Análisis comparativo de seis genomas del complejo Mycobacterium tuberculosis.
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| Title: | Análisis comparativo de seis genomas del complejo Mycobacterium tuberculosis. |
|---|---|
| Alternate Title: | Comparative analysis of six Mycobacterium tuberculosis complex genomes. |
| Authors: | Chaves, Diego1, Sandoval, Andrea2, Rodríguez, Luis1, García, Juan C.2, Restrepo, Silvia1, Zambrano, María Mercedes2 mzambrano@corpogen.org |
| Source: | Biomédica: Revista del Instituto Nacional de Salud. mar2010, Vol. 30 Issue 1, p23-31. 9p. 1 Diagram, 3 Charts, 1 Graph. |
| Subjects: | MYCOBACTERIUM tuberculosis, GENOMES, TRANSPOSONS, BACTERIOPHAGES, PHYSIOLOGIC strain, COMPARATIVE studies |
| Abstract (English): | Introduction. A growing number of sequenced genomes belonging to the Mycobacterium tuberculosis complex has enabled a comparison of strain traits and genomic constitution. These analyses may reveal mechanisms of evolution and genomic variation relevant to tuberculosis pathogenesis. Objective. Multiple alignments were used to analyze the differences between six genomes of the M. tuberculosis complex and to locate regions of variation that may lead to improvements in species identification or in their treatment. Materials and methods. The Mauve software package was used to perform a multiple alignment of 6 genomes belonging to the M. tuberculosis complex. Regions exclusive to each genome were annotated using the TB database. Results. Percent similarity among the six genomes ranged between 96.1% and 97.8%. The annotation identified intergenic regions, regions associated with transposable elements of the PE-PGRS and PPE families, and regions associated with resistance against bacteriophage. Conclusions. In spite of the high genetic similarity among the tuberculosis strains, genomic variations were elucidated that may be relevant to differences in behavior and virulence, as well as for improvement of strain diagnosis. Regions encoding membrane-associated proteins, possibly related with antigenic variation and immune response, are particularly interesting for studies aimed at seeking tuberculosis treatments. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Introducción. El creciente número de genomas secuenciados pertenecientes al complejo Mycobacterium tuberculosis hace posible la comparación y el análisis genómico, que puede revelar importantes mecanismos de evolución y variación para entender la patogénesis de esta especie. Objetivo. Mediante el uso de alineamientos múltiples se pretendió analizar las diferencias entre seis genomas del complejo M. tuberculosis, para encontrar regiones de variación que conduzcan a mejoras en la identificación de estas especies o en el tratamiento. Materiales y métodos. Mediante el programa bioinformático Mauve, se realizaron alineamientos múltiples de seis genomas pertenecientes a especies del complejo M. tuberculosis. Las regiones genómicas exclusivas para cada genoma se anotaron usando la base de datos Tuberculosis Database. Resultados. El porcentaje de similitud entre los seis genomas analizados estuvo entre 96,1% y 97,8%. La anotación de las regiones exclusivas reveló la presencia de elementos de transposición, familias de proteínas PPE y PE-PGRS, regiones asociadas a resistencia contra bacteriófagos y regiones intergénicas. Conclusiones. A pesar de la gran similitud entre las cepas analizadas, existen variaciones entre ellas que pueden ser importantes para entender diferencias en comportamiento y virulencia, así como para mejorar los diagnósticos de cepas específicas. Regiones como aquéllas con genes para proteínas de membrana, posiblemente, relacionadas con la variación y la respuesta antigénica, son de particular interés para estudios futuros orientados a buscar tratamientos nuevos para el control de esta enfermedad. [ABSTRACT FROM AUTHOR] |
| Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
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| Items | – Name: Title Label: Title Group: Ti Data: Análisis comparativo de seis genomas del complejo Mycobacterium tuberculosis. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: Comparative analysis of six Mycobacterium tuberculosis complex genomes. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Chaves%2C+Diego%22">Chaves, Diego</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Sandoval%2C+Andrea%22">Sandoval, Andrea</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Rodríguez%2C+Luis%22">Rodríguez, Luis</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22García%2C+Juan+C%2E%22">García, Juan C.