Caracterización del viroma de Spodoptera frugiperda (biotipo maíz) mediante RNA-seq para la búsqueda de agentes con potencial en biocontrol.
Saved in:
| Title: | Caracterización del viroma de Spodoptera frugiperda (biotipo maíz) mediante RNA-seq para la búsqueda de agentes con potencial en biocontrol. |
|---|---|
| Authors: | RIVERA, NATALIA LÓPEZ1, YEPES-ACEVEDO, ANNY J.1, MONTOYA-RUIZ, CAROLINA2, SALDAMANDO, CLARA1, HERRERA, CLAUDIA XIMENA MORENO3 cisaldam@unal.edu.co |
| Source: | Salud Uninorte. 2025 Special Issue, p239-240. 2p. |
| Subjects: | FALL armyworm, BIOLOGICAL pest control agents, VIRUS diversity, BACULOVIRUSES, INSECTICIDE resistance, BUNYAVIRUSES, RNA sequencing |
| Geographic Terms: | COLOMBIA |
| Abstract (English): | The article focuses on the characterization of the virome of Spodoptera frugiperda (maize biotype) using RNA-seq, with the aim of identifying viruses that could be used in biocontrol. This pest, which affects crops such as maize and cotton, is showing increasing resistance to insecticides, highlighting the need for sustainable control strategies. Larvae were collected in Envigado, Colombia, and sequencing was performed to detect viral sequences from the families Phenuiviridae, Baculoviridae, and Belpaoviridae. The preliminary findings represent a step forward in the understanding of the virome of this pest in Colombia and suggest new opportunities for the development of biocontrol methods. [Extracted from the article] |
| Abstract (Spanish): | Introducción: Spodoptera frugiperda es una plaga devastadora que afecta cultivos como maíz, algodón, sorgo y caña de azúcar, generando pérdidas millonarias a nivel mundial. El biotipo maíz presenta amplia distribución y creciente resistencia a insecticidas, lo que resalta la necesidad de estrategias de control sostenibles. Entre ellas, los virus asociados al insecto representan una alternativa prometedora, aunque en Colombia el conocimiento sobre su viroma es limitado. El objetivo de este estudio fue caracterizar el viroma de larvas de S. frugiperda (biotipo maíz) para identificar virus con potencial en biocontrol. Métodos: Se recolectaron tres larvas en instar sexto que se alimentaban de plantas de maíz diferentes en Envigado (Antioquia); del cuerpo completo de las larvas se extrajo y purificó RNA, y se realizó secuenciación Illumina NovaSeq 6000 (paired-end). El análisis bioinformático incluyó control de calidad (FastQC), limpieza de lecturas (Trimmomatic), ensamblaje (rnaSPAdes) e identificación de virus mediante BLASTx contra la base de datos viral de NCBI. Resultados: Se detectaron secuencias virales correspondientes a las familias Phenuiviridae, Baculoviridae y Belpaoviridae. Actualmente, se avanza en la clasificación a nivel de género y en análisis filogenéticos. Conclusiones: Estos resultados preliminares constituyen uno de los primeros acercamientos al viroma de S. frugiperda en Colombia y abren nuevas perspectivas para el desarrollo de estrategias de biocontrol para esta plaga. [ABSTRACT FROM AUTHOR] |
| Copyright of Salud Uninorte is the property of Fundacion Universidad del Norte and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
|---|---|
| Header | DbId: lth DbLabel: MedicLatina An: 191656228 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Caracterización del viroma de Spodoptera frugiperda (biotipo maíz) mediante RNA-seq para la búsqueda de agentes con potencial en biocontrol. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22RIVERA%2C+NATALIA+LÓPEZ%22">RIVERA, NATALIA LÓPEZ</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22YEPES-ACEVEDO%2C+ANNY+J%2E%22">YEPES-ACEVEDO, ANNY J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22MONTOYA-RUIZ%2C+CAROLINA%22">MONTOYA-RUIZ, CAROLINA</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22SALDAMANDO%2C+CLARA%22">SALDAMANDO, CLARA</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22HERRERA%2C+CLAUDIA+XIMENA+MORENO%22">HERRERA, CLAUDIA XIMENA MORENO</searchLink><relatesTo>3</relatesTo><i> cisaldam@unal.edu.co</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Salud+Uninorte%22">Salud Uninorte</searchLink>. 2025 Special Issue, p239-240. 