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Genomic fragment detection and infectivity evaluation of rotaviruses isolated from wastewater used for irrigation in western Bogotá, D. C.

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Title: Genomic fragment detection and infectivity evaluation of rotaviruses isolated from wastewater used for irrigation in western Bogotá, D. C.
Alternate Title: Detección de fragmentos genómicos y evaluación de la capacidad infecciosa de rotavirus aislados de aguas residuales utilizadas para riego en el occidente de Bogotá, D. C.
Authors: Jordán, José Seir1, Guerrero, Carlos Arturo1 caguerrerof@unal.edu.co
Source: Biomédica: Revista del Instituto Nacional de Salud. 2025 Special Issue, Vol. 45, p30-40. 11p.
Subjects: ROTAVIRUSES, ENVIRONMENTAL health, IRRIGATION, EPIDEMIOLOGY, SEWAGE, MICROBIAL virulence, MOLECULAR diagnosis
Geographic Terms: BOGOTA (Colombia), COLOMBIA
Abstract (English): Introduction. Enteric viruses significantly impact morbidity, mortality, and healthcare. Transmission through wastewater is favoured in highly contaminated areas due to inadequate treatment. Objective. To determine the number of rotaviruses and their infectious capacity from wastewater samples used for irrigation in the western part of Bogotá. Materials and methods. Concentrations of group A rotavirus were monitored in wastewater using molecular methods. The infectivity of rotaviruses was evaluated in a mouse intestinal villi model. We assessed the feasibility of applying this approach for environmental health surveillance in Colombia, considering findings reported by other authors. Results. The research focused on the La Ramada irrigation network in the western part of Bogotá, specifically the Canal San José. We analysed eighteen wastewater samples using qRT-PCR and detected group A rotavirus in twelve of them. The positive samples contained infectious rotavirus, as confirmed through the mouse villi model. Conclusion. This study shows that contamination by group A rotavirus is frequent in wastewaters from the Canal San José in the La Ramada irrigation network in the western part of Bogotá and reveals high concentrations of rotavirus. The results suggest that villi from mouse intestines serve as a reliable model for isolating rotavirus from wastewaters. These findings provide a new approach for environmental health surveillance in Colombia, based on molecular epidemiology for waters highly contaminated with human enteric viruses. [ABSTRACT FROM AUTHOR]
Abstract (Spanish): Introducción. Los virus entéricos impactan significativamente la morbilidad, la mortalidad y los costos de atención médica. Su transmisión por medio de aguas residuales se potencia en zonas muy contaminadas debido al tratamiento deficiente de dichas aguas. Objetivo. Determinar la cantidad de rotavirus y su capacidad infecciosa a partir de muestras de aguas residuales utilizadas para riego en el occidente de Bogotá. Materiales y métodos. Se monitorearon concentraciones de rotavirus de tipo A en aguas residuales mediante métodos moleculares. La capacidad infecciosa de los rotavirus se evaluó en un modelo de vellosidades intestinales de ratón. Se analizó la posibilidad de utilizar este enfoque para la vigilancia de la salud ambiental en Colombia, tomando como referencia resultados similares reportados por otros autores. Resultados. La investigación incluyó la red de riego La Ramada, al occidente de Bogotá, particularmente el Canal San José. Dieciocho muestras de aguas residuales fueron analizadas mediante qRT-PCR. Se detectaron rotavirus de tipo A en doce de ellas. Las doce muestras positivas contenían rotavirus infecciosos, como se comprobó en el modelo de vellosidades intestinales de ratón. Conclusión. Los resultados muestran que la contaminación por rotavirus de tipo A es frecuente en aguas residuales del Canal San José de la red de riego La Ramada, al occidente de Bogotá, y revela grandes concentraciones del virus. Los resultados sugieren que las vellosidades del intestino de ratón sirven como modelo fiable para el aislamiento de rotavirus presentes en aguas residuales. Estos hallazgos proporcionan un nuevo enfoque de vigilancia de la salud ambiental en Colombia basado en epidemiología molecular para aguas muy contaminadas con virus entéricos humanos. [ABSTRACT FROM AUTHOR]
Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Genomic fragment detection and infectivity evaluation of rotaviruses isolated from wastewater used for irrigation in western Bogotá, D. C.
– Name: TitleAlt
  Label: Alternate Title
  Group: TiAlt
  Data: Detección de fragmentos genómicos y evaluación de la capacidad infecciosa de rotavirus aislados de aguas residuales utilizadas para riego en el occidente de Bogotá, D. C.
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  Data: <searchLink fieldCode="AR" term="%22Jordán%2C+José+Seir%22">Jordán, José Seir</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Guerrero%2C+Carlos+Arturo%22">Guerrero, Carlos Arturo</searchLink><relatesTo>1</relatesTo><i> caguerrerof@unal.edu.co</i>
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  Data: <searchLink fieldCode="JN" term="%22Biomédica%3A+Revista+del+Instituto+Nacional+de+Salud%22">Biomédica: Revista del Instituto Nacional de Salud</searchLink>. 2025 Special Issue, Vol. 45, p30-40. 11p.
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  Data: <searchLink fieldCode="DE" term="%22ROTAVIRUSES%22">ROTAVIRUSES</searchLink><br /><searchLink fieldCode="DE" term="%22ENVIRONMENTAL+health%22">ENVIRONMENTAL health</searchLink><br /><searchLink fieldCode="DE" term="%22IRRIGATION%22">IRRIGATION</searchLink><br /><searchLink fieldCode="DE" term="%22EPIDEMIOLOGY%22">EPIDEMIOLOGY</searchLink><br /><searchLink fieldCode="DE" term="%22SEWAGE%22">SEWAGE</searchLink><br /><searchLink fieldCode="DE" term="%22MICROBIAL+virulence%22">MICROBIAL virulence</searchLink><br /><searchLink fieldCode="DE" term="%22MOLECULAR+diagnosis%22">MOLECULAR diagnosis</searchLink>
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  Data: <searchLink fieldCode="DE" term="%22BOGOTA+%28Colombia%29%22">BOGOTA (Colombia)</searchLink><br /><searchLink fieldCode="DE" term="%22COLOMBIA%22">COLOMBIA</searchLink>
– Name: Abstract
  Label: Abstract (English)
  Group: Ab
  Data: Introduction. Enteric viruses significantly impact morbidity, mortality, and healthcare. Transmission through wastewater is favoured in highly contaminated areas due to inadequate treatment. Objective. To determine the number of rotaviruses and their infectious capacity from wastewater samples used for irrigation in the western part of Bogotá. Materials and methods. Concentrations of group A rotavirus were monitored in wastewater using molecular methods. The infectivity of rotaviruses was evaluated in a mouse intestinal villi model. We assessed the feasibility of applying this approach for environmental health surveillance in Colombia, considering findings reported by other authors. Results. The research focused on the La Ramada irrigation network in the western part of Bogotá, specifically the Canal San José. We analysed eighteen wastewater samples using qRT-PCR and detected group A rotavirus in twelve of them. The positive samples contained infectious rotavirus, as confirmed through the mouse villi model. Conclusion. This study shows that contamination by group A rotavirus is frequent in wastewaters from the Canal San José in the La Ramada irrigation network in the western part of Bogotá and reveals high concentrations of rotavirus. The results suggest that villi from mouse intestines serve as a reliable model for isolating rotavirus from wastewaters. These findings provide a new approach for environmental health surveillance in Colombia, based on molecular epidemiology for waters highly contaminated with human enteric viruses. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label: Abstract (Spanish)
  Group: Ab
  Data: Introducción. Los virus entéricos impactan significativamente la morbilidad, la mortalidad y los costos de atención médica. Su transmisión por medio de aguas residuales se potencia en zonas muy contaminadas debido al tratamiento deficiente de dichas aguas. Objetivo. Determinar la cantidad de rotavirus y su capacidad infecciosa a partir de muestras de aguas residuales utilizadas para riego en el occidente de Bogotá. Materiales y métodos. Se monitorearon concentraciones de rotavirus de tipo A en aguas residuales mediante métodos moleculares. La capacidad infecciosa de los rotavirus se evaluó en un modelo de vellosidades intestinales de ratón. Se analizó la posibilidad de utilizar este enfoque para la vigilancia de la salud ambiental en Colombia, tomando como referencia resultados similares reportados por otros autores. Resultados. La investigación incluyó la red de riego La Ramada, al occidente de Bogotá, particularmente el Canal San José. Dieciocho muestras de aguas residuales fueron analizadas mediante qRT-PCR. Se detectaron rotavirus de tipo A en doce de ellas. Las doce muestras positivas contenían rotavirus infecciosos, como se comprobó en el modelo de vellosidades intestinales de ratón. Conclusión. Los resultados muestran que la contaminación por rotavirus de tipo A es frecuente en aguas residuales del Canal San José de la red de riego La Ramada, al occidente de Bogotá, y revela grandes concentraciones del virus. Los resultados sugieren que las vellosidades del intestino de ratón sirven como modelo fiable para el aislamiento de rotavirus presentes en aguas residuales. Estos hallazgos proporcionan un nuevo enfoque de vigilancia de la salud ambiental en Colombia basado en epidemiología molecular para aguas muy contaminadas con virus entéricos humanos. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lth&AN=190594659
RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.7705/biomedica.7935
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      – Code: eng
        Text: English
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        PageCount: 11
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      – SubjectFull: ROTAVIRUSES
        Type: general
      – SubjectFull: ENVIRONMENTAL health
        Type: general
      – SubjectFull: IRRIGATION
        Type: general
      – SubjectFull: EPIDEMIOLOGY
        Type: general
      – SubjectFull: SEWAGE
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      – SubjectFull: MICROBIAL virulence
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      – SubjectFull: MOLECULAR diagnosis
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      – SubjectFull: BOGOTA (Colombia)
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      – SubjectFull: COLOMBIA
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      – TitleFull: Genomic fragment detection and infectivity evaluation of rotaviruses isolated from wastewater used for irrigation in western Bogotá, D. C.
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              Text: 2025 Special Issue
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