DESARROLLO DE LA TROPOMIOSINA A RECOMBINANTE ANTIGÉNICA de Echinococcus granulosus EN UN SISTEMA BACTERIANO COMO CANDIDATO VACUNAL CONTRA LA EQUINOCOCOSIS CANINA.
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| Title: | DESARROLLO DE LA TROPOMIOSINA A RECOMBINANTE ANTIGÉNICA de Echinococcus granulosus EN UN SISTEMA BACTERIANO COMO CANDIDATO VACUNAL CONTRA LA EQUINOCOCOSIS CANINA. |
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| Alternate Title: | DEVELOPMENT OF THE ANTIGENIC RECOMBINANT TROPOMYOSIN OF Echinococcus granulosus IN A BACTERIAL SYSTEM AS A VACCINAL CANDIDATE AGAINST CANINE ECHINOCOCCOSIS. |
| Authors: | Acosta-Benites, Janet jamnethe5@gmail.com, Jara, Luis M., Verastegui Pimentel, Manuela, Obregón Maldonado, Pepe M., Altamirano-Zevallos, Faride, Valencia Mamani, Nicasio, Gavidia, Cesar M. |
| Source: | Revista Peruana de Medicina Experimental y Salud Pública. 2024, Vol. 41 Issue 4, p411-416. 6p. |
| Subjects: | RECOMBINANT proteins, ECHINOCOCCUS granulosus, ESCHERICHIA coli, TWO-dimensional electrophoresis, GENETIC vectors |
| Abstract (English): | This study aimed to clone, express and produce the recombinant Echinococcus granulosus tropomyosin isoform A protein (EgTrpA) that maintains its antigenic and immunogenic properties as a potential vaccine candidate for dogs and sheep. The Echinococcus granulosus tropomyosin protein (EgTrp) gene was cloned into two vectors: Tropo/His-tag [pET28a (+)] and Tropo/GST-tag (pGEX6P-1). It was then expressed in E. coli BL21. Protein identity was determined by two-dimensional electrophoresis. Immunogenicity and antigenicity were verified by immunizing rabbits with each recombinant protein and assessed by western blot and ELISA. Two-dimensional electrophoresis identified the recombinant EgTrp protein as isoform A. The recombinant proteins showed recognition reactions on Western Blot and serum from immunized rabbits showed an increase in Tropo/His-tag IgG antibodies similar to Tropo/GST-tag. The recombinant EgTrpA protein showed antigenic and immunogenic characteristics in laboratory animals. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | El objetivo de este estudio fue clonar, expresar y producir la proteína recombinante tropomiosina isoforma A de Echinococcus granulosus (EgTrpA) que mantenga sus propiedades antigénicas e inmunogénicas como posible candidata a vacuna para perros y ovejas. El gen de la proteína tropomiosina de Echinococcus granulosus (EgTrp), se clono en dos vectores: Tropo/His-tag [pET28a (+)] y Tropo/GST-tag (pGEX6P-1). Luego, se expresó en E. coli BL21. La identidad de la proteína se determinó mediante electroforesis bidimensional. La inmunogenicidad y la antigenicidad se verificó mediante la inmunización de conejos con cada proteína recombinante y se evaluó mediante Western Blot y ELISA. La electroforesis bidimensional identificó la proteína recombinante EgTrp como isoforma A. Las proteínas recombinantes mostraron reacciones de reconocimiento en el Western Blot y el suero de los conejos inmunizados mostró un aumento de los anticuerpos IgG de Tropo/His-tag similar a Tropo/GST-tag. La proteína recombinante EgTrpA demostró características antigénicas e inmunogénicas en animales de laboratorio. [ABSTRACT FROM AUTHOR] |
| Copyright of Revista Peruana de Medicina Experimental y Salud Pública is the property of Instituto Nacional de Salud (Peru) and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
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| Header | DbId: lth DbLabel: MedicLatina An: 181882683 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: DESARROLLO DE LA TROPOMIOSINA A RECOMBINANTE ANTIGÉNICA de Echinococcus granulosus EN UN SISTEMA BACTERIANO COMO CANDIDATO VACUNAL CONTRA LA EQUINOCOCOSIS CANINA. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: DEVELOPMENT OF THE ANTIGENIC RECOMBINANT TROPOMYOSIN OF Echinococcus granulosus IN A BACTERIAL SYSTEM AS A VACCINAL CANDIDATE AGAINST CANINE ECHINOCOCCOSIS. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Acosta-Benites%2C+Janet%22">Acosta-Benites, Janet</searchLink><i> jamnethe5@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Jara%2C+Luis+M%2E%22">Jara, Luis M.</searchLink><br /><searchLink fieldCode="AR" term="%22Verastegui+Pimentel%2C+Manuela%22">Verastegui Pimentel, Manuela</searchLink><br /><searchLink fieldCode="AR" term="%22Obregón+Maldonado%2C+Pepe+M%2E%22">Obregón Maldonado, Pepe M.