2p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22FALL+armyworm%22">FALL armyworm</searchLink><br /><searchLink fieldCode="DE" term="%22BIOLOGICAL+pest+control+agents%22">BIOLOGICAL pest control agents</searchLink><br /><searchLink fieldCode="DE" term="%22VIRUS+diversity%22">VIRUS diversity</searchLink><br /><searchLink fieldCode="DE" term="%22BACULOVIRUSES%22">BACULOVIRUSES</searchLink><br /><searchLink fieldCode="DE" term="%22INSECTICIDE+resistance%22">INSECTICIDE resistance</searchLink><br /><searchLink fieldCode="DE" term="%22BUNYAVIRUSES%22">BUNYAVIRUSES</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+sequencing%22">RNA sequencing</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22COLOMBIA%22">COLOMBIA</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: The article focuses on the characterization of the virome of Spodoptera frugiperda (maize biotype) using RNA-seq, with the aim of identifying viruses that could be used in biocontrol. This pest, which affects crops such as maize and cotton, is showing increasing resistance to insecticides, highlighting the need for sustainable control strategies. Larvae were collected in Envigado, Colombia, and sequencing was performed to detect viral sequences from the families Phenuiviridae, Baculoviridae, and Belpaoviridae. The preliminary findings represent a step forward in the understanding of the virome of this pest in Colombia and suggest new opportunities for the development of biocontrol methods. [Extracted from the article] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Introducción: Spodoptera frugiperda es una plaga devastadora que afecta cultivos como maíz, algodón, sorgo y caña de azúcar, generando pérdidas millonarias a nivel mundial. El biotipo maíz presenta amplia distribución y creciente resistencia a insecticidas, lo que resalta la necesidad de estrategias de control sostenibles. Entre ellas, los virus asociados al insecto representan una alternativa prometedora, aunque en Colombia el conocimiento sobre su viroma es limitado. El objetivo de este estudio fue caracterizar el viroma de larvas de S. frugiperda (biotipo maíz) para identificar virus con potencial en biocontrol. Métodos: Se recolectaron tres larvas en instar sexto que se alimentaban de plantas de maíz diferentes en Envigado (Antioquia); del cuerpo completo de las larvas se extrajo y purificó RNA, y se realizó secuenciación Illumina NovaSeq 6000 (paired-end). El análisis bioinformático incluyó control de calidad (FastQC), limpieza de lecturas (Trimmomatic), ensamblaje (rnaSPAdes) e identificación de virus mediante BLASTx contra la base de datos viral de NCBI. Resultados: Se detectaron secuencias virales correspondientes a las familias Phenuiviridae, Baculoviridae y Belpaoviridae. Actualmente, se avanza en la clasificación a nivel de género y en análisis filogenéticos. Conclusiones: Estos resultados preliminares constituyen uno de los primeros acercamientos al viroma de S. frugiperda en Colombia y abren nuevas perspectivas para el desarrollo de estrategias de biocontrol para esta plaga. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Salud Uninorte is the property of Fundacion Universidad del Norte and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lth&AN=191656228 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.14482/sun.01.918.624 Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 2 StartPage: 239 Subjects: – SubjectFull: FALL armyworm Type: general – SubjectFull: BIOLOGICAL pest control agents Type: general – SubjectFull: VIRUS diversity Type: general – SubjectFull: BACULOVIRUSES Type: general – SubjectFull: INSECTICIDE resistance Type: general – SubjectFull: BUNYAVIRUSES Type: general – SubjectFull: RNA sequencing Type: general – SubjectFull: COLOMBIA Type: general Titles: – TitleFull: Caracterización del viroma de Spodoptera frugiperda (biotipo maíz) mediante RNA-seq para la búsqueda de agentes con potencial en biocontrol. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: RIVERA, NATALIA LÓPEZ – PersonEntity: Name: NameFull: YEPES-ACEVEDO, ANNY J. – PersonEntity: Name: NameFull: MONTOYA-RUIZ, CAROLINA – PersonEntity: Name: NameFull: SALDAMANDO, CLARA – PersonEntity: Name: NameFull: HERRERA, CLAUDIA XIMENA MORENO IsPartOfRelationships: – BibEntity: Dates: – D: 02 M: 09 Text: 2025 Special Issue Type: published Y: 2025 Identifiers: – Type: issn-print Value: 01205552 Titles: – TitleFull: Salud Uninorte Type: main |
| ResultId | 1 |
