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Evaluación del daño oxidativo y por metilación del ADN de pintores expuestos ocupacionalmente a solventes orgánicos y pinturas.

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Title: Evaluación del daño oxidativo y por metilación del ADN de pintores expuestos ocupacionalmente a solventes orgánicos y pinturas.
Alternate Title: Evaluation of oxidative and methylating DNA damage in painters occupationally exposed to organic solvents and paints.
Authors: Londoño-Velasco, Elizabeth1,2 elivelasco@javerianacali.edu.co, Martínez-Perafán, Fabián1,3, Carvaja, Silvio1, García-Vallejo, Felipe4, Hoyos-Giraldo, Luz Stella1
Source: Biomédica: Revista del Instituto Nacional de Salud. sep2019, Vol. 39 Issue 3, p464-477. 14p.
Subjects: DNA damage, INDUSTRIAL safety, ORGANIC solvents, INFORMAL sector, GENETIC toxicology
Abstract (English): Introduction: The exposure to organic solvents and paints has been associated with genotoxicity and a greater risk of neoplasms. However, the type of DNA damage induced in humans by the exposure to these compounds, which would help explain the mechanisms of their genotoxicity, is still not fully characterized. Due to inadequate practices of occupational safety, car painters in the informal sector are a highly exposed group to organic solvents and paints. Objective: To identify the oxidative and methylating damage in the DNA of lymphocytes of car painters exposed to organic solvents and paints. Materials and methods: Isolated peripheral blood lymphocytes from 62 painters and 62 unexposed subjects were analyzed by the modified high-throughput comet assay with the Fpg and AlkA enzymes. The categories used for the evaluation of the DNA damage were basal damage (without enzymes), oxidative and methylating damage. The measurement parameter used to establish the damage was the percentage of DNA in the tail. Results: The percentage of DNA in the tail was higher in the exposed group compared to the unexposed group (p<0.05). In the exposed group, this percentage was higher in the oxidative damage category than the baseline (16.50 vs. 12.87; p<0.001), whereas methylating damage did not show significant differences (14.00 vs. 12.87; p>0.05).Conclusion: In this study, exposure to organic solvents and paints was associated with an increase in oxidative lesions in the DNA of car painters' lymphocytes, such as the production of 8-oxodG and other formamidopyrimidine products which are considered highly mutagenic. [ABSTRACT FROM AUTHOR]
Abstract (Spanish): Introducción. La exposición a solventes orgánicos y pinturas se ha asociado con efectos genotóxicos y mayor riesgo de neoplasias. Sin embargo, aún no se ha caracterizado bien el tipo de daño que esta exposición induce en el ADN humano, ni los mecanismos por los cuales se genera. Uno de los grupos con mayor exposición a dichos solventes y pinturas son los pintores de automóviles del sector informal que trabajan sin adecuadas prácticas de seguridad ocupacional. Objetivo. Determinar el daño oxidativo y por metilación del ADN de linfocitos de pintores de automóviles expuestos a solventes orgánicos y pinturas. Materiales y métodos. Se analizaron linfocitos aislados de sangre periférica de 62 pintores y 62 sujetos no expuestos mediante el ensayo cometa de gran eficiencia acoplado a las enzimas Fpg y AlkA. Las categorías de daño en el ADN evaluadas fueron el daño basal (sin enzimas), el daño oxidativo y el daño por metilación, y el parámetro de medición, el porcentaje de ADN en la cola. Resultados. El porcentaje de ADN en la cola fue mayor en el grupo expuesto con respecto al no expuesto (p<0,05). En el grupo expuesto, dicho porcentaje fue mayor en la categoría de daño oxidativo comparado con la del basal (16,50 Vs. 12,87; p<0,001), en tanto que en el daño por metilación no se encontraron diferencias significativas (14,00 Vs. 12,87; p>0,05). Conclusión. La exposición a solventes orgánicos y pinturas se asoció con el aumento de las lesiones oxidativas del ADN de los linfocitos de pintores de automóviles, tales como la producción de 8-oxo-2'-desoxiguanosina (8-oxodG) y otros productos formamidopirimidina, los cuales se consideran considerablemente mutagénicos. [ABSTRACT FROM AUTHOR]
Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: MedicLatina
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  Data: Evaluation of oxidative and methylating DNA damage in painters occupationally exposed to organic solvents and paints.
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  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22DNA+damage%22&quot;&gt;DNA damage&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22INDUSTRIAL+safety%22&quot;&gt;INDUSTRIAL safety&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22ORGANIC+solvents%22&quot;&gt;ORGANIC solvents&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22INFORMAL+sector%22&quot;&gt;INFORMAL sector&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22GENETIC+toxicology%22&quot;&gt;GENETIC toxicology&lt;/searchLink&gt;
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  Data: Introduction: The exposure to organic solvents and paints has been associated with genotoxicity and a greater risk of neoplasms. However, the type of DNA damage induced in humans by the exposure to these compounds, which would help explain the mechanisms of their genotoxicity, is still not fully characterized. Due to inadequate practices of occupational safety, car painters in the informal sector are a highly exposed group to organic solvents and paints. Objective: To identify the oxidative and methylating damage in the DNA of lymphocytes of car painters exposed to organic solvents and paints. Materials and methods: Isolated peripheral blood lymphocytes from 62 painters and 62 unexposed subjects were analyzed by the modified high-throughput comet assay with the Fpg and AlkA enzymes. The categories used for the evaluation of the DNA damage were basal damage (without enzymes), oxidative and methylating damage. The measurement parameter used to establish the damage was the percentage of DNA in the tail. Results: The percentage of DNA in the tail was higher in the exposed group compared to the unexposed group (p&lt;0.05). In the exposed group, this percentage was higher in the oxidative damage category than the baseline (16.50 vs. 12.87; p&lt;0.001), whereas methylating damage did not show significant differences (14.00 vs. 12.87; p&gt;0.05).Conclusion: In this study, exposure to organic solvents and paints was associated with an increase in oxidative lesions in the DNA of car painters&#39; lymphocytes, such as the production of 8-oxodG and other formamidopyrimidine products which are considered highly mutagenic. [ABSTRACT FROM AUTHOR]
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  Data: Introducci&#243;n. La exposici&#243;n a solventes org&#225;nicos y pinturas se ha asociado con efectos genot&#243;xicos y mayor riesgo de neoplasias. Sin embargo, a&#250;n no se ha caracterizado bien el tipo de da&#241;o que esta exposici&#243;n induce en el ADN humano, ni los mecanismos por los cuales se genera. Uno de los grupos con mayor exposici&#243;n a dichos solventes y pinturas son los pintores de autom&#243;viles del sector informal que trabajan sin adecuadas pr&#225;cticas de seguridad ocupacional. Objetivo. Determinar el da&#241;o oxidativo y por metilaci&#243;n del ADN de linfocitos de pintores de autom&#243;viles expuestos a solventes org&#225;nicos y pinturas. Materiales y m&#233;todos. Se analizaron linfocitos aislados de sangre perif&#233;rica de 62 pintores y 62 sujetos no expuestos mediante el ensayo cometa de gran eficiencia acoplado a las enzimas Fpg y AlkA. Las categor&#237;as de da&#241;o en el ADN evaluadas fueron el da&#241;o basal (sin enzimas), el da&#241;o oxidativo y el da&#241;o por metilaci&#243;n, y el par&#225;metro de medici&#243;n, el porcentaje de ADN en la cola. Resultados. El porcentaje de ADN en la cola fue mayor en el grupo expuesto con respecto al no expuesto (p&lt;0,05). En el grupo expuesto, dicho porcentaje fue mayor en la categor&#237;a de da&#241;o oxidativo comparado con la del basal (16,50 Vs. 12,87; p&lt;0,001), en tanto que en el da&#241;o por metilaci&#243;n no se encontraron diferencias significativas (14,00 Vs. 12,87; p&gt;0,05). Conclusi&#243;n. La exposici&#243;n a solventes org&#225;nicos y pinturas se asoci&#243; con el aumento de las lesiones oxidativas del ADN de los linfocitos de pintores de autom&#243;viles, tales como la producci&#243;n de 8-oxo-2&#39;-desoxiguanosina (8-oxodG) y otros productos formamidopirimidina, los cuales se consideran considerablemente mutag&#233;nicos. [ABSTRACT FROM AUTHOR]
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  Data: &lt;i&gt;Copyright of Biom&#233;dica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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        Value: 10.7705/biomedica.4289
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        Text: Spanish
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