Detección de Bacillus cereus toxigénicos en productos lácteos con especias y leches deshidratadas colectadas en Costa Rica.
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| Title: | Detección de Bacillus cereus toxigénicos en productos lácteos con especias y leches deshidratadas colectadas en Costa Rica. |
|---|---|
| Alternate Title: | Toxigenic Bacillus cereus detection in lactic products with spices and dehydrated milk collected in Costa Rica. |
| Authors: | Blanco, Walter, Arias, María Laura, Pérez, Cristian, Rodríguez, César, Chaves, Carolina |
| Source: | Archivos Latinoamericanos de Nutrición. dic2009, Vol. 59 Issue 4, p402-406. 5p. 3 Charts. |
| Subjects: | BACILLUS (Bacteria), GRAM-positive bacteria, FOODBORNE diseases, DIARRHEA |
| Geographic Terms: | SAN Jose (Costa Rica), COSTA Rica |
| Abstract (English): | Bacillus cereus is a Gram positive rod widely distributed in nature and associated to different types of food that, under some circumstances, may cause pathology to human beings. Diarrheic and emetic strains have been described based on the type of toxins produced. In order to determine the risk to health represented by this bacteria, the toxigenic potential of strains isolated from cheese with spices, spread cheese with spices and dehydrated milk, all sold in San José, Costa Rica, were determined using a multiplex PCR technique with oligonucleotides specific for the genes coding toxins HBL and Nhe. From 45 samples collected, 15 isolates of B cereus were obtained (60% coming from spread cheese with spices 7% from dehydrated milk and 13% from cheese with spices). All the strains analyzed presented at least one of the genes analyzed; six of them, coming from dehydrated milk and spread cheese, showed molecular evidence of the genes nheB, nheA, nheC, hblD, hblA y hblC, confirming the correlation described for the presence of operons codifying for HBL and Nhe. Nevertheless, the no detection of a gene cannot be considered as a definitive proof of its absence, given the existence of polymorphism in the sequences of the genes analyzed. The results obtained show that multiple of the B cereus strains found in lactic products from Costa Rica have the necessary genes for synthesizing toxins, so the correct handling of these products is very important since they can represent a risk for public health. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Bacillus cereus es un bacilo Gram-positivo de amplia distribución en la naturaleza y asociado a diversos tipos de alimentos que, bajo ciertas circunstancias causa patologías de diversa índole. Se han descrito cepas diarrogénicas y eméticas basándose en el tipo de toxinas producidas. Con el fin de determinar el riesgo de salud que representa en esta bacteria, se determinó el potencial toxigénico de cepas aisladas a partir de quesos maduros con especias, quesos crema con especias y leches deshidratadas expendidas en San José, Costa Rica, mediante un PCR múltiple con oligonucleótidos específicos para los genes codificantes de las toxinas HBL y Nhe. A partir de 45 muestras recolectadas, se obtuvieron 15 aislamientos de B. cereus, (60% provenientes de queso crema con especias, 7% de leche deshidratada y 13% de queso maduro con especias). Todas las cepas estudiadas presentaron al menos uno de los genes analizados, seis de ellas provenientes de leches deshidratadas y quesos crema, exhibieron evidencia molecular de los genes nheB, nheA, nheC, hblD, hblA y hblC lo cual permite confirmar la correlación descrita para la presencia de los operones codificantes para la HBL y la Nhe. No obstante, la no detección de un gen no puede considerarse como prueba definitiva de su ausencia dado que existen polimorfismos en las secuencias de los genes aquí analizados. Los resultados demuestran que múltiples cepas de B. cereus encontradas en lácteos comprados en Costa Rica contienen los genes necesarios para sintetizar toxinas, por lo tanto es importante el manejo adecuado de estos productos ya que eventualmente pueden representar un riesgo para la salud pública. [ABSTRACT FROM AUTHOR] |
| Copyright of Archivos Latinoamericanos de Nutrición is the property of Sociedad Latinoamericana de Nutricion and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: lth DbLabel: MedicLatina An: 48636056 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Detección de Bacillus cereus toxigénicos en productos lácteos con especias y leches deshidratadas colectadas en Costa Rica. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: Toxigenic Bacillus cereus detection in lactic products with spices and dehydrated milk collected in Costa Rica. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Blanco%2C+Walter%22">Blanco, Walter</searchLink><br /><searchLink fieldCode="AR" term="%22Arias%2C+María+Laura%22">Arias, María Laura</searchLink><br /><searchLink fieldCode="AR" term="%22Pérez%2C+Cristian%22">Pérez, Cristian</searchLink><br /><searchLink fieldCode="AR" term="%22Rodríguez%2C+César%22">Rodríguez, César</searchLink><br /><searchLink fieldCode="AR" term="%22Chaves%2C+Carolina%22">Chaves, Carolina</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Archivos+Latinoamericanos+de+Nutrición%22">Archivos Latinoamericanos de Nutrición</searchLink>. dic2009, Vol. 59 Issue 4, p402-406. 