermedad zoonótica endémica en México, ocasionada por la bacteria del género Leptospira, la cual constituye un problema de salud pública y veterinaria. Los roedores son los reservorios más relevantes de Leptospira spp., debido a que la bacteria se establece y se reproduce en su tejido renal y es excretada por la orina. Objetivo. Identificar la presencia de Leptospira spp. en tejido renal de roedores capturados en Yucatán, México. Materiales y métodos. Se capturaron roedores sinantrópicos y silvestres en el municipio rural de Cenotillo, Yucatán, México. Se tomó un riñón de cada roedor y se extrajo el ADN total. La identificación de Leptospira spp. se hizo mediante la detección de dos fragmentos del gen 16S rRNA con una reacción en cadena de la polimerasa (PCR) de punto final. Los productos positivos se secuenciaron y se analizaron con herramientas de alineamiento. Resultados. Se capturaron 92 roedores pertenecientes a siete especies distintas. La PCR arrojó 5,4 % (5/92) de positividad global. El análisis del alineamiento de los aislamientos de los roedores infectados demostró 100 % de cobertura e identidad con la especie Leptospira interrogans. Esta es la primera evidencia molecular de la circulación de Leptospira spp. en Heteromys gaumeri capturados en Yucatán, México. Conclusión. Se evidenció que los roedores de Yucatán, México, son reservorios de Leptospira spp. y participan en el ciclo de infección de la leptospirosis en la región. [ABSTRACT FROM AUTHOR]
Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: MedicLatina
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  Data: Detección molecular de leptospiras patógenas en roedores sinantrópicos y silvestres capturados en Yucatán, México.
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  Data: Molecular detection of pathogenic Leptospira in synanthropic and wild rodents captured in Yucatán, México.
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  Data: <searchLink fieldCode="AR" term="%22Torres-Castro%2C+Marco%22">Torres-Castro, Marco</searchLink><relatesTo>1</relatesTo><i> torres@correo.uady.mx</i><br /><searchLink fieldCode="AR" term="%22Cruz-Camargo%2C+Bayron%22">Cruz-Camargo, Bayron</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Medina-Pinto%2C+Rodrigo%22">Medina-Pinto, Rodrigo</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Reyes-Hernández%2C+Bibiana%22">Reyes-Hernández, Bibiana</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Moguel-Lehmer%2C+Carlos%22">Moguel-Lehmer, Carlos</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Medina%2C+Rolando%22">Medina, Rolando</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Ortiz-Esquivel%2C+José%22">Ortiz-Esquivel, José</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Arcila-Fuentes%2C+William%22">Arcila-Fuentes, William</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22López-Ávila%2C+Armando%22">López-Ávila, Armando</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Noh-Pech%2C+Henry%22">Noh-Pech, Henry</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Panti-May%2C+Alonso%22">Panti-May, Alonso</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Rodríguez-Vivas%2C+Iván%22">Rodríguez-Vivas, Iván</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Puerto%2C+Fernando+I%2E%22">Puerto, Fernando I.</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Biomédica%3A+Revista+del+Instituto+Nacional+de+Salud%22">Biomédica: Revista del Instituto Nacional de Salud</searchLink>. 2018 Supplement, Vol. 38, p51-58. 8p.
– Name: Abstract
  Label: Abstract (English)
  Group: Ab
  Data: Introduction: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira, which is endemic in México and considered a public and veterinary health problem. Rodents are the most relevant reservoirs of Leptospira spp. because the bacteria establish and reproduce in its renal tissue and are excreted through the urine. Objective: To identify the presence of Leptospira spp. in renal tissue from rodents captured in Yucatán, México. Materials and methods: Synanthropic and wild rodents were captured in the rural municipality of Cenotillo, Yucatán, México. We collected one kidney from each rodent and extracted the total DNA. The identification of Leptospira spp. was done by detecting two fragments of the 16S rRNA gene using end-point polymerase chain reaction (PCR). We sequenced and analyzed positive products using alignment tools. Results: A total of 92 rodents belonging to seven different species were captured. The PCR yielded a global positivity of 5.4% (5/92). The alignment analysis of the sequenced products demonstrated a 100% of coverage and identity with Leptospira interrogans. This is the first molecular evidence of Leptospira spp. circulation in Heteromys gaumeri captured in Yucatán, México. Conclusion: Our results evidenced that rodents of Yucatán are reservoirs of Leptospira spp. and participate in the infection cycle of leptospirosis in the region. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label: Abstract (Spanish)
  Group: Ab
  Data: Introducción. La leptospirosis es una enfermedad zoonótica endémica en México, ocasionada por la bacteria del género Leptospira, la cual constituye un problema de salud pública y veterinaria. Los roedores son los reservorios más relevantes de Leptospira spp., debido a que la bacteria se establece y se reproduce en su tejido renal y es excretada por la orina. Objetivo. Identificar la presencia de Leptospira spp. en tejido renal de roedores capturados en Yucatán, México. Materiales y métodos. Se capturaron roedores sinantrópicos y silvestres en el municipio rural de Cenotillo, Yucatán, México. Se tomó un riñón de cada roedor y se extrajo el ADN total. La identificación de Leptospira spp. se hizo mediante la detección de dos fragmentos del gen 16S rRNA con una reacción en cadena de la polimerasa (PCR) de punto final. Los productos positivos se secuenciaron y se analizaron con herramientas de alineamiento. Resultados. Se capturaron 92 roedores pertenecientes a siete especies distintas. La PCR arrojó 5,4 % (5/92) de positividad global. El análisis del alineamiento de los aislamientos de los roedores infectados demostró 100 % de cobertura e identidad con la especie Leptospira interrogans. Esta es la primera evidencia molecular de la circulación de Leptospira spp. en Heteromys gaumeri capturados en Yucatán, México. Conclusión. Se evidenció que los roedores de Yucatán, México, son reservorios de Leptospira spp. y participan en el ciclo de infección de la leptospirosis en la región. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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