Antagonistic activity of native Streptomyces spp. against ESBL -and KPC- producing Enterobacteriaceae and MRSA.
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| Title: | Antagonistic activity of native Streptomyces spp. against ESBL -and KPC- producing Enterobacteriaceae and MRSA. |
|---|---|
| Alternate Title: | Actividad antagónica de Streptomyces spp. nativas frente a Enterobacteriaceae productoras de BLEE y KPC, y a MRSA. |
| Authors: | Osorio, Víctor Manuel1 victor.osorio@colmayor.edu.co, Mejía, Alejandro2, Correa, Elizabeth3, Ochoa, Ana María2, Rada, Ana Mercedes1, Martínez, José Gregorio1 |
| Source: | NOVA: Publicación Científica en Ciencias Biomédicas. jul-dic2025, Vol. 23 Issue 45, p7-25. 19p. |
| Subjects: | STREPTOMYCES, METHICILLIN-resistant staphylococcus aureus, CARBAPENEM-resistant bacteria, ANTI-infective agents, RHIZOBACTERIA, ORGANIC wastes, BETA lactamases, DRUG resistance in bacteria |
| Abstract (English): | Introduction. The search for microorganisms producing antimicrobial compounds represents a strategy to address the global crisis of antibiotic resistance. Identification of bacteria of the genus Streptomyces with antagonistic activity against resistant bacteria is the initial step for the subsequent recovery of effective antibacterial metabolites for the inhibition of these resistant organisms. Objectives. Recognize promising sources to isolate filamentous bacteria able to inhibit clinical isolates, including ESBL- and KPC- producing Enterobacteriaceae and MRSA. Methods. Actinobacteria were isolated from rhizospheres and a compost system. MRSA, Escherichia coli, and Klebsiella pneumoniae clinical isolates were chosen to address antagonism tests. Antibacterial activity was recorded by a cross-streak method. Streptomyces isolates were characterized according to International Streptomyces Project and the native isolates with antimicrobial activity were identified by molecular techniques. Results. Nine isolates of actinobacteria with activity against resistant-antibiotic bacteria were obtained, two from the avocado rhizosphere, four from a living fence and three from a composting system. Two of the isolates showed activity against all the tested antibiotic-resistant bacteria. Molecular taxonomic identification found S. jumonjinensis S. bacillaris, S. prasinus, S. microflavus, and S. cadmiisoli as putative species for native isolates. Conclusions. Streptomyces with antibacterial activities against ESBL and KPC-producing Enterobacteria and MRSA have been isolated and the potential of rhizospheres and compost systems for obtaining antibiotic-producing bacteria was validated. Native isolates exhibited common traits for Streptomyces; although NCBI's Blast did not show a resolution to identification, EzBioCloud 16S-based identification was able to accurately detect the identity of the isolates down to the species level. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Introducción. La búsqueda de microorganismos productores de compuestos antimicrobianos representa una estrategia para enfrentar la crisis global de la resistencia a los antibióticos. La identificación de bacterias del género Streptomyces con actividad antagónica contra bacterias multirresistentes es el paso inicial para recuperar metabolitos antibacterianos efectivos para inhibir estos organismos. Objetivos. Reconocer fuentes promisorias para aislar bacterias filamentosas capaces de inhibir aislamientos clínicos incluyendo Enterobacteriaceae productoras de BLEES y KPC, y MRSA. Métodos. Se aislaron actinobacterias de rizosferas y un sistema de compostaje. Se eligieron aislamientos clínicos de MRSA, Escherichia coli, and Klebsiella pneumoniae resistentes para realizar las pruebas de antagonismo. La actividad antibacteriana se registró mediante un método de estrías cruzadas. Los aislamientos de Streptomyces se caracterizaron según el Proyecto Internacional Streptomyces y se identificaron mediante técnicas moleculares. Resultados. Se obtuvieron nueve aislados de actinobacterias con actividad contra bacterias resistentes, dos de la rizosfera de aguacate, cuatro de un cerco vivo y tres de un sistema de compostaje; dos mostraron actividad contra todas las bacterias resistentes evaluadas. La identificación taxonómica molecular encontró S. jumonjinensis, S. bacillaris, S. prasinus, S. microflavus y S. cadmiisoli como especies putativas para los aislados nativos. Conclusiones. Se aislaron Streptomyces antagónicas contra Enterobacterias productoras de BLEE y KPC, y MRSA, validando el potencial de las rizosferas y los sistemas de compostaje para obtener bacterias productoras de antibióticos. Los aislados nativos presentaron características de Streptomyces; aunque el Blast de NCBI no mostró una resolución suficiente, EzBioCloud detectó la identidad de los aislados hasta especie. [ABSTRACT FROM AUTHOR] |
| Copyright of NOVA: Publicación Científica en Ciencias Biomédicas is the property of Universidad Colegio Mayor de Cundinamarca and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
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| Header | DbId: lth DbLabel: MedicLatina An: 192279497 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Antagonistic activity of native Streptomyces spp. against ESBL -and KPC- producing Enterobacteriaceae and MRSA. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: Actividad antagónica de Streptomyces spp. nativas frente a Enterobacteriaceae productoras de BLEE y KPC, y a MRSA. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Osorio%2C+Víctor+Manuel%22">Osorio, Víctor Manuel</searchLink><relatesTo>1</relatesTo><i> victor.osorio@colmayor.edu.co</i><br /><searchLink fieldCode="AR" term="%22Mejía%2C+Alejandro%22">Mejía, Alejandro</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Correa%2C+Elizabeth%22">Correa, Elizabeth</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Ochoa%2C+Ana+María%22">Ochoa, Ana María</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Rada%2C+Ana+Mercedes%22">Rada, Ana Mercedes</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Martínez%2C+José+Gregorio%22">Martínez, José Gregorio</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22NOVA%3A+Publicación+Científica+en+Ciencias+Biomédicas%22">NOVA: Publicación Científica en Ciencias Biomédicas</searchLink>. jul-dic2025, Vol. 23 Issue 45, p7-25. 19p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22STREPTOMYCES%22">STREPTOMYCES</searchLink><br /><searchLink fieldCode="DE" term="%22METHICILLIN-resistant+staphylococcus+aureus%22">METHICILLIN-resistant staphylococcus aureus</searchLink><br /><searchLink fieldCode="DE" term="%22CARBAPENEM-resistant+bacteria%22">CARBAPENEM-resistant bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22ANTI-infective+agents%22">ANTI-infective agents</searchLink><br /><searchLink fieldCode="DE" term="%22RHIZOBACTERIA%22">RHIZOBACTERIA</searchLink><br /><searchLink fieldCode="DE" term="%22ORGANIC+wastes%22">ORGANIC wastes</searchLink><br /><searchLink fieldCode="DE" term="%22BETA+lactamases%22">BETA lactamases</searchLink><br /><searchLink fieldCode="DE" term="%22DRUG+resistance+in+bacteria%22">DRUG resistance in bacteria</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: Introduction. The search for microorganisms producing antimicrobial compounds represents a strategy to address the global crisis of antibiotic resistance. Identification of bacteria of the genus Streptomyces with antagonistic activity against resistant bacteria is the initial step for the subsequent recovery of effective antibacterial metabolites for the inhibition of these resistant organisms. Objectives. Recognize promising sources to isolate filamentous bacteria able to inhibit clinical isolates, including ESBL- and KPC- producing Enterobacteriaceae and MRSA. Methods. Actinobacteria were isolated from rhizospheres and a compost system. MRSA, Escherichia coli, and Klebsiella pneumoniae clinical isolates were chosen to address antagonism tests. Antibacterial activity was recorded by a cross-streak method. Streptomyces isolates were characterized according to International Streptomyces Project and the native isolates with antimicrobial activity were identified by molecular techniques. Results. Nine isolates of actinobacteria with activity against resistant-antibiotic bacteria were obtained, two from the avocado rhizosphere, four from a living fence and three from a composting system. Two of the isolates showed activity against all the tested antibiotic-resistant bacteria. Molecular taxonomic identification found S. jumonjinensis S. bacillaris, S. prasinus, S. microflavus, and S. cadmiisoli as putative species for native isolates. Conclusions. Streptomyces with antibacterial activities against ESBL and KPC-producing Enterobacteria and MRSA have been isolated and the potential of rhizospheres and compost systems for obtaining antibiotic-producing bacteria was validated. Native isolates exhibited common traits for Streptomyces; although NCBI's Blast did not show a resolution to identification, EzBioCloud 16S-based identification was able to accurately detect the identity of the isolates down to the species level. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Introducción. La búsqueda de microorganismos productores de compuestos antimicrobianos representa una estrategia para enfrentar la crisis global de la resistencia a los antibióticos. La identificación de bacterias del género Streptomyces con actividad antagónica contra bacterias multirresistentes es el paso inicial para recuperar metabolitos antibacterianos efectivos para inhibir estos organismos. Objetivos. Reconocer fuentes promisorias para aislar bacterias filamentosas capaces de inhibir aislamientos clínicos incluyendo Enterobacteriaceae productoras de BLEES y KPC, y MRSA. Métodos. Se aislaron actinobacterias de rizosferas y un sistema de compostaje. Se eligieron aislamientos clínicos de MRSA, Escherichia coli, and Klebsiella pneumoniae resistentes para realizar las pruebas de antagonismo. La actividad antibacteriana se registró mediante un método de estrías cruzadas. Los aislamientos de Streptomyces se caracterizaron según el Proyecto Internacional Streptomyces y se identificaron mediante técnicas moleculares. Resultados. Se obtuvieron nueve aislados de actinobacterias con actividad contra bacterias resistentes, dos de la rizosfera de aguacate, cuatro de un cerco vivo y tres de un sistema de compostaje; dos mostraron actividad contra todas las bacterias resistentes evaluadas. La identificación taxonómica molecular encontró S. jumonjinensis, S. bacillaris, S. prasinus, S. microflavus y S. cadmiisoli como especies putativas para los aislados nativos. Conclusiones. Se aislaron Streptomyces antagónicas contra Enterobacterias productoras de BLEE y KPC, y MRSA, validando el potencial de las rizosferas y los sistemas de compostaje para obtener bacterias productoras de antibióticos. Los aislados nativos presentaron características de Streptomyces; aunque el Blast de NCBI no mostró una resolución suficiente, EzBioCloud detectó la identidad de los aislados hasta especie. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of NOVA: Publicación Científica en Ciencias Biomédicas is the property of Universidad Colegio Mayor de Cundinamarca and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 19 StartPage: 7 Subjects: – SubjectFull: STREPTOMYCES Type: general – SubjectFull: METHICILLIN-resistant staphylococcus aureus Type: general – SubjectFull: CARBAPENEM-resistant bacteria Type: general – SubjectFull: ANTI-infective agents Type: general – SubjectFull: RHIZOBACTERIA Type: general – SubjectFull: ORGANIC wastes Type: general – SubjectFull: BETA lactamases Type: general – SubjectFull: DRUG resistance in bacteria Type: general Titles: – TitleFull: Antagonistic activity of native Streptomyces spp. against ESBL -and KPC- producing Enterobacteriaceae and MRSA. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Osorio, Víctor Manuel – PersonEntity: Name: NameFull: Mejía, Alejandro – PersonEntity: Name: NameFull: Correa, Elizabeth – PersonEntity: Name: NameFull: Ochoa, Ana María – PersonEntity: Name: NameFull: Rada, Ana Mercedes – PersonEntity: Name: NameFull: Martínez, José Gregorio IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: jul-dic2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 17942470 Numbering: – Type: volume Value: 23 – Type: issue Value: 45 Titles: – TitleFull: NOVA: Publicación Científica en Ciencias Biomédicas Type: main |
| ResultId | 1 |
