La infección por Nocardia brasiliensis induce la muerte celular de macrófagos.

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Bibliographic Details
Title:	La infección por Nocardia brasiliensis induce la muerte celular de macrófagos.
Alternate Title:	Nocardia brasiliensis infection induces macrophage cell death.
Authors:	Navarro-Durán, Llary Laudith^1,2,3, Robles-Contreras, Atzin^4,5, Castrillón Rivera, Laura Estela², Palma-Ramos, Alejandro², Luna-Herrera, Julieta julietalunah@hotmail.com, Castañeda-Sánchez, Jorge Ismael² jcastanedas@correo.xoc.uam.mx
Source:	Dermatología Revista Mexicana. Mar/Apr2022, Vol. 66 Issue 2, p205-214. 10p.
Abstract (English):	OBJECTIVE: To determine cell death in macrophages infected with N. brasiliensis. MATERIALS AND METHODS: An experimental study done from January 2019 to December 2020, in which the J774A.1 macrophage cell line was used. Post-infection kinetics were performed at 48h with N. brasiliensis at a MOI of 10:1. At each postinfection time, the observation of morphological changes by optical microscopy was carried out, the determination of the cell death of the infected macrophages was carried out by NA/BrEt and Höechst/IP stains, additionally, quantification of macrophage death was determined by flow cytometry. RESULTS: An increase in macrophage death was found as the postinfection time increased. Quantification of cell death in uninfected macrophages showed no significant changes. NA/BrEt and Höechst/IP stains suggested different forms of cell death, the main were apoptosis and necrosis. CONCLUSIONS: The results suggest that macrophages infected by Nocardia brasiliensis die from apoptosis and necrosis. [ABSTRACT FROM AUTHOR]
Abstract (Spanish):	OBJETIVO: Determinar la muerte celular en macrófagos infectados con N. brasiliensis. MATERIALES Y MÉTODOS: Estudio experimental efectuado de enero de 2019 a diciembre de 2020, en el que se utilizó la línea celular de macrófagos J774A.1. Se realizaron cinéticas posinfección a 48 horas con N. brasiliensis a una MOI de 10:1. En cada tiempo posinfección se realizó la observación de cambios morfológicos por microscopia óptica, se determinó la muerte celular de los macrófagos infectados con tinciones de NA/BrEt y Höechst/IP, asimismo, se realizó la cuantificación de la muerte de los macrófagos utilizando citometría de flujo. RESULTADOS: Se encontró incremento en la muerte de los macrófagos a medida que aumentó el tiempo posinfección. La cuantificación de la muerte celular en macrófagos sin infectar no demostró cambios importantes. Las tinciones con NA/BrEt y Höechst// IP sugirieron que hubo diferentes formas de muerte celular macrofágica, entre las que destacaron apoptosis y necrosis. CONCLUSIONES: Los resultados sugieren que los macrófagos infectados por Nocardia brasiliensis mueren por apoptosis y necrosis. [ABSTRACT FROM AUTHOR]
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Database:	MedicLatina

Description
Abstract:	OBJECTIVE: To determine cell death in macrophages infected with N. brasiliensis. MATERIALS AND METHODS: An experimental study done from January 2019 to December 2020, in which the J774A.1 macrophage cell line was used. Post-infection kinetics were performed at 48h with N. brasiliensis at a MOI of 10:1. At each postinfection time, the observation of morphological changes by optical microscopy was carried out, the determination of the cell death of the infected macrophages was carried out by NA/BrEt and Höechst/IP stains, additionally, quantification of macrophage death was determined by flow cytometry. RESULTS: An increase in macrophage death was found as the postinfection time increased. Quantification of cell death in uninfected macrophages showed no significant changes. NA/BrEt and Höechst/IP stains suggested different forms of cell death, the main were apoptosis and necrosis. CONCLUSIONS: The results suggest that macrophages infected by Nocardia brasiliensis die from apoptosis and necrosis. [ABSTRACT FROM AUTHOR]
ISSN:	01854038
DOI:	10.24245/dermatolrevmex.v66i2.7625