Evaluación de la inmunidad celular específica frente al SARS-CoV-2: técnicas para su medición.
Saved in:
| Title: | Evaluación de la inmunidad celular específica frente al SARS-CoV-2: técnicas para su medición. |
|---|---|
| Alternate Title: | Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement. |
| Authors: | Enciso-Benavides, Javier1 javier.enciso.benavides@gmail.com, Garcia-Paita, Marlon1 marlon.garcia@unmsm.edu.pe, Alvarez-Vega, Santiago1 santiagoav1913@gmail.com, Tapia-Rojas, Salyoc1 stapiar@cientifica.edu.pe, Mayanga-Herrera, Ana1 amayanga@cientifica.edu.pe, Pons, Maria J.2 ma.pons.cas@gmail.com |
| Source: | Revista del Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo. Oct-Dec2025, Vol. 18 Issue 4, p1-12. 12p. |
| Subjects: | Cellular immunity, T cells, Immunoassay, Flow cytometry, Vaccination, Cytokines, Molecular diagnosis, SARS-CoV-2 |
| Abstract (English): | Understanding the immune response to infection or vaccination against SARS-CoV-2 has become a key objective for the understanding of the disease, its progression, and protection against future infections or possible new variants that may emerge of the virus. The aim of the present review is to focus on the relevance of the cellular immune response and to summarize the different methodologies described for the study of this memory Tcell-related response, either after exposure to the virus or vaccination against SARS-CoV2. For this purpose, cell proliferation assays (CFSE, 3H-thymidine), cell typing methods (flow cytometry), immunological assays based on the measurement of cytokines (ELISA, ELISPOT, suspension multiplex) and analysis of genes related to cellular immunity by molecular techniques (RT-qPCR or qPCR and sequencing) have been included [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Comprender la respuesta inmunitaria frente a la infección o vacunación contra SARSCoV-2 se ha convertido en un objetivo clave para entender la enfermedad, su progresión, y protección frente a futuras infecciones o posibles nuevas variantes que puedan surgir del virus. El objetivo de la presente revisión es destacar la relevancia de la respuesta inmunológica celular y resumir las diferentes metodologías descritas para el estudio de esta respuesta mediada por células T, de memoria, tanto tras la exposición al virus como de la vacunación frente al SARS-CoV-2. Para ello, se incluyen los ensayos de proliferación celular (CFSE, 3H- timidina), métodos de tipificación celular (citometría de flujo), los ensayos inmunológicos basados en la medición de citocinas (ELISA, ELISPOT, Multiplexado en suspensión) y el análisis de genes relacionados con la inmunidad celular mediante técnicas moleculares (RT-qPCR o qPCR y secuenciación). [ABSTRACT FROM AUTHOR] |
| Copyright of Revista del Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo is the property of Cuerpo Medico del Hospital Nacional Almanzor Aguinaga Asenjo and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
|---|---|
| Header | DbId: lth DbLabel: MedicLatina An: 195006021 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Evaluación de la inmunidad celular específica frente al SARS-CoV-2: técnicas para su medición. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Enciso-Benavides%2C+Javier%22">Enciso-Benavides, Javier</searchLink><relatesTo>1</relatesTo><i> javier.enciso.benavides@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Garcia-Paita%2C+Marlon%22">Garcia-Paita, Marlon</searchLink><relatesTo>1</relatesTo><i> marlon.garcia@unmsm.edu.pe</i><br /><searchLink fieldCode="AR" term="%22Alvarez-Vega%2C+Santiago%22">Alvarez-Vega, Santiago</searchLink><relatesTo>1</relatesTo><i> santiagoav1913@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Tapia-Rojas%2C+Salyoc%22">Tapia-Rojas, Salyoc</searchLink><relatesTo>1</relatesTo><i> stapiar@cientifica.edu.pe</i><br /><searchLink fieldCode="AR" term="%22Mayanga-Herrera%2C+Ana%22">Mayanga-Herrera, Ana</searchLink><relatesTo>1</relatesTo><i> amayanga@cientifica.edu.pe</i><br /><searchLink fieldCode="AR" term="%22Pons%2C+Maria+J%2E%22">Pons, Maria J.</searchLink><relatesTo>2</relatesTo><i> ma.pons.cas@gmail.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Revista+del+Cuerpo+Médico+del+Hospital+Nacional+Almanzor+Aguinaga+Asenjo%22">Revista del Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo</searchLink>. Oct-Dec2025, Vol. 18 Issue 4, p1-12. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Cellular+immunity%22">Cellular immunity</searchLink><br /><searchLink fieldCode="DE" term="%22T+cells%22">T cells</searchLink><br /><searchLink fieldCode="DE" term="%22Immunoassay%22">Immunoassay</searchLink><br /><searchLink fieldCode="DE" term="%22Flow+cytometry%22">Flow cytometry</searchLink><br /><searchLink fieldCode="DE" term="%22Vaccination%22">Vaccination</searchLink><br /><searchLink fieldCode="DE" term="%22Cytokines%22">Cytokines</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+diagnosis%22">Molecular diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22SARS-CoV-2%22">SARS-CoV-2</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: Understanding the immune response to infection or vaccination against SARS-CoV-2 has become a key objective for the understanding of the disease, its progression, and protection against future infections or possible new variants that may emerge of the virus. The aim of the present review is to focus on the relevance of the cellular immune response and to summarize the different methodologies described for the study of this memory Tcell-related response, either after exposure to the virus or vaccination against SARS-CoV2. For this purpose, cell proliferation assays (CFSE, 3H-thymidine), cell typing methods (flow cytometry), immunological assays based on the measurement of cytokines (ELISA, ELISPOT, suspension multiplex) and analysis of genes related to cellular immunity by molecular techniques (RT-qPCR or qPCR and sequencing) have been included [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Comprender la respuesta inmunitaria frente a la infección o vacunación contra SARSCoV-2 se ha convertido en un objetivo clave para entender la enfermedad, su progresión, y protección frente a futuras infecciones o posibles nuevas variantes que puedan surgir del virus. El objetivo de la presente revisión es destacar la relevancia de la respuesta inmunológica celular y resumir las diferentes metodologías descritas para el estudio de esta respuesta mediada por células T, de memoria, tanto tras la exposición al virus como de la vacunación frente al SARS-CoV-2. Para ello, se incluyen los ensayos de proliferación celular (CFSE, 3H- timidina), métodos de tipificación celular (citometría de flujo), los ensayos inmunológicos basados en la medición de citocinas (ELISA, ELISPOT, Multiplexado en suspensión) y el análisis de genes relacionados con la inmunidad celular mediante técnicas moleculares (RT-qPCR o qPCR y secuenciación). [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Revista del Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo is the property of Cuerpo Medico del Hospital Nacional Almanzor Aguinaga Asenjo and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lth&AN=195006021 |
| RecordInfo | BibRecord: BibEntity: Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 12 StartPage: 1 Subjects: – SubjectFull: Cellular immunity Type: general – SubjectFull: T cells Type: general – SubjectFull: Immunoassay Type: general – SubjectFull: Flow cytometry Type: general – SubjectFull: Vaccination Type: general – SubjectFull: Cytokines Type: general – SubjectFull: Molecular diagnosis Type: general – SubjectFull: SARS-CoV-2 Type: general Titles: – TitleFull: Evaluación de la inmunidad celular específica frente al SARS-CoV-2: técnicas para su medición. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Enciso-Benavides, Javier – PersonEntity: Name: NameFull: Garcia-Paita, Marlon – PersonEntity: Name: NameFull: Alvarez-Vega, Santiago – PersonEntity: Name: NameFull: Tapia-Rojas, Salyoc – PersonEntity: Name: NameFull: Mayanga-Herrera, Ana – PersonEntity: Name: NameFull: Pons, Maria J. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: Oct-Dec2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 22255109 Numbering: – Type: volume Value: 18 – Type: issue Value: 4 Titles: – TitleFull: Revista del Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo Type: main |
| ResultId | 1 |