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Restrepo%2C+Silvia%22">Restrepo, Silvia</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zambrano%2C+María+Mercedes%22">Zambrano, María Mercedes</searchLink><relatesTo>2</relatesTo><i> mzambrano@corpogen.org</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biomédica%3A+Revista+del+Instituto+Nacional+de+Salud%22">Biomédica: Revista del Instituto Nacional de Salud</searchLink>. mar2010, Vol. 30 Issue 1, p23-31. 9p. 1 Diagram, 3 Charts, 1 Graph. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22MYCOBACTERIUM+tuberculosis%22">MYCOBACTERIUM tuberculosis</searchLink><br /><searchLink fieldCode="DE" term="%22GENOMES%22">GENOMES</searchLink><br /><searchLink fieldCode="DE" term="%22TRANSPOSONS%22">TRANSPOSONS</searchLink><br /><searchLink fieldCode="DE" term="%22BACTERIOPHAGES%22">BACTERIOPHAGES</searchLink><br /><searchLink fieldCode="DE" term="%22PHYSIOLOGIC+strain%22">PHYSIOLOGIC strain</searchLink><br /><searchLink fieldCode="DE" term="%22COMPARATIVE+studies%22">COMPARATIVE studies</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: Introduction. A growing number of sequenced genomes belonging to the Mycobacterium tuberculosis complex has enabled a comparison of strain traits and genomic constitution. These analyses may reveal mechanisms of evolution and genomic variation relevant to tuberculosis pathogenesis. Objective. Multiple alignments were used to analyze the differences between six genomes of the M. tuberculosis complex and to locate regions of variation that may lead to improvements in species identification or in their treatment. Materials and methods. The Mauve software package was used to perform a multiple alignment of 6 genomes belonging to the M. tuberculosis complex. Regions exclusive to each genome were annotated using the TB database. Results. Percent similarity among the six genomes ranged between 96.1% and 97.8%. The annotation identified intergenic regions, regions associated with transposable elements of the PE-PGRS and PPE families, and regions associated with resistance against bacteriophage. Conclusions. In spite of the high genetic similarity among the tuberculosis strains, genomic variations were elucidated that may be relevant to differences in behavior and virulence, as well as for improvement of strain diagnosis. Regions encoding membrane-associated proteins, possibly related with antigenic variation and immune response, are particularly interesting for studies aimed at seeking tuberculosis treatments. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Introducción. El creciente número de genomas secuenciados pertenecientes al complejo Mycobacterium tuberculosis hace posible la comparación y el análisis genómico, que puede revelar importantes mecanismos de evolución y variación para entender la patogénesis de esta especie. Objetivo. Mediante el uso de alineamientos múltiples se pretendió analizar las diferencias entre seis genomas del complejo M. tuberculosis, para encontrar regiones de variación que conduzcan a mejoras en la identificación de estas especies o en el tratamiento. Materiales y métodos. Mediante el programa bioinformático Mauve, se realizaron alineamientos múltiples de seis genomas pertenecientes a especies del complejo M. tuberculosis. Las regiones genómicas exclusivas para cada genoma se anotaron usando la base de datos Tuberculosis Database. Resultados. El porcentaje de similitud entre los seis genomas analizados estuvo entre 96,1% y 97,8%. La anotación de las regiones exclusivas reveló la presencia de elementos de transposición, familias de proteínas PPE y PE-PGRS, regiones asociadas a resistencia contra bacteriófagos y regiones intergénicas. Conclusiones. A pesar de la gran similitud entre las cepas analizadas, existen variaciones entre ellas que pueden ser importantes para entender diferencias en comportamiento y virulencia, así como para mejorar los diagnósticos de cepas específicas. Regiones como aquéllas con genes para proteínas de membrana, posiblemente, relacionadas con la variación y la respuesta antigénica, son de particular interés para estudios futuros orientados a buscar tratamientos nuevos para el control de esta enfermedad. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.7705/biomedica.v30i1.149 Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 9 StartPage: 23 Subjects: – SubjectFull: MYCOBACTERIUM tuberculosis Type: general – SubjectFull: GENOMES Type: general – SubjectFull: TRANSPOSONS Type: general – SubjectFull: BACTERIOPHAGES Type: general – SubjectFull: PHYSIOLOGIC strain Type: general – SubjectFull: COMPARATIVE studies Type: general Titles: – TitleFull: Análisis comparativo de seis genomas del complejo Mycobacterium tuberculosis. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Chaves, Diego – PersonEntity: Name: NameFull: Sandoval, Andrea – PersonEntity: Name: NameFