Revelando el papel del gen AGO4 frente al virus de la hoja blanca del arroz: de la transformación a la estructura proteica.
Saved in:
| Title: | Revelando el papel del gen AGO4 frente al virus de la hoja blanca del arroz: de la transformación a la estructura proteica. |
|---|---|
| Authors: | NAÑEZ, JHOJAN1 Varela.j.nanez@cgiar.org, VALDES, SANDRA1, CRUZ-GALLEGO, MARIBEL1,2, REBOLLEDO, CAMILA1,3, LORIEUX, MATHIAS3, ALVAREZ, MARIA FERNANDA1, CHAVARRIAGA, PAUL1 |
| Source: | Salud Uninorte. 2025 Special Issue, p1-2. 2p. |
| Subjects: | ARGONAUTE proteins, RICE diseases & pests, GENE expression profiling, CRISPRS, GENOME editing, PLANT viruses, PROTEIN models, PHENOTYPES |
| Abstract (English): | The article focuses on the role of the AGO4 gene in rice resistance to the rice hoja blanca virus (RHBV). Using CRISPR/Cas9 technology, mutations were produced in the AGO4 gene of the Fedearroz 2000 variety, resulting in 14 edited plants that showed increased susceptibility to the virus. Phenotypic evaluations and expression analysis of the AGO4 gene indicated that the edited lines exhibited profiles similar to those of a susceptible control. Additionally, modeling of the AGO4 protein structure revealed significant changes that support its function in resistance to RHBV. [Extracted from the article] |
| Abstract (Spanish): | Introducción: El virus de la hoja blanca del arroz (RHBV), transmitido por el insecto vector Tagosodes orizicolus, representa una amenaza significativa para el cultivo del arroz. Métodos: Utilizamos la tecnología CRISPR/Cas9 para producir mutaciones específicas en el gen AGO4 de Oryza sativa, utilizando la variedad Fedearroz 2000, con el objetivo de dilucidar la participación del gen en la resistencia a RHBV. Resultados: Obtuvimos 14 plantas editadas que presentaron deleciones de uno, dos y tres nucleótidos en la secuencia del exón 23 del gen AGO4. Las evaluaciones fenotípicas mostraron un aumento en la susceptibilidad a RHBV en las líneas editadas. Identificamos la presencia de RHBV en el tejido foliar de plantas infectadas mediante la amplificación de los genes de la nucleoproteína, NS3 y NS4 del virus. Mediante RT-qPCR analizamos los patrones de expresión del gen AGO4, lo cual mostró que, en las líneas editadas, los perfiles de expresión son similares al control susceptible. Además, el modelado de la estructura terciaria de la proteína AGO4 y su variante mutante demostró cambios en el dominio PIWI y la presencia de la tríada catalítica DDH, lo que confirma su papel en la mediación de la resistencia al RHBV. Conclusiones: Nuestro estudio revela la importancia funcional del gen AGO4 del arroz en la resistencia al RHBV. [ABSTRACT FROM AUTHOR] |
| Copyright of Salud Uninorte is the property of Fundacion Universidad del Norte and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
|---|---|
| Header | DbId: lth DbLabel: MedicLatina An: 191656110 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Revelando el papel del gen AGO4 frente al virus de la hoja blanca del arroz: de la transformación a la estructura proteica. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22NAÑEZ%2C+JHOJAN%22">NAÑEZ, JHOJAN</searchLink><relatesTo>1</relatesTo><i> Varela.j.nanez@cgiar.org</i><br /><searchLink fieldCode="AR" term="%22VALDES%2C+SANDRA%22">VALDES, SANDRA</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22CRUZ-GALLEGO%2C+MARIBEL%22">CRUZ-GALLEGO, MARIBEL</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22REBOLLEDO%2C+CAMILA%22">REBOLLEDO, CAMILA</searchLink><relatesTo>1,3</relatesTo><br /><searchLink fieldCode="AR" term="%22LORIEUX%2C+MATHIAS%22">LORIEUX, MATHIAS</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22ALVAREZ%2C+MARIA+FERNANDA%22">ALVAREZ, MARIA FERNANDA</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22CHAVARRIAGA%2C+PAUL%22">CHAVARRIAGA, PAUL</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Salud+Uninorte%22">Salud Uninorte</searchLink>. 2025 Special Issue, p1-2. 2p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22ARGONAUTE+proteins%22">ARGONAUTE proteins</searchLink><br /><searchLink fieldCode="DE" term="%22RICE+diseases+%26+pests%22">RICE diseases & pests</searchLink><br /><searchLink fieldCode="DE" term="%22GENE+expression+profiling%22">GENE expression profiling</searchLink><br /><searchLink fieldCode="DE" term="%22CRISPRS%22">CRISPRS</searchLink><br /><searchLink fieldCode="DE" term="%22GENOME+editing%22">GENOME editing</searchLink><br /><searchLink fieldCode="DE" term="%22PLANT+viruses%22">PLANT viruses</searchLink><br /><searchLink fieldCode="DE" term="%22PROTEIN+models%22">PROTEIN models</searchLink><br /><searchLink fieldCode="DE" term="%22PHENOTYPES%22">PHENOTYPES</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: The article focuses on the role of the AGO4 gene in rice resistance to the rice hoja blanca virus (RHBV). Using CRISPR/Cas9 technology, mutations were produced in the AGO4 gene of the Fedearroz 2000 variety, resulting in 14 edited plants that showed increased susceptibility to the virus. Phenotypic evaluations and expression analysis of the AGO4 gene indicated that the edited lines exhibited profiles similar to those of a susceptible control. Additionally, modeling of the AGO4 protein structure revealed significant changes that support its function in resistance to RHBV. [Extracted from the article] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Introducción: El virus de la hoja blanca del arroz (RHBV), transmitido por el insecto vector Tagosodes orizicolus, representa una amenaza significativa para el cultivo del arroz. Métodos: Utilizamos la tecnología CRISPR/Cas9 para producir mutaciones específicas en el gen AGO4 de Oryza sativa, utilizando la variedad Fedearroz 2000, con el objetivo de dilucidar la participación del gen en la resistencia a RHBV. Resultados: Obtuvimos 14 plantas editadas que presentaron deleciones de uno, dos y tres nucleótidos en la secuencia del exón 23 del gen AGO4. Las evaluaciones fenotípicas mostraron un aumento en la susceptibilidad a RHBV en las líneas editadas. Identificamos la presencia de RHBV en el tejido foliar de plantas infectadas mediante la amplificación de los genes de la nucleoproteína, NS3 y NS4 del virus. Mediante RT-qPCR analizamos los patrones de expresión del gen AGO4, lo cual mostró que, en las líneas editadas, los perfiles de expresión son similares al control susceptible. Además, el modelado de la estructura terciaria de la proteína AGO4 y su variante mutante demostró cambios en el dominio PIWI y la presencia de la tríada catalítica DDH, lo que confirma su papel en la mediación de la resistencia al RHBV. Conclusiones: Nuestro estudio revela la importancia funcional del gen AGO4 del arroz en la resistencia al RHBV. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Salud Uninorte is the property of Fundacion Universidad del Norte and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lth&AN=191656110 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.14482/sun.01.745.698 Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 2 StartPage: 1 Subjects: – SubjectFull: ARGONAUTE proteins Type: general – SubjectFull: RICE diseases & pests Type: general – SubjectFull: GENE expression profiling Type: general – SubjectFull: CRISPRS Type: general – SubjectFull: GENOME editing Type: general – SubjectFull: PLANT viruses Type: general – SubjectFull: PROTEIN models Type: general – SubjectFull: PHENOTYPES Type: general Titles: – TitleFull: Revelando el papel del gen AGO4 frente al virus de la hoja blanca del arroz: de la transformación a la estructura proteica. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: NAÑEZ, JHOJAN – PersonEntity: Name: NameFull: VALDES, SANDRA – PersonEntity: Name: NameFull: CRUZ-GALLEGO, MARIBEL – PersonEntity: Name: NameFull: REBOLLEDO, CAMILA – PersonEntity: Name: NameFull: LORIEUX, MATHIAS – PersonEntity: Name: NameFull: ALVAREZ, MARIA FERNANDA – PersonEntity: Name: NameFull: CHAVARRIAGA, PAUL IsPartOfRelationships: – BibEntity: Dates: – D: 02 M: 09 Text: 2025 Special Issue Type: published Y: 2025 Identifiers: – Type: issn-print Value: 01205552 Titles: – TitleFull: Salud Uninorte Type: main |
| ResultId | 1 |
