View in EDS PDF Full Text

Distribución y caracterización fenotípica y genotípica de Listeria monocytogenes en aislamientos de alimentos, Colombia, 2010-2018.

Saved in:
Bibliographic Details
Title: Distribución y caracterización fenotípica y genotípica de Listeria monocytogenes en aislamientos de alimentos, Colombia, 2010-2018.
Alternate Title: Distribution and phenotypic and genotypic characterization of Listeria monocytogenes isolated from food, Colombia, 2010-2018.
Authors: Isabel Muñoz, Ana1 anisabel2001@yahoo.com, Catering Rodríguez, Edna1
Source: Biomédica: Revista del Instituto Nacional de Salud. 2021 Supplement, Vol. 41, p165-179. 17p.
Subjects: PULSED-field gel electrophoresis, SERODIAGNOSIS, LISTERIA monocytogenes, PREPARED foods, GENOTYPES
Geographic Terms: BOGOTA (Colombia)
Abstract (English): Introduction: Listeria monocytogenes is a food-borne pathogen that may cause infections in humans such as meningitis, meningoencephalitis, and septicemia, as well as abortions. By serological typing 13 serotypes have been identified of which 4b is responsible for most of the outbreaks in the world. Objective: To determine the frequency and distribution of serotypes and molecular subtypes of L. monocytogenes isolated in Colombia from food from 2010 to 2018. Materials and methods: We conducted a retrospective and descriptive study based on the analysis of 2,420 isolates confirmed as L. monocytogenes and other species using biochemical and serological tests, and pulsed-field gel electrophoresis (PFGE) for molecular subtyping. Results: Of the 2,420 isolates received, 2,326 were confirmed as L. monocytogenes. The serotypes found were 4b (52%), 4d-4e (14.5%), 1/2a (11%), 1/2c (9.4%), 1/2b (9%), and 3a, 3b, 3c, 4c, 4d, 4e and 7 (less than 2%). The isolates came from Bogotá (43%), Antioquia (25%), Valle (10%), Nariño (9%), and other departments (7%). The genotypic characterization grouped the isolates in 167 PFGE patterns. The most frequent patterns were identified in various dairy and meat products, and in prepared foods. Conclusion: A 96.1% of the isolates corresponded to L. monocytogenes showing good agreement between isolates and identification. Serotype 4b, highly virulent, was the most frequent. The molecular analysis showed the possible dissemination and permanence over time of several serotypes, which highlights the importance of including this pathogen in epidemiological food surveillance programs. [ABSTRACT FROM AUTHOR]
Abstract (Spanish): Introducción. Listeria monocytogenes es un patógeno transmitido por alimentos que causa infecciones en humanos, entre ellas, meningitis, meningoencefalitis y septicemias, así como abortos. Con la tipificación serológica se han identificado 13 serotipos, siendo el 4b el causante de la mayoría de los brotes en el mundo. Objetivo. Determinar la frecuencia y la distribución de los serotipos y subtipos moleculares de L. monocytogenes aislados de alimentos en Colombia entre el 2010 y el 2018. Materiales y métodos. Se hizo un estudio descriptivo y retrospectivo a partir del análisis de 2.420 aislamientos que fueron identificados como L. monocytogenes y otras especies, por medio de pruebas bioquímicas, serológicas y de subtipificación molecular mediante electroforesis en gel de campo pulsado (PFGE). Resultados. De los 2.420 aislamientos recibidos, 2.326 fueron confirmados como L. monocytogenes. Los serotipos encontrados fueron: 4b (52 %), 4d-4e (14,5 %), 1/2a (11 %), 1/2c (9,4 %), 1/2b (9 %), y 3a, 3b, 3c, 4c, 4d, 4e y 7 (menos de 2 %). Procedían de Bogotá (43 %), Antioquia (25 %), Valle (10 %), Nariño (9 %) y otros departamentos (7 %). La caracterización genotípica agrupó los aislamientos evaluados en 167 patrones de PFGE; los perfiles más frecuentes se presentaron en productos lácteos, cárnicos y alimentos preparados. Conclusión. El 96,1 % de los aislamientos correspondieron a L. monocytogenes, con una buena concordancia entre el aislamiento y la identificación; el serotipo 4b, extremadamente virulento, fue el más frecuente. El análisis molecular evidenció la posible diseminación y permanencia en el tiempo de varios serotipos, lo que resalta la importancia de incluir este patógeno en los programas de vigilancia epidemiológica en alimentos. [ABSTRACT FROM AUTHOR]
Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: MedicLatina
FullText Links:
  – Type: pdflink
Text:
  Availability: 0
Header DbId: lth
DbLabel: MedicLatina
An: 153772829
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Distribución y caracterización fenotípica y genotípica de Listeria monocytogenes en aislamientos de alimentos, Colombia, 2010-2018.