</searchLink><br /><searchLink fieldCode="AR" term="%22Altamirano-Zevallos%2C+Faride%22">Altamirano-Zevallos, Faride</searchLink><br /><searchLink fieldCode="AR" term="%22Valencia+Mamani%2C+Nicasio%22">Valencia Mamani, Nicasio</searchLink><br /><searchLink fieldCode="AR" term="%22Gavidia%2C+Cesar+M%2E%22">Gavidia, Cesar M.</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Revista+Peruana+de+Medicina+Experimental+y+Salud+Pública%22">Revista Peruana de Medicina Experimental y Salud Pública</searchLink>. 2024, Vol. 41 Issue 4, p411-416. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22RECOMBINANT+proteins%22">RECOMBINANT proteins</searchLink><br /><searchLink fieldCode="DE" term="%22ECHINOCOCCUS+granulosus%22">ECHINOCOCCUS granulosus</searchLink><br /><searchLink fieldCode="DE" term="%22ESCHERICHIA+coli%22">ESCHERICHIA coli</searchLink><br /><searchLink fieldCode="DE" term="%22TWO-dimensional+electrophoresis%22">TWO-dimensional electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22GENETIC+vectors%22">GENETIC vectors</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: This study aimed to clone, express and produce the recombinant Echinococcus granulosus tropomyosin isoform A protein (EgTrpA) that maintains its antigenic and immunogenic properties as a potential vaccine candidate for dogs and sheep. The Echinococcus granulosus tropomyosin protein (EgTrp) gene was cloned into two vectors: Tropo/His-tag [pET28a (+)] and Tropo/GST-tag (pGEX6P-1). It was then expressed in E. coli BL21. Protein identity was determined by two-dimensional electrophoresis. Immunogenicity and antigenicity were verified by immunizing rabbits with each recombinant protein and assessed by western blot and ELISA. Two-dimensional electrophoresis identified the recombinant EgTrp protein as isoform A. The recombinant proteins showed recognition reactions on Western Blot and serum from immunized rabbits showed an increase in Tropo/His-tag IgG antibodies similar to Tropo/GST-tag. The recombinant EgTrpA protein showed antigenic and immunogenic characteristics in laboratory animals. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: El objetivo de este estudio fue clonar, expresar y producir la proteína recombinante tropomiosina isoforma A de Echinococcus granulosus (EgTrpA) que mantenga sus propiedades antigénicas e inmunogénicas como posible candidata a vacuna para perros y ovejas. El gen de la proteína tropomiosina de Echinococcus granulosus (EgTrp), se clono en dos vectores: Tropo/His-tag [pET28a (+)] y Tropo/GST-tag (pGEX6P-1). Luego, se expresó en E. coli BL21. La identidad de la proteína se determinó mediante electroforesis bidimensional. La inmunogenicidad y la antigenicidad se verificó mediante la inmunización de conejos con cada proteína recombinante y se evaluó mediante Western Blot y ELISA. La electroforesis bidimensional identificó la proteína recombinante EgTrp como isoforma A. Las proteínas recombinantes mostraron reacciones de reconocimiento en el Western Blot y el suero de los conejos inmunizados mostró un aumento de los anticuerpos IgG de Tropo/His-tag similar a Tropo/GST-tag. La proteína recombinante EgTrpA demostró características antigénicas e inmunogénicas en animales de laboratorio. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Revista Peruana de Medicina Experimental y Salud Pública is the property of Instituto Nacional de Salud (Peru) and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.17843/rpmesp.2024.414.13854 Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 6 StartPage: 411 Subjects: – SubjectFull: RECOMBINANT proteins Type: general – SubjectFull: ECHINOCOCCUS granulosus Type: general – SubjectFull: ESCHERICHIA coli Type: general – SubjectFull: TWO-dimensional electrophoresis Type: general – SubjectFull: GENETIC vectors Type: general Titles: – TitleFull: DESARROLLO DE LA TROPOMIOSINA A RECOMBINANTE ANTIGÉNICA de Echinococcus granulosus EN UN SISTEMA BACTERIANO COMO CANDIDATO VACUNAL CONTRA LA EQUINOCOCOSIS CANINA. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Acosta-Benites, Janet – PersonEntity: Name: NameFull: Jara, Luis M. – PersonEntity: Name: NameFull: Verastegui Pimentel, Manuela – PersonEntity: Name: NameFull: Obregón Maldonado, Pepe M. – PersonEntity: Name: NameFull: Altamirano-Zevallos, Faride – PersonEntity: Name: NameFull: Valencia Mamani, Nicasio – PersonEntity: Name: NameFull: Gavidia, Cesar M. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: 2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 17264634 Numbering: – Type: volume Value: 41 – Type: issue Value: 4 Titles: – TitleFull: Revista Peruana de Medicina Experimental y Salud Pública Type: main |
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