La infección por Nocardia brasiliensis induce la muerte celular de macrófagos.
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| Title: | La infección por Nocardia brasiliensis induce la muerte celular de macrófagos. |
|---|---|
| Alternate Title: | Nocardia brasiliensis infection induces macrophage cell death. |
| Authors: | Navarro-Durán, Llary Laudith1,2,3, Robles-Contreras, Atzin4,5, Castrillón Rivera, Laura Estela2, Palma-Ramos, Alejandro2, Luna-Herrera, Julieta julietalunah@hotmail.com, Castañeda-Sánchez, Jorge Ismael2 jcastanedas@correo.xoc.uam.mx |
| Source: | Dermatología Revista Mexicana. Mar/Apr2022, Vol. 66 Issue 2, p205-214. 10p. |
| Abstract (English): | OBJECTIVE: To determine cell death in macrophages infected with N. brasiliensis. MATERIALS AND METHODS: An experimental study done from January 2019 to December 2020, in which the J774A.1 macrophage cell line was used. Post-infection kinetics were performed at 48h with N. brasiliensis at a MOI of 10:1. At each postinfection time, the observation of morphological changes by optical microscopy was carried out, the determination of the cell death of the infected macrophages was carried out by NA/BrEt and Höechst/IP stains, additionally, quantification of macrophage death was determined by flow cytometry. RESULTS: An increase in macrophage death was found as the postinfection time increased. Quantification of cell death in uninfected macrophages showed no significant changes. NA/BrEt and Höechst/IP stains suggested different forms of cell death, the main were apoptosis and necrosis. CONCLUSIONS: The results suggest that macrophages infected by Nocardia brasiliensis die from apoptosis and necrosis. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | OBJETIVO: Determinar la muerte celular en macrófagos infectados con N. brasiliensis. MATERIALES Y MÉTODOS: Estudio experimental efectuado de enero de 2019 a diciembre de 2020, en el que se utilizó la línea celular de macrófagos J774A.1. Se realizaron cinéticas posinfección a 48 horas con N. brasiliensis a una MOI de 10:1. En cada tiempo posinfección se realizó la observación de cambios morfológicos por microscopia óptica, se determinó la muerte celular de los macrófagos infectados con tinciones de NA/BrEt y Höechst/IP, asimismo, se realizó la cuantificación de la muerte de los macrófagos utilizando citometría de flujo. RESULTADOS: Se encontró incremento en la muerte de los macrófagos a medida que aumentó el tiempo posinfección. La cuantificación de la muerte celular en macrófagos sin infectar no demostró cambios importantes. Las tinciones con NA/BrEt y Höechst// IP sugirieron que hubo diferentes formas de muerte celular macrofágica, entre las que destacaron apoptosis y necrosis. CONCLUSIONES: Los resultados sugieren que los macrófagos infectados por Nocardia brasiliensis mueren por apoptosis y necrosis. [ABSTRACT FROM AUTHOR] |
| Copyright of Dermatología Revista Mexicana is the property of Edicion y Farmacia S.A. de C.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: lth DbLabel: MedicLatina An: 156951846 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: La infección por Nocardia brasiliensis induce la muerte celular de macrófagos. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: Nocardia brasiliensis infection induces macrophage cell death. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Navarro-Durán%2C+Llary+Laudith%22">Navarro-Durán, Llary Laudith</searchLink><relatesTo>1,2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Robles-Contreras%2C+Atzin%22">Robles-Contreras, Atzin</searchLink><relatesTo>4,5</relatesTo><br /><searchLink fieldCode="AR" term="%22Castrillón+Rivera%2C+Laura+Estela%22">Castrillón Rivera, Laura Estela</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Palma-Ramos%2C+Alejandro%22">Palma-Ramos, Alejandro</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Luna-Herrera%2C+Julieta%22">Luna-Herrera, Julieta</searchLink><i> julietalunah@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Castañeda-Sánchez%2C+Jorge+Ismael%22">Castañeda-Sánchez, Jorge Ismael</searchLink><relatesTo>2</relatesTo><i> jcastanedas@correo.xoc.uam.mx</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Dermatología+Revista+Mexicana%22">Dermatología Revista Mexicana</searchLink>. Mar/Apr2022, Vol. 66 Issue 2, p205-214. 10p. – Name: Abstract Label: Abstract (English) Group: Ab Data: OBJECTIVE: To determine cell death in macrophages infected with N. brasiliensis. MATERIALS AND METHODS: An experimental study done from January 2019 to December 2020, in which the J774A.1 macrophage cell line was used. Post-infection kinetics were performed at 48h with N. brasiliensis at a MOI of 10:1. At each postinfection time, the observation of morphological changes by optical microscopy was carried out, the determination of the cell death of the infected macrophages was carried out by NA/BrEt and Höechst/IP stains, additionally, quantification of macrophage death was determined by flow cytometry. RESULTS: An increase in macrophage death was found as the postinfection time increased. Quantification of cell death in uninfected macrophages showed no significant changes. NA/BrEt and Höechst/IP stains suggested different forms of cell death, the main were apoptosis and necrosis. CONCLUSIONS: The results suggest that macrophages infected by Nocardia brasiliensis die from apoptosis and necrosis. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: OBJETIVO: Determinar la muerte celular en macrófagos infectados con N. brasiliensis. MATERIALES Y MÉTODOS: Estudio experimental efectuado de enero de 2019 a diciembre de 2020, en el que se utilizó la línea celular de macrófagos J774A.1. Se realizaron cinéticas posinfección a 48 horas con N. brasiliensis a una MOI de 10:1. En cada tiempo posinfección se realizó la observación de cambios morfológicos por microscopia óptica, se determinó la muerte celular de los macrófagos infectados con tinciones de NA/BrEt y Höechst/IP, asimismo, se realizó la cuantificación de la muerte de los macrófagos utilizando citometría de flujo. RESULTADOS: Se encontró incremento en la muerte de los macrófagos a medida que aumentó el tiempo posinfección. La cuantificación de la muerte celular en macrófagos sin infectar no demostró cambios importantes. Las tinciones con NA/BrEt y Höechst// IP sugirieron que hubo diferentes formas de muerte celular macrofágica, entre las que destacaron apoptosis y necrosis. CONCLUSIONES: Los resultados sugieren que los macrófagos infectados por Nocardia brasiliensis mueren por apoptosis y necrosis. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Dermatología Revista Mexicana is the property of Edicion y Farmacia S.A. de C.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.24245/dermatolrevmex.v66i2.7625 Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 10 StartPage: 205 Titles: – TitleFull: La infección por Nocardia brasiliensis induce la muerte celular de macrófagos. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Navarro-Durán, Llary Laudith – PersonEntity: Name: NameFull: Robles-Contreras, Atzin – PersonEntity: Name: NameFull: Castrillón Rivera, Laura Estela – PersonEntity: Name: NameFull: Palma-Ramos, Alejandro – PersonEntity: Name: NameFull: Luna-Herrera, Julieta – PersonEntity: Name: NameFull: Castañeda-Sánchez, Jorge Ismael IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar/Apr2022 Type: published Y: 2022 Identifiers: – Type: issn-print Value: 01854038 Numbering: – Type: volume Value: 66 – Type: issue Value: 2 Titles: – TitleFull: Dermatología Revista Mexicana Type: main |
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