Genomic fragment detection and infectivity evaluation of rotaviruses isolated from wastewater used for irrigation in western Bogotá, D. C.

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Bibliographic Details
Title: Genomic fragment detection and infectivity evaluation of rotaviruses isolated from wastewater used for irrigation in western Bogotá, D. C.
Alternate Title: Detección de fragmentos genómicos y evaluación de la capacidad infecciosa de rotavirus aislados de aguas residuales utilizadas para riego en el occidente de Bogotá, D. C.
Authors: Jordán, José Seir1, Guerrero, Carlos Arturo1 caguerrerof@unal.edu.co
Source: Biomédica: Revista del Instituto Nacional de Salud. 2025 Special Issue, Vol. 45, p30-40. 11p.
Subjects: ROTAVIRUSES, ENVIRONMENTAL health, IRRIGATION, EPIDEMIOLOGY, SEWAGE, MICROBIAL virulence, MOLECULAR diagnosis
Geographic Terms: BOGOTA (Colombia), COLOMBIA
Abstract (English): Introduction. Enteric viruses significantly impact morbidity, mortality, and healthcare. Transmission through wastewater is favoured in highly contaminated areas due to inadequate treatment. Objective. To determine the number of rotaviruses and their infectious capacity from wastewater samples used for irrigation in the western part of Bogotá. Materials and methods. Concentrations of group A rotavirus were monitored in wastewater using molecular methods. The infectivity of rotaviruses was evaluated in a mouse intestinal villi model. We assessed the feasibility of applying this approach for environmental health surveillance in Colombia, considering findings reported by other authors. Results. The research focused on the La Ramada irrigation network in the western part of Bogotá, specifically the Canal San José. We analysed eighteen wastewater samples using qRT-PCR and detected group A rotavirus in twelve of them. The positive samples contained infectious rotavirus, as confirmed through the mouse villi model. Conclusion. This study shows that contamination by group A rotavirus is frequent in wastewaters from the Canal San José in the La Ramada irrigation network in the western part of Bogotá and reveals high concentrations of rotavirus. The results suggest that villi from mouse intestines serve as a reliable model for isolating rotavirus from wastewaters. These findings provide a new approach for environmental health surveillance in Colombia, based on molecular epidemiology for waters highly contaminated with human enteric viruses. [ABSTRACT FROM AUTHOR]
Abstract (Spanish): Introducción. Los virus entéricos impactan significativamente la morbilidad, la mortalidad y los costos de atención médica. Su transmisión por medio de aguas residuales se potencia en zonas muy contaminadas debido al tratamiento deficiente de dichas aguas. Objetivo. Determinar la cantidad de rotavirus y su capacidad infecciosa a partir de muestras de aguas residuales utilizadas para riego en el occidente de Bogotá. Materiales y métodos. Se monitorearon concentraciones de rotavirus de tipo A en aguas residuales mediante métodos moleculares. La capacidad infecciosa de los rotavirus se evaluó en un modelo de vellosidades intestinales de ratón. Se analizó la posibilidad de utilizar este enfoque para la vigilancia de la salud ambiental en Colombia, tomando como referencia resultados similares reportados por otros autores. Resultados. La investigación incluyó la red de riego La Ramada, al occidente de Bogotá, particularmente el Canal San José. Dieciocho muestras de aguas residuales fueron analizadas mediante qRT-PCR. Se detectaron rotavirus de tipo A en doce de ellas. Las doce muestras positivas contenían rotavirus infecciosos, como se comprobó en el modelo de vellosidades intestinales de ratón. Conclusión. Los resultados muestran que la contaminación por rotavirus de tipo A es frecuente en aguas residuales del Canal San José de la red de riego La Ramada, al occidente de Bogotá, y revela grandes concentraciones del virus. Los resultados sugieren que las vellosidades del intestino de ratón sirven como modelo fiable para el aislamiento de rotavirus presentes en aguas residuales. Estos hallazgos proporcionan un nuevo enfoque de vigilancia de la salud ambiental en Colombia basado en epidemiología molecular para aguas muy contaminadas con virus entéricos humanos. [ABSTRACT FROM AUTHOR]
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Database: MedicLatina
Description
Abstract:Introduction. Enteric viruses significantly impact morbidity, mortality, and healthcare. Transmission through wastewater is favoured in highly contaminated areas due to inadequate treatment. Objective. To determine the number of rotaviruses and their infectious capacity from wastewater samples used for irrigation in the western part of Bogotá. Materials and methods. Concentrations of group A rotavirus were monitored in wastewater using molecular methods. The infectivity of rotaviruses was evaluated in a mouse intestinal villi model. We assessed the feasibility of applying this approach for environmental health surveillance in Colombia, considering findings reported by other authors. Results. The research focused on the La Ramada irrigation network in the western part of Bogotá, specifically the Canal San José. We analysed eighteen wastewater samples using qRT-PCR and detected group A rotavirus in twelve of them. The positive samples contained infectious rotavirus, as confirmed through the mouse villi model. Conclusion. This study shows that contamination by group A rotavirus is frequent in wastewaters from the Canal San José in the La Ramada irrigation network in the western part of Bogotá and reveals high concentrations of rotavirus. The results suggest that villi from mouse intestines serve as a reliable model for isolating rotavirus from wastewaters. These findings provide a new approach for environmental health surveillance in Colombia, based on molecular epidemiology for waters highly contaminated with human enteric viruses. [ABSTRACT FROM AUTHOR]
ISSN:01204157
DOI:10.7705/biomedica.7935