5p. 3 Charts. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22BACILLUS+%28Bacteria%29%22">BACILLUS (Bacteria)</searchLink><br /><searchLink fieldCode="DE" term="%22GRAM-positive+bacteria%22">GRAM-positive bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22FOODBORNE+diseases%22">FOODBORNE diseases</searchLink><br /><searchLink fieldCode="DE" term="%22DIARRHEA%22">DIARRHEA</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22SAN+Jose+%28Costa+Rica%29%22">SAN Jose (Costa Rica)</searchLink><br /><searchLink fieldCode="DE" term="%22COSTA+Rica%22">COSTA Rica</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: Bacillus cereus is a Gram positive rod widely distributed in nature and associated to different types of food that, under some circumstances, may cause pathology to human beings. Diarrheic and emetic strains have been described based on the type of toxins produced. In order to determine the risk to health represented by this bacteria, the toxigenic potential of strains isolated from cheese with spices, spread cheese with spices and dehydrated milk, all sold in San José, Costa Rica, were determined using a multiplex PCR technique with oligonucleotides specific for the genes coding toxins HBL and Nhe. From 45 samples collected, 15 isolates of B cereus were obtained (60% coming from spread cheese with spices 7% from dehydrated milk and 13% from cheese with spices). All the strains analyzed presented at least one of the genes analyzed; six of them, coming from dehydrated milk and spread cheese, showed molecular evidence of the genes nheB, nheA, nheC, hblD, hblA y hblC, confirming the correlation described for the presence of operons codifying for HBL and Nhe. Nevertheless, the no detection of a gene cannot be considered as a definitive proof of its absence, given the existence of polymorphism in the sequences of the genes analyzed. The results obtained show that multiple of the B cereus strains found in lactic products from Costa Rica have the necessary genes for synthesizing toxins, so the correct handling of these products is very important since they can represent a risk for public health. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Bacillus cereus es un bacilo Gram-positivo de amplia distribución en la naturaleza y asociado a diversos tipos de alimentos que, bajo ciertas circunstancias causa patologías de diversa índole. Se han descrito cepas diarrogénicas y eméticas basándose en el tipo de toxinas producidas. Con el fin de determinar el riesgo de salud que representa en esta bacteria, se determinó el potencial toxigénico de cepas aisladas a partir de quesos maduros con especias, quesos crema con especias y leches deshidratadas expendidas en San José, Costa Rica, mediante un PCR múltiple con oligonucleótidos específicos para los genes codificantes de las toxinas HBL y Nhe. A partir de 45 muestras recolectadas, se obtuvieron 15 aislamientos de B. cereus, (60% provenientes de queso crema con especias, 7% de leche deshidratada y 13% de queso maduro con especias). Todas las cepas estudiadas presentaron al menos uno de los genes analizados, seis de ellas provenientes de leches deshidratadas y quesos crema, exhibieron evidencia molecular de los genes nheB, nheA, nheC, hblD, hblA y hblC lo cual permite confirmar la correlación descrita para la presencia de los operones codificantes para la HBL y la Nhe. No obstante, la no detección de un gen no puede considerarse como prueba definitiva de su ausencia dado que existen polimorfismos en las secuencias de los genes aquí analizados. Los resultados demuestran que múltiples cepas de B. cereus encontradas en lácteos comprados en Costa Rica contienen los genes necesarios para sintetizar toxinas, por lo tanto es importante el manejo adecuado de estos productos ya que eventualmente pueden representar un riesgo para la salud pública. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Archivos Latinoamericanos de Nutrición is the property of Sociedad Latinoamericana de Nutricion and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 5 StartPage: 402 Subjects: – SubjectFull: BACILLUS (Bacteria) Type: general – SubjectFull: GRAM-positive bacteria Type: general – SubjectFull: FOODBORNE diseases Type: general – SubjectFull: DIARRHEA Type: general – SubjectFull: SAN Jose (Costa Rica) Type: general – SubjectFull: COSTA Rica Type: general Titles: – TitleFull: Detección de Bacillus cereus toxigénicos en productos lácteos con especias y leches deshidratadas colectadas en Costa Rica. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Blanco, Walter – PersonEntity: Name: NameFull: Arias, María Laura – PersonEntity: Name: NameFull: Pérez, Cristian – PersonEntity: Name: NameFull: Rodríguez, César – PersonEntity: Name: NameFull: Chaves, Carolina IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: dic2009 Type: published Y: 2009 Identifiers: – Type: issn-print Value: 00040622 Numbering: – Type: volume Value: 59 – Type: issue Value: 4 Titles: – TitleFull: Archivos Latinoamericanos de Nutrición Type: main |
| ResultId | 1 |
