Frequency of Trichinella spiral is in blood and muscles of horses from two slaughter houses (industrial and rural) in the State of Mexico, Mexico.

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Bibliographic Details
Title:	Frequency of Trichinella spiral is in blood and muscles of horses from two slaughter houses (industrial and rural) in the State of Mexico, Mexico.
Alternate Title:	Frecuencia de Trichinella spiralis en sangre y músculo de equinos sacrificados en dos diferentes mataderos, uno de tipo industrial y otro de tipo rural en el Estado de México, México.
Authors:	Jiménez-Cardoso, Enedina¹ enedina@servidor.unam.mx, Caballero-García, María De Lourdes¹, Uribe-Gutiérrez, Gabriel¹, Trejo-Hernández, Esmeralda¹, Gay-Jiménez, Francisco Ramón² gayjfr@hotmail.com
Source:	Veterinaria México. jul-sep2005, Vol. 36 Issue 3, p269-278. 10p. 2 Diagrams, 2 Charts.
Subjects:	TRICHINELLA spiralis, HORSEMEAT, SLAUGHTERING, DIGESTION, POLYMERASE chain reaction
Abstract (English):	The objective of this study was the evaluation of the frequency of Trichinella spiralis in horse meat that is used for human and animal consumption. A total of 150 horses were sampled, from two private slaughterhouses, one industrial slaughterhouse with some level of technology and the other one rural with less technology. Seventy animals from the first one and 80 from the second one were sampled. After the horses were slaughtered 10 g were taken from three muscles of each horse: gluteus, masseter and diaphragm or tongue as well as 5 ml of blood. The muscle tissue was observed looking for Trichinella spiralis larvae by: a) trichinoscopy b) artificial digestion and c) polymerase chain reaction. This last technique was used also for the blood analysis. For both slaughterhouses, all muscles observed by trichinoscopy were negative. By artificial digestion analysis, T. spiralis larvae were found in the three muscles of only one horse of the 70 that came from the industrial slaughterhouse, which represents a 1.42% prevalence in this lot. Of the eighty horses of the rural slaughterhouse T. Spiralis was found in only the masseter of one horse and this represents 1.25% positiveness. With the polymerase chain reaction T. spiralis was identified in three muscles of one horse of the industrial slaughterhouse and in blood of another two horses which gives a total infection frequency of 4.28%. In the horses from the rural slaughterhouse, the polymerase chain reaction was positive in muscles of three horses; the total frequency of infection in this lot was 3.75%, taking into consideration that the results for blood with this technique came out negative. It is concluded that the frequency of T. spiralis was similar to what has been found in other reports, although there were differences between both slaughterhouses in relation to the parasitic burden of the animals. [ABSTRACT FROM AUTHOR]
Abstract (Spanish):	El objetivo de este estudio fue evaluar la frecuencia de Trichinella spiralis en carne de caballo que se utiliza para consumo humano y para animales. Se muestrearon 150 caballos procedentes de dos mataderos particulares, uno semitecnificado industrial y otro rural, 70 pertenecieron al primero y 80 al segundo; después de sacrificar a los animales, de cada caballo se tomaron 10 g de tres músculos; glúteo, masetero, diafragma o lengua, así como 5 ml de sangre; en la carne se buscó presencia de larvas de T. spiralis mediante: a) triquinoscopía, b) digestión artificial y c) reacción de la cadena de la polimerasa, esta última también fue utilizada en la sangre. Los resultados mostraron que la triquinoscopía fue negativa en todos los músculos evaluados de los dos mataderos. Por digestión artificial, en un caballo del matadero semitecnificado industrial se encontraron larvas de T. spiralis en los tres músculos con 1.42% de infección y en el músculo (masetero) de un caballo del matadero rural, por lo que la positividad de este ultimo fue de 1.25%. La reacción en cadena de la polimerasa fue positiva en los tres músculos de los caballos del matadero industrial y en la sangre de dos animales que resultaron positivos, dando una frecuencia total de infección de 4.8%. En el matadero rural la reacción en cadena de la polimerasa fue positiva en músculos de tres caballos, mientras que los análisis de sangre fueron negativos en todos los caballos evaluados indicando una frecuencia total de la infección de 3.75%. Se concluye que la frecuencia de T. spiralis fue alta y semejante entre los dos mataderos pero hubo diferencias en relación a su carga parasitaria. [ABSTRACT FROM AUTHOR]
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Database:	MedicLatina

Description
Abstract:	The objective of this study was the evaluation of the frequency of Trichinella spiralis in horse meat that is used for human and animal consumption. A total of 150 horses were sampled, from two private slaughterhouses, one industrial slaughterhouse with some level of technology and the other one rural with less technology. Seventy animals from the first one and 80 from the second one were sampled. After the horses were slaughtered 10 g were taken from three muscles of each horse: gluteus, masseter and diaphragm or tongue as well as 5 ml of blood. The muscle tissue was observed looking for Trichinella spiralis larvae by: a) trichinoscopy b) artificial digestion and c) polymerase chain reaction. This last technique was used also for the blood analysis. For both slaughterhouses, all muscles observed by trichinoscopy were negative. By artificial digestion analysis, T. spiralis larvae were found in the three muscles of only one horse of the 70 that came from the industrial slaughterhouse, which represents a 1.42% prevalence in this lot. Of the eighty horses of the rural slaughterhouse T. Spiralis was found in only the masseter of one horse and this represents 1.25% positiveness. With the polymerase chain reaction T. spiralis was identified in three muscles of one horse of the industrial slaughterhouse and in blood of another two horses which gives a total infection frequency of 4.28%. In the horses from the rural slaughterhouse, the polymerase chain reaction was positive in muscles of three horses; the total frequency of infection in this lot was 3.75%, taking into consideration that the results for blood with this technique came out negative. It is concluded that the frequency of T. spiralis was similar to what has been found in other reports, although there were differences between both slaughterhouses in relation to the parasitic burden of the animals. [ABSTRACT FROM AUTHOR]
ISSN:	03015092