View in EDS PDF Full Text

PREPARATION AND EVALUATION OF VI POLYSACCHARIDE-PROTEIN CONJUGATES COMPOSED OF TETANUS TOXOID OR OMP FROM N. MENINGITIDIS SEROGRUPO B AS A CARRIER.

Saved in:
Bibliographic Details
Title: PREPARATION AND EVALUATION OF VI POLYSACCHARIDE-PROTEIN CONJUGATES COMPOSED OF TETANUS TOXOID OR OMP FROM N. MENINGITIDIS SEROGRUPO B AS A CARRIER.
Authors: Cuello, M.1 mcuello@finlay.edu.cu, Cabrera, O.1, Soto, C. R.1, Pérez, O.1, Del Campo, J.1, Taboada, C. M.1, Fariñas, Mildrey1, Sierra, Gustavo1
Source: Revista VacciMonitor (Vacunología y Temas Afines). Oct2002, Vol. 11 Issue 4, p1. 1p.
Subjects: POLYSACCHARIDES, ANTIGENS, CLINICAL trials, TYPHOID fever, IMMUNOGLOBULINS, IMMUNE response, NEISSERIA meningitidis, TETANUS
Abstract: The polysaccharide Vi has proven to be a protective antigen in young adults. In controlled clinical trials in areas with high rates of typhoid fever, the protective efficacy of the Vi was approximately 70%. However, methods were used, to synthesize polysaccharide Vi-protein conjugates in order to both enhance the antibody response and confer T-dependent properties to the polysaccharide in populations at high risk. In this study the immunogenicity and type of immune response (Th2, IgG1 or Th1, IgG2a) to a polysaccharide Vi-protein conjugates was evaluated in mice. The purified polysaccharide was linked to carrier proteins via carbodiímida-mediated reaction, with adipic acid dihydrazide as spacer arms. This synthetic scheme was reproducible, provided high yields of Vi-protein conjugates, and was applicable to several medically relevant proteins such as Outer Membrane Vesicle from Neisseria meningitidis serogrupo B and tetanus toxoids. The resultant conjugate induced mainly IgG2a subclass anti-Vi and was more immunogenic than the Vi alone in mice. In contrast to the T-independent properties of the Vi, conjugates of this polysaccharide with several medically relevant proteins induced booster responses and induce an increase the duration of their immune response in the time, in mice. The serum of animals inoculated with conjugate was shown high titers IgG anti-carrier proteins, like proteins native. [ABSTRACT FROM AUTHOR]
Copyright of Revista VacciMonitor (Vacunología y Temas Afines) is the property of Vacunas Finlay S.A. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: MedicLatina
FullText Links:
  – Type: pdflink
Text:
  Availability: 0
Header DbId: lth
DbLabel: MedicLatina
An: 27105105
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: PREPARATION AND EVALUATION OF VI POLYSACCHARIDE-PROTEIN CONJUGATES COMPOSED OF TETANUS TOXOID OR OMP FROM N. MENINGITIDIS SEROGRUPO B AS A CARRIER.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Cuello%2C+M%2E%22">Cuello, M.</searchLink><relatesTo>1</relatesTo><i> mcuello@finlay.edu.cu</i><br /><searchLink fieldCode="AR" term="%22Cabrera%2C+O%2E%22">Cabrera, O.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Soto%2C+C%2E+R%2E%22">Soto, C. R.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Pérez%2C+O%2E%22">Pérez, O.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Del+Campo%2C+J%2E%22">Del Campo, J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Taboada%2C+C%2E+M%2E%22">Taboada, C. M.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Fariñas%2C+Mildrey%22">Fariñas, Mildrey</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Sierra%2C+Gustavo%22">Sierra, Gustavo</searchLink><relatesTo>1</relatesTo>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Revista+VacciMonitor+%28Vacunología+y+Temas+Afines%29%22">Revista VacciMonitor (Vacunología y Temas Afines)</searchLink>. Oct2002, Vol. 11 Issue 4, p1. 