View in EDS PDF Full Text

NEUTRALIZATION OF THE LETHAL ACTIVITY FROM Bothrops atrox VENOM BY HYPERIMUNE LLAMA SERUM (Lama glama)

Saved in:
Bibliographic Details
Title: NEUTRALIZATION OF THE LETHAL ACTIVITY FROM Bothrops atrox VENOM BY HYPERIMUNE LLAMA SERUM (Lama glama)
Authors: Calderon, Henri Bailon1 (AUTHOR) hbailon@ins.gob.pe, Colque Alave, Elizabeth Gaby1 (AUTHOR), Yaniro Coronel, Verónica Olga1 (AUTHOR), Padilla Rojas, Carlos1 (AUTHOR), Galarza Pérez, Marco1 (AUTHOR), Cáceres Rey, Omar Alberto1 (AUTHOR), Bonilla Ferreyra, César2 (AUTHOR), Felix, Benigno Tintaya2 (AUTHOR), García Neyra, David1 (AUTHOR), Inga Arellano, Rosalina Rosio2 (AUTHOR), Ormachea, Silvia Seraylan2 (AUTHOR), Arevalo, Harrison Montejo1 (AUTHOR)
Source: Revista Peruana de Medicina Experimental y Salud Pública. 2020, Vol. 37 Issue 3, p446-453. 8p. 1 Chart, 3 Graphs.
Abstract: Objectives: To evaluate the capacity of the hyperimmune llama serum (Lama glama) to neutralize the lethal activity of Bothrops atrox venom in laboratory mice. Materials and methods: Mean lethal dose (LD50) was calculated from a Bothrops atrox venom sample pool from Peru. The antibody titers were measured by ELISA assay; and the immune serum neutralization potency was measured by calculating the mean effective dose (ED50) during the immunization period. Results: The venom’s LD50 was 3.96 μg/g; similar to what was found in other studies about Bothrops atrox carried out in Peru. The titers of antibodies against the venom increased rapidly in the llama, demonstrating a fast immune response; however, the neutralization capacity increased slowly and required several doses and immunization reinforcements, obtaining a ED50 of 3.30 μL/g mouse and a neutralization potency of 3.6 mg/mL after 15 immunizations. Conclusions: The hyperimmune llama serum is able to neutralize the lethality of the Bothrops atrox venom from Peru in laboratory mice. [ABSTRACT FROM AUTHOR]
Copyright of Revista Peruana de Medicina Experimental y Salud Pública is the property of Instituto Nacional de Salud (Peru) and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: MedicLatina
FullText Links:
  – Type: pdflink
Text:
  Availability: 0
Header DbId: lth
DbLabel: MedicLatina
An: 147994947
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: NEUTRALIZATION OF THE LETHAL ACTIVITY FROM Bothrops atrox VENOM BY HYPERIMUNE LLAMA SERUM (Lama glama)
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Calderon%2C+Henri+Bailon%22">Calderon, Henri Bailon</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> hbailon@ins.gob.pe</i><br /><searchLink fieldCode="AR" term="%22Colque+Alave%2C+Elizabeth+Gaby%22">Colque Alave, Elizabeth Gaby</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yaniro+Coronel%2C+Verónica+Olga%22">Yaniro Coronel, Verónica Olga</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Padilla+Rojas%2C+Carlos%22">Padilla Rojas, Carlos</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Galarza+Pérez%2C+Marco%22">Galarza Pérez, Marco</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cáceres+Rey%2C+Omar+Alberto%22">Cáceres Rey, Omar Alberto</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bonilla+Ferreyra%2C+César%22">Bonilla Ferreyra, César</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Felix%2C+Benigno+Tintaya%22">Felix, Benigno Tintaya</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22García+Neyra%2C+David%22">García Neyra, David</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Inga+Arellano%2C+Rosalina+Rosio%22">Inga Arellano, Rosalina Rosio</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ormachea%2C+Silvia+Seraylan%22">Ormachea, Silvia Seraylan</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Arevalo%2C+Harrison+Montejo%22">Arevalo, Harrison Montejo</searchLink><relatesTo>1</relatesTo> (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Revista+Peruana+de+Medicina+Experimental+y+Salud+Pública%22">Revista Peruana de Medicina Experimental y Salud Pública</searchLink>. 2020, Vol. 37 Issue 3, p446-453. 8p. 1 Chart, 3 Graphs.
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Objectives: To evaluate the capacity of the hyperimmune llama serum (Lama glama) to neutralize the lethal activity of Bothrops atrox venom in laboratory mice. Materials and methods: Mean lethal dose (LD50) was calculated from a Bothrops atrox venom sample pool from Peru. The antibody titers were measured by ELISA assay; and the immune serum neutralization potency was measured by calculating the mean effective dose (ED50) during the immunization period. Results: The venom’s LD50 was 3.96 μg/g; similar to what was found in other studies about Bothrops atrox carried out in Peru. The titers of antibodies against the venom increased rapidly in the llama, demonstrating a fast immune response; however, the neutralization capacity increased slowly and required several doses and immunization reinforcements, obtaining a ED50 of 3.30 μL/g mouse and a neutralization potency of 3.6 mg/mL after 15 immunizations. Conclusions: The hyperimmune llama serum is able to neutralize the lethality of the Bothrops atrox venom from Peru in laboratory mice. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Revista Peruana de Medicina Experimental y Salud Pública is the property of Instituto Nacional de Salud (Peru) and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lth&AN=147994947
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.17843/rpmesp.2020.373.4597
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 8
        StartPage: 446
    Titles:
      – TitleFull: NEUTRALIZATION OF THE LETHAL ACTIVITY FROM Bothrops atrox VENOM BY HYPERIMUNE LLAMA SERUM (Lama glama)
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Calderon, Henri Bailon
      – PersonEntity:
          Name:
            NameFull: Colque Alave, Elizabeth Gaby
      – PersonEntity:
          Name:
            NameFull: Yaniro Coronel, Verónica Olga
      – PersonEntity:
          Name:
            NameFull: Padilla Rojas, Carlos
      – PersonEntity:
          Name:
            NameFull: Galarza Pérez, Marco
      – PersonEntity:
          Name:
            NameFull: Cáceres Rey, Omar Alberto
      – PersonEntity:
          Name:
            NameFull: Bonilla Ferreyra, César
      – PersonEntity:
          Name:
            NameFull: Felix, Benigno Tintaya
      – PersonEntity:
          Name:
            NameFull: García Neyra, David
      – PersonEntity:
          Name:
            NameFull: Inga Arellano, Rosalina Rosio
      – PersonEntity:
          Name:
            NameFull: Ormachea, Silvia Seraylan
      – PersonEntity:
          Name:
            NameFull: Arevalo, Harrison Montejo
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 07
              Text: 2020
              Type: published
              Y: 2020
          Identifiers:
            – Type: issn-print
              Value: 17264634
          Numbering:
            – Type: volume
              Value: 37
            – Type: issue
              Value: 3
          Titles:
            – TitleFull: Revista Peruana de Medicina Experimental y Salud Pública
              Type: main
ResultId 1