Las vesículas extracelulares circulantes modifican la diferenciación de células estromales mesenquimales y angiogénesis. Potencial uso en regeneración ósea.
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| Title: | Las vesículas extracelulares circulantes modifican la diferenciación de células estromales mesenquimales y angiogénesis. Potencial uso en regeneración ósea. |
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| Alternate Title: | Circulating extracellular vesicles affect mesenchymal stromal cell differentiation and angiogenesis. Potential use in bone regeneration. |
| Authors: | Oliva-Lozano, Sara1, Pulido-Escribano, Victoria1, Torrecillas-Baena, Bárbara1, Calañas-Continente, Alfonso1,2, Priego-Capote, Feliciano3,4, Camacho-Cardenosa, Marta1, Casado-Díaz, Antonio1,2,4 bb1cadia@uco.es |
| Source: | Journal of Osteoporosis & Mineral Metabolism / Revista de Osteoporosis y Metabolismo Mineral (Spanish edition). Jan-Mar2025, Vol. 17 Issue 1, p19-30. 24p. |
| Subjects: | FATTY acid-binding proteins, CORD blood, STROMAL cells, YOUNG adults, UMBILICAL veins |
| Abstract (English): | Introduction: the use of extracellular vesicles (EVs) has a high potential in regenerative medicine. Although mainly those derived from mesenchymal stromal cells (MSC) have been studied, circulating EVs from umbilical cord blood (UCBEV) or from healthy young adults (peEV) also contain factors that can favor tissue regeneration. This study evaluates the effect of UCBEV and peEV on MSC differentiation to osteoblasts and adipocytes, and endothelial cell angiogenesis. Material and methods: MSC cultures were treated with UCBEV and peEV during differentiation into osteoblasts or adipocytes. The expression of osteoblastic or adipogenic genes was studied. Mineralization and lipid droplet formation were quantified. Umbilical cord vein endothelial cells (HUVEC) were evaluated in angiogenesis assays. Results: UCBEV and peEV did not affect MSC viability, but peEV increased HUVEC viability. In osteoblasts, collagen type I alpha 1 (COL1A1) expression was increased by peEV, but mineralization was not affected. In adipocytes, adipose triglyceride lipase (ATGL) and fatty acid-binding protein 4 (FABP4) expression was inhibited, and lipid droplet formation was decreased with both types of EV. In HUVEC, UCBEV and peEV induced angiogenesis. Conclusion: the results suggest that both types of EVs, from abundant sources, without major ethical issues and easy to isolate, have high potential in regenerative medicine applied to bone, inhibiting bone marrow adiposity and favoring angiogenesis. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Introducción: el uso de vesículas extracelulares (VE) tiene un alto potencial en medicina regenerativa. Aunque principalmente se han estudiado las derivadas de células estromales mesenquimales (MSC), las VE circulantes de sangre de cordón umbilical (veCU) o de adultos jóvenes sanos (veAD), también contienen factores que pueden favorecer la regeneración tisular. Este estudio evalúa el efecto de veCU y veAD sobre la diferenciación de MSC a osteoblastos y adipocitos, y la angiogénesis de células endoteliales. Material y métodos: las VE fueron aisladas por cromatografía de exclusión de tamaño, caracterizadas y cuantificadas. Cultivos de MSC fueron tratados con veCU y veAD durante la diferenciación a osteoblastos o adipocitos. En ellas se estudió la expresión de genes osteoblásticos o adipogénicos, la mineralización y la formación de vesículas de grasa. Fueron evaluadas células endoteliales de vena de cordón umbilical (HUVEC) en ensayos de angiogénesis. Resultados: VeCU y veAD no afectaron a la viabilidad de las MSC, pero veAD aumentó la de HUVEC. En osteoblastos, aumentó la expresión de colágeno alfa-1 tipo I (COL1A1) con veAD, pero la mineralización no se afectó. En adipocitos se inhibió la expresión de adipo-triglicérido-lipasa (ATGL) y la proteína 4 de unión a ácido graso (FABP4), y disminuyó la formación de vesículas de grasa con ambos tipos de VE. En HUVEC, veCU y veAD indujeron la angiogénesis. Conclusión: los resultados sugieren que ambos tipos de VE, procedentes de fuentes abundantes, sin aspectos éticos importantes y fáciles de aislar, tienen un alto potencial en medicina regenerativa aplicada al hueso, inhibiendo la adiposidad de la médula ósea y favoreciendo la angiogénesis. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Osteoporosis & Mineral Metabolism / Revista de Osteoporosis y Metabolismo Mineral (Spanish edition) is the property of Ibanez y Plaza and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
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| Header | DbId: lth DbLabel: MedicLatina An: 184478888 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Las vesículas extracelulares circulantes modifican la diferenciación de células estromales mesenquimales y angiogénesis. Potencial uso en regeneración ósea. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: Circulating extracellular vesicles affect mesenchymal stromal cell differentiation and angiogenesis. Potential use in bone regeneration. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Oliva-Lozano%2C+Sara%22">Oliva-Lozano, Sara</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Pulido-Escribano%2C+Victoria%22">Pulido-Escribano, Victoria</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Torrecillas-Baena%2C+Bárbara%22">Torrecillas-Baena, Bárbara</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Calañas-Continente%2C+Alfonso%22">Calañas-Continente, Alfonso</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Priego-Capote%2C+Feliciano%22">Priego-Capote, Feliciano</searchLink><relatesTo>3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Camacho-Cardenosa%2C+Marta%22">Camacho-Cardenosa, Marta</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Casado-Díaz%2C+Antonio%22">Casado-Díaz, Antonio</searchLink><relatesTo>1,2,4</relatesTo><i> bb1cadia@uco.es</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Osteoporosis+%26+Mineral+Metabolism+%2F+Revista+de+Osteoporosis+y+Metabolismo+Mineral+%28Spanish+edition%29%22">Journal of Osteoporosis & Mineral Metabolism / Revista de Osteoporosis y Metabolismo Mineral (Spanish edition)</searchLink>. Jan-Mar2025, Vol. 17 Issue 1, p19-30. 24p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22FATTY+acid-binding+proteins%22">FATTY acid-binding proteins</searchLink><br /><searchLink fieldCode="DE" term="%22CORD+blood%22">CORD blood</searchLink><br /><searchLink fieldCode="DE" term="%22STROMAL+cells%22">STROMAL cells</searchLink><br /><searchLink fieldCode="DE" term="%22YOUNG+adults%22">YOUNG adults</searchLink><br /><searchLink fieldCode="DE" term="%22UMBILICAL+veins%22">UMBILICAL veins</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: Introduction: the use of extracellular vesicles (EVs) has a high potential in regenerative medicine. Although mainly those derived from mesenchymal stromal cells (MSC) have been studied, circulating EVs from umbilical cord blood (UCBEV) or from healthy young adults (peEV) also contain factors that can favor tissue regeneration. This study evaluates the effect of UCBEV and peEV on MSC differentiation to osteoblasts and adipocytes, and endothelial cell angiogenesis. Material and methods: MSC cultures were treated with UCBEV and peEV during differentiation into osteoblasts or adipocytes. The expression of osteoblastic or adipogenic genes was studied. Mineralization and lipid droplet formation were quantified. Umbilical cord vein endothelial cells (HUVEC) were evaluated in angiogenesis assays. Results: UCBEV and peEV did not affect MSC viability, but peEV increased HUVEC viability. In osteoblasts, collagen type I alpha 1 (COL1A1) expression was increased by peEV, but mineralization was not affected. In adipocytes, adipose triglyceride lipase (ATGL) and fatty acid-binding protein 4 (FABP4) expression was inhibited, and lipid droplet formation was decreased with both types of EV. In HUVEC, UCBEV and peEV induced angiogenesis. Conclusion: the results suggest that both types of EVs, from abundant sources, without major ethical issues and easy to isolate, have high potential in regenerative medicine applied to bone, inhibiting bone marrow adiposity and favoring angiogenesis. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Introducción: el uso de vesículas extracelulares (VE) tiene un alto potencial en medicina regenerativa. Aunque principalmente se han estudiado las derivadas de células estromales mesenquimales (MSC), las VE circulantes de sangre de cordón umbilical (veCU) o de adultos jóvenes sanos (veAD), también contienen factores que pueden favorecer la regeneración tisular. Este estudio evalúa el efecto de veCU y veAD sobre la diferenciación de MSC a osteoblastos y adipocitos, y la angiogénesis de células endoteliales. Material y métodos: las VE fueron aisladas por cromatografía de exclusión de tamaño, caracterizadas y cuantificadas. Cultivos de MSC fueron tratados con veCU y veAD durante la diferenciación a osteoblastos o adipocitos. En ellas se estudió la expresión de genes osteoblásticos o adipogénicos, la mineralización y la formación de vesículas de grasa. Fueron evaluadas células endoteliales de vena de cordón umbilical (HUVEC) en ensayos de angiogénesis. Resultados: VeCU y veAD no afectaron a la viabilidad de las MSC, pero veAD aumentó la de HUVEC. En osteoblastos, aumentó la expresión de colágeno alfa-1 tipo I (COL1A1) con veAD, pero la mineralización no se afectó. En adipocitos se inhibió la expresión de adipo-triglicérido-lipasa (ATGL) y la proteína 4 de unión a ácido graso (FABP4), y disminuyó la formación de vesículas de grasa con ambos tipos de VE. En HUVEC, veCU y veAD indujeron la angiogénesis. Conclusión: los resultados sugieren que ambos tipos de VE, procedentes de fuentes abundantes, sin aspectos éticos importantes y fáciles de aislar, tienen un alto potencial en medicina regenerativa aplicada al hueso, inhibiendo la adiposidad de la médula ósea y favoreciendo la angiogénesis. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Osteoporosis & Mineral Metabolism / Revista de Osteoporosis y Metabolismo Mineral (Spanish edition) is the property of Ibanez y Plaza and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.20960/RevOsteoporosMetabMiner.00066 Languages: – Code: mul Text: Multiple languages PhysicalDescription: Pagination: PageCount: 24 StartPage: 19 Subjects: – SubjectFull: FATTY acid-binding proteins Type: general – SubjectFull: CORD blood Type: general – SubjectFull: STROMAL cells Type: general – SubjectFull: YOUNG adults Type: general – SubjectFull: UMBILICAL veins Type: general Titles: – TitleFull: Las vesículas extracelulares circulantes modifican la diferenciación de células estromales mesenquimales y angiogénesis. Potencial uso en regeneración ósea. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Oliva-Lozano, Sara – PersonEntity: Name: NameFull: Pulido-Escribano, Victoria – PersonEntity: Name: NameFull: Torrecillas-Baena, Bárbara – PersonEntity: Name: NameFull: Calañas-Continente, Alfonso – PersonEntity: Name: NameFull: Priego-Capote, Feliciano – PersonEntity: Name: NameFull: Camacho-Cardenosa, Marta – PersonEntity: Name: NameFull: Casado-Díaz, Antonio IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan-Mar2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 1889836X Numbering: – Type: volume Value: 17 – Type: issue Value: 1 Titles: – TitleFull: Journal of Osteoporosis & Mineral Metabolism / Revista de Osteoporosis y Metabolismo Mineral (Spanish edition) Type: main |
| ResultId | 1 |