1p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22POLYSACCHARIDES%22">POLYSACCHARIDES</searchLink><br /><searchLink fieldCode="DE" term="%22ANTIGENS%22">ANTIGENS</searchLink><br /><searchLink fieldCode="DE" term="%22CLINICAL+trials%22">CLINICAL trials</searchLink><br /><searchLink fieldCode="DE" term="%22TYPHOID+fever%22">TYPHOID fever</searchLink><br /><searchLink fieldCode="DE" term="%22IMMUNOGLOBULINS%22">IMMUNOGLOBULINS</searchLink><br /><searchLink fieldCode="DE" term="%22IMMUNE+response%22">IMMUNE response</searchLink><br /><searchLink fieldCode="DE" term="%22NEISSERIA+meningitidis%22">NEISSERIA meningitidis</searchLink><br /><searchLink fieldCode="DE" term="%22TETANUS%22">TETANUS</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The polysaccharide Vi has proven to be a protective antigen in young adults. In controlled clinical trials in areas with high rates of typhoid fever, the protective efficacy of the Vi was approximately 70%. However, methods were used, to synthesize polysaccharide Vi-protein conjugates in order to both enhance the antibody response and confer T-dependent properties to the polysaccharide in populations at high risk. In this study the immunogenicity and type of immune response (Th2, IgG1 or Th1, IgG2a) to a polysaccharide Vi-protein conjugates was evaluated in mice. The purified polysaccharide was linked to carrier proteins via carbodiímida-mediated reaction, with adipic acid dihydrazide as spacer arms. This synthetic scheme was reproducible, provided high yields of Vi-protein conjugates, and was applicable to several medically relevant proteins such as Outer Membrane Vesicle from Neisseria meningitidis serogrupo B and tetanus toxoids. The resultant conjugate induced mainly IgG2a subclass anti-Vi and was more immunogenic than the Vi alone in mice. In contrast to the T-independent properties of the Vi, conjugates of this polysaccharide with several medically relevant proteins induced booster responses and induce an increase the duration of their immune response in the time, in mice. The serum of animals inoculated with conjugate was shown high titers IgG anti-carrier proteins, like proteins native. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Revista VacciMonitor (Vacunología y Temas Afines) is the property of Vacunas Finlay S.A. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lth&AN=27105105
RecordInfo BibRecord:
  BibEntity:
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: 1
    Subjects:
      – SubjectFull: POLYSACCHARIDES
        Type: general
      – SubjectFull: ANTIGENS
        Type: general
      – SubjectFull: CLINICAL trials
        Type: general
      – SubjectFull: TYPHOID fever
        Type: general
      – SubjectFull: IMMUNOGLOBULINS
        Type: general
      – SubjectFull: IMMUNE response
        Type: general
      – SubjectFull: NEISSERIA meningitidis
        Type: general
      – SubjectFull: TETANUS
        Type: general
    Titles:
      – TitleFull: PREPARATION AND EVALUATION OF VI POLYSACCHARIDE-PROTEIN CONJUGATES COMPOSED OF TETANUS TOXOID OR OMP FROM N. MENINGITIDIS SEROGRUPO B AS A CARRIER.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Cuello, M.
      – PersonEntity:
          Name:
            NameFull: Cabrera, O.
      – PersonEntity:
          Name:
            NameFull: Soto, C. R.
      – PersonEntity:
          Name:
            NameFull: Pérez, O.
      – PersonEntity:
          Name:
            NameFull: Del Campo, J.
      – PersonEntity:
          Name:
            NameFull: Taboada, C. M.
      – PersonEntity:
          Name:
            NameFull: Fariñas, Mildrey
      – PersonEntity:
          Name:
            NameFull: Sierra, Gustavo
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 10
              Text: Oct2002
              Type: published
              Y: 2002
          Identifiers:
            – Type: issn-print
              Value: 1025028X
          Numbering:
            – Type: volume
              Value: 11
            – Type: issue
              Value: 4
          Titles:
            – TitleFull: Revista VacciMonitor (Vacunología y Temas Afines)
              Type: main
ResultId 1