– Name: TitleAlt
  Label: Alternate Title
  Group: TiAlt
  Data: Distribution and phenotypic and genotypic characterization of Listeria monocytogenes isolated from food, Colombia, 2010-2018.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Isabel+Muñoz%2C+Ana%22">Isabel Muñoz, Ana</searchLink><relatesTo>1</relatesTo><i> anisabel2001@yahoo.com</i><br /><searchLink fieldCode="AR" term="%22Catering+Rodríguez%2C+Edna%22">Catering Rodríguez, Edna</searchLink><relatesTo>1</relatesTo>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Biomédica%3A+Revista+del+Instituto+Nacional+de+Salud%22">Biomédica: Revista del Instituto Nacional de Salud</searchLink>. 2021 Supplement, Vol. 41, p165-179. 17p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22PULSED-field+gel+electrophoresis%22">PULSED-field gel electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22SERODIAGNOSIS%22">SERODIAGNOSIS</searchLink><br /><searchLink fieldCode="DE" term="%22LISTERIA+monocytogenes%22">LISTERIA monocytogenes</searchLink><br /><searchLink fieldCode="DE" term="%22PREPARED+foods%22">PREPARED foods</searchLink><br /><searchLink fieldCode="DE" term="%22GENOTYPES%22">GENOTYPES</searchLink>
– Name: SubjectGeographic
  Label: Geographic Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22BOGOTA+%28Colombia%29%22">BOGOTA (Colombia)</searchLink>
– Name: Abstract
  Label: Abstract (English)
  Group: Ab
  Data: Introduction: Listeria monocytogenes is a food-borne pathogen that may cause infections in humans such as meningitis, meningoencephalitis, and septicemia, as well as abortions. By serological typing 13 serotypes have been identified of which 4b is responsible for most of the outbreaks in the world. Objective: To determine the frequency and distribution of serotypes and molecular subtypes of L. monocytogenes isolated in Colombia from food from 2010 to 2018. Materials and methods: We conducted a retrospective and descriptive study based on the analysis of 2,420 isolates confirmed as L. monocytogenes and other species using biochemical and serological tests, and pulsed-field gel electrophoresis (PFGE) for molecular subtyping. Results: Of the 2,420 isolates received, 2,326 were confirmed as L. monocytogenes. The serotypes found were 4b (52%), 4d-4e (14.5%), 1/2a (11%), 1/2c (9.4%), 1/2b (9%), and 3a, 3b, 3c, 4c, 4d, 4e and 7 (less than 2%). The isolates came from Bogotá (43%), Antioquia (25%), Valle (10%), Nariño (9%), and other departments (7%). The genotypic characterization grouped the isolates in 167 PFGE patterns. The most frequent patterns were identified in various dairy and meat products, and in prepared foods. Conclusion: A 96.1% of the isolates corresponded to L. monocytogenes showing good agreement between isolates and identification. Serotype 4b, highly virulent, was the most frequent. The molecular analysis showed the possible dissemination and permanence over time of several serotypes, which highlights the importance of including this pathogen in epidemiological food surveillance programs. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label: Abstract (Spanish)
  Group: Ab
  Data: Introducción. Listeria monocytogenes es un patógeno transmitido por alimentos que causa infecciones en humanos, entre ellas, meningitis, meningoencefalitis y septicemias, así como abortos. Con la tipificación serológica se han identificado 13 serotipos, siendo el 4b el causante de la mayoría de los brotes en el mundo. Objetivo. Determinar la frecuencia y la distribución de los serotipos y subtipos moleculares de L. monocytogenes aislados de alimentos en Colombia entre el 2010 y el 2018. Materiales y métodos. Se hizo un estudio descriptivo y retrospectivo a partir del análisis de 2.420 aislamientos que fueron identificados como L. monocytogenes y otras especies, por medio de pruebas bioquímicas, serológicas y de subtipificación molecular mediante electroforesis en gel de campo pulsado (PFGE). Resultados. De los 2.420 aislamientos recibidos, 2.326 fueron confirmados como L. monocytogenes. Los serotipos encontrados fueron: 4b (52 %), 4d-4e (14,5 %), 1/2a (11 %), 1/2c (9,4 %), 1/2b (9 %), y 3a, 3b, 3c, 4c, 4d, 4e y 7 (menos de 2 %). Procedían de Bogotá (43 %), Antioquia (25 %), Valle (10 %), Nariño (9 %) y otros departamentos (7 %). La caracterización genotípica agrupó los aislamientos evaluados en 167 patrones de PFGE; los perfiles más frecuentes se presentaron en productos lácteos, cárnicos y alimentos preparados. Conclusión. El 96,1 % de los aislamientos correspondieron a L. monocytogenes, con una buena concordancia entre el aislamiento y la identificación; el serotipo 4b, extremadamente virulento, fue el más frecuente. El análisis molecular evidenció la posible diseminación y permanencia en el tiempo de varios serotipos, lo que resalta la importancia de incluir este patógeno en los programas de vigilancia epidemiológica en alimentos. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lth&AN=153772829
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.7705/biomedica.6152
    Languages:
      – Code: spa
        Text: Spanish
    PhysicalDescription:
      Pagination:
        PageCount: 17
        StartPage: 165
    Subjects:
      – SubjectFull: PULSED-field gel electrophoresis
        Type: general
      – SubjectFull: SERODIAGNOSIS
        Type: general
      – SubjectFull: LISTERIA monocytogenes
        Type: general
      – SubjectFull: PREPARED foods
        Type: general
      – SubjectFull: GENOTYPES
        Type: general
      – SubjectFull: BOGOTA (Colombia)
        Type: general
    Titles:
      – TitleFull: Distribución y caracterización fenotípica y genotípica de Listeria monocytogenes en aislamientos de alimentos, Colombia, 2010-2018.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Isabel Muñoz, Ana
      – PersonEntity:
          Name:
            NameFull: Catering Rodríguez, Edna
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 02
              M: 10
              Text: 2021 Supplement
              Type: published
              Y: 2021
          Identifiers:
            – Type: issn-print
              Value: 01204157
          Numbering:
            – Type: volume
              Value: 41
          Titles:
            – TitleFull: Biomédica: Revista del Instituto Nacional de Salud
              Type: main
ResultId 1