Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae).
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| Title: | Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae). |
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| Alternate Title: | Establecimiento del hongo entomopatógeno Beauveria bassiana como endófito en Capsicum annuum y sus efectos sobre la plaga del áfido Myzus persicae (Homoptera: Aphididae). |
| Authors: | Allegrucci, Natalia1 nataliaallegrucci@yahoo.com, Silvana Velazquez, Maria1 mariasilvanavelazquez@gmail.com, Leticia Russo, María1 leticia_russo@hotmail.com, Florencia Vianna, Maria1 florvianna@yahoo.com.ar, Abarca, Camila1 camila.abrc@gmail.com, Clara Scorsetti, Ana1 anacscorsetti@yahoo.com |
| Source: | Revista de Biología Tropical. dec2020, Vol. 68 Issue 4, p1084-1094. 11p. |
| Subjects: | ENTOMOPATHOGENIC fungi, BEAUVERIA bassiana, GREEN peach aphid, CAPSICUM annuum, INSECT nematodes, BIOLOGICAL pest control agents, HOMOPTERA, APHIDS |
| Abstract (English): | Introduction: Entomopathogenic fungi have been used as biological control agents of insect pests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species. Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassiana inoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassiana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference and growth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping. The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B. bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant statistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonization frequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a low presence. This might indicate the occurrence of antifungal activity that interferes with the establishment of the entomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepper leaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We did not find significant differences in the feeding preference between inoculated and control leaves, although there was a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strain B. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effective inoculation technique was leaf spraying. We did not find differences of statistical significance with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluating the indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to further explore their potential use in the management of insect pests. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Introducción: Los hongos entomopatógenos se han utilizado como agentes de control biológico de plagas de insectos y han demostrado una capacidad prometedora para colonizar una amplia gama de especies de plantas como endófitos. Objetivo: Los objetivos de este estudio fueron: evaluar las técnicas de inoculación más efectivas para B. bassiana en plantas de pimiento; evaluar la presencia de endófitos naturales y su interacción con B. bassiana y evaluar el efecto de B. bassiana sobre la preferencia de alimentación de la hoja de pimiento y el crecimiento de la plaga de áfidos M. persicae. Métodos: Se evaluaron tres técnicas de inoculación: aspersión foliar, inmersión de semillas e inmersión de raíces. Las plantas de pimiento se inocularon con una suspensión de conidias ajustada a 1x108 conidias/ml. La presencia de B. bassiana en las plántulas se evaluó a los 7, 15 y 28 días después de la inoculación. Resultados: Se encontraron diferencias significativas entre las técnicas de inoculación. La aspersión foliar mostró la frecuencia más alta. Los endófitos naturales presentaron frecuencias más altas en los tratamientos en los que B. bassiana tuvo una frecuencia baja, esto podría indicar la presencia de actividad antifúngica que interfiere con el establecimiento del entomopatógeno en los tejidos de pimiento. No se encontraron diferencias significativas en la mortalidad, reproducción y preferencia alimentaria de M. persicae. Conclusiones: Se demostró que la cepa B. bassiana LPSC 1067 se estableció efectivamente como endófito en los tejidos de pimiento. La técnica de inoculación más efectiva fue la aspersión foliar. No se encontraron diferencias significativas con respecto a la mortalidad, reproducción y preferencia alimentaria de M. persicae. Sin embargo, la inoculación con B. bassiana mostró una tendencia a aumentar la mortalidad de los adultos y a disminuir la descendencia (ninfas). Se necesitan estudios futuros que evalúen los efectos indirectos de los entomopatógenos endofíticos tanto en plantas como en insectos herbívoros para explorar más a fondo su posible uso en el manejo de insectos plaga. [ABSTRACT FROM AUTHOR] |
| Copyright of Revista de Biología Tropical is the property of Universidad de Costa Rica and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Items | – Name: Title Label: Title Group: Ti Data: Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae). – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: Establecimiento del hongo entomopatógeno Beauveria bassiana como endófito en Capsicum annuum y sus efectos sobre la plaga del áfido Myzus persicae (Homoptera: Aphididae). – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Allegrucci%2C+Natalia%22">Allegrucci, Natalia</searchLink><relatesTo>1</relatesTo><i> nataliaallegrucci@yahoo.com</i><br /><searchLink fieldCode="AR" term="%22Silvana+Velazquez%2C+Maria%22">Silvana Velazquez, Maria</searchLink><relatesTo>1</relatesTo><i> mariasilvanavelazquez@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Leticia+Russo%2C+María%22">Leticia Russo, María</searchLink><relatesTo>1</relatesTo><i> leticia_russo@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Florencia+Vianna%2C+Maria%22">Florencia Vianna, Maria</searchLink><relatesTo>1</relatesTo><i> florvianna@yahoo.com.ar</i><br /><searchLink fieldCode="AR" term="%22Abarca%2C+Camila%22">Abarca, Camila</searchLink><relatesTo>1</relatesTo><i> camila.abrc@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Clara+Scorsetti%2C+Ana%22">Clara Scorsetti, Ana</searchLink><relatesTo>1</relatesTo><i> anacscorsetti@yahoo.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Revista+de+Biología+Tropical%22">Revista de Biología Tropical</searchLink>. dec2020, Vol. 68 Issue 4, p1084-1094. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22ENTOMOPATHOGENIC+fungi%22">ENTOMOPATHOGENIC fungi</searchLink><br /><searchLink fieldCode="DE" term="%22BEAUVERIA+bassiana%22">BEAUVERIA bassiana</searchLink><br /><searchLink fieldCode="DE" term="%22GREEN+peach+aphid%22">GREEN peach aphid</searchLink><br /><searchLink fieldCode="DE" term="%22CAPSICUM+annuum%22">CAPSICUM annuum</searchLink><br /><searchLink fieldCode="DE" term="%22INSECT+nematodes%22">INSECT nematodes</searchLink><br /><searchLink fieldCode="DE" term="%22BIOLOGICAL+pest+control+agents%22">BIOLOGICAL pest control agents</searchLink><br /><searchLink fieldCode="DE" term="%22HOMOPTERA%22">HOMOPTERA</searchLink><br /><searchLink fieldCode="DE" term="%22APHIDS%22">APHIDS</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: Introduction: Entomopathogenic fungi have been used as biological control agents of insect pests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species. Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassiana inoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassiana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference and growth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping. The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B. bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant statistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonization frequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a low presence. This might indicate the occurrence of antifungal activity that interferes with the establishment of the entomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepper leaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We did not find significant differences in the feeding preference between inoculated and control leaves, although there was a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strain B. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effective inoculation technique was leaf spraying. We did not find differences of statistical significance with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluating the indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to further explore their potential use in the management of insect pests. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Introducción: Los hongos entomopatógenos se han utilizado como agentes de control biológico de plagas de insectos y han demostrado una capacidad prometedora para colonizar una amplia gama de especies de plantas como endófitos. Objetivo: Los objetivos de este estudio fueron: evaluar las técnicas de inoculación más efectivas para B. bassiana en plantas de pimiento; evaluar la presencia de endófitos naturales y su interacción con B. bassiana y evaluar el efecto de B. bassiana sobre la preferencia de alimentación de la hoja de pimiento y el crecimiento de la plaga de áfidos M. persicae. Métodos: Se evaluaron tres técnicas de inoculación: aspersión foliar, inmersión de semillas e inmersión de raíces. Las plantas de pimiento se inocularon con una suspensión de conidias ajustada a 1x108 conidias/ml. La presencia de B. bassiana en las plántulas se evaluó a los 7, 15 y 28 días después de la inoculación. Resultados: Se encontraron diferencias significativas entre las técnicas de inoculación. La aspersión foliar mostró la frecuencia más alta. Los endófitos naturales presentaron frecuencias más altas en los tratamientos en los que B. bassiana tuvo una frecuencia baja, esto podría indicar la presencia de actividad antifúngica que interfiere con el establecimiento del entomopatógeno en los tejidos de pimiento. No se encontraron diferencias significativas en la mortalidad, reproducción y preferencia alimentaria de M. persicae. Conclusiones: Se demostró que la cepa B. bassiana LPSC 1067 se estableció efectivamente como endófito en los tejidos de pimiento. La técnica de inoculación más efectiva fue la aspersión foliar. No se encontraron diferencias significativas con respecto a la mortalidad, reproducción y preferencia alimentaria de M. persicae. Sin embargo, la inoculación con B. bassiana mostró una tendencia a aumentar la mortalidad de los adultos y a disminuir la descendencia (ninfas). Se necesitan estudios futuros que evalúen los efectos indirectos de los entomopatógenos endofíticos tanto en plantas como en insectos herbívoros para explorar más a fondo su posible uso en el manejo de insectos plaga. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Revista de Biología Tropical is the property of Universidad de Costa Rica and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.15517/rbt.v68i4.41218 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 1084 Subjects: – SubjectFull: ENTOMOPATHOGENIC fungi Type: general – SubjectFull: BEAUVERIA bassiana Type: general – SubjectFull: GREEN peach aphid Type: general – SubjectFull: CAPSICUM annuum Type: general – SubjectFull: INSECT nematodes Type: general – SubjectFull: BIOLOGICAL pest control agents Type: general – SubjectFull: HOMOPTERA Type: general – SubjectFull: APHIDS Type: general Titles: – TitleFull: Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae). Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Allegrucci, Natalia – PersonEntity: Name: NameFull: Silvana Velazquez, Maria – PersonEntity: Name: NameFull: Leticia Russo, María – PersonEntity: Name: NameFull: Florencia Vianna, Maria – PersonEntity: Name: NameFull: Abarca, Camila – PersonEntity: Name: NameFull: Clara Scorsetti, Ana IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: dec2020 Type: published Y: 2020 Identifiers: – Type: issn-print Value: 00347744 Numbering: – Type: volume Value: 68 – Type: issue Value: 4 Titles: – TitleFull: Revista de Biología Tropical Type: main |
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