Validación de muestras, técnicas y estrategias de diagnóstico de virus emergentes (experiencia con COVID-19).
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| Title: | Validación de muestras, técnicas y estrategias de diagnóstico de virus emergentes (experiencia con COVID-19). |
|---|---|
| Alternate Title: | Validation of samples, techniques, and diagnostic strategies for emerging viruses (experience in COVID-19). |
| Authors: | Shoji, Kenji F.1 kenjishoji@gmail.com, Aleman Castillo, Roxana2 roxana.aleman@uajms.edu.bo, Aguirre-Villegas, Horacio Andres3 aguirreville@wisc.edu, Arce Cardozo, Rodrigo K.4,5 rodrigo.arce@umu.se |
| Source: | Gaceta Médica Boliviana. ene-jun2025, Vol. 48 Issue 1, p7-13. 7p. |
| Subjects: | COVID-19, LOOP-mediated isothermal amplification, NASOPHARYNX diseases, REVERSE transcriptase polymerase chain reaction, DIAGNOSIS, EMERGING infectious diseases, SALIVA analysis, SENSITIVITY & specificity (Statistics) |
| Abstract (English): | Objectives: currently, the standard qRT-PCR virus detection technique requires trained personnel, specialized equipment and relatively expensive reagents. The loop-mediated isothermal amplification technique, LAMP, has reemerged as a promising alternative previously and during the COVID-19 pandemic and its study has potential for the control of emerging infectious diseases. Methods: in this work, we present a comparative study carried out by the SEDES of Tarija in October 2020 where samples from volunteer patients were compared using RTPCR and LAMP in nasopharyngeal swab and saliva samples. Results: LAMP demonstrated 100% concordance with PCR in nasopharyngeal RNA samples, and 93.5% concordance with saliva samples. Conclusions: the rapid and practical diagnosis of emerging infectious diseases such as COVID-19 is crucial to establish effective control strategies that minimize the economic impact and in turn maximize protection for its citizens. Our results validate the sensitivity of LAMP and the use of saliva samples as a less invasive alternative. This work confirms the validity of the technique and its easy implementation in rural areas with limited resources for the control of emerging viral diseases. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Objetivos: actualmente, la técnica estándar de detección de virus qRT-PCR, requiere personal capacitado, equipos especializados y reactivos relativamente costosos. La técnica de amplificación isotérmica mediada por bucle, LAMP, ha resurgido como una alternativa prometedora previamente y durante la pandemia de COVID-19 y su estudio tiene potencial para el control de enfermedades infecciosas emergentes. Métodos: en este trabajo, presentamos un estudio comparativo realizado por el SEDES de Tarija en octubre del 2020 donde se comparó muestras de pacientes voluntarios utilizando RT-PCR y LAMP en muestras de hisopados nasofaríngeos y de saliva. Resultados: LAMP demostró una concordancia del 100% con PCR en muestras de ARN nasofaríngeo, y un 93,5% de concordancia con muestras de saliva. Conclusiones: el diagnóstico rápido y práctico de enfermedades infecciosas emergentes como el COVID-19 es crucial para establecer estrategias de control eficaces que minimicen el impacto económico y que a su vez maximicen la protección sobre sus ciudadanos. Nuestros resultados validan la sensibilidad de LAMP y el uso de muestras de saliva como alternativa menos invasiva. Este trabajo confirma la validez de la técnica y su fácil implementación en áreas rurales con recursos limitados para el control de enfermedades virales emergentes. [ABSTRACT FROM AUTHOR] |
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| Database: | MedicLatina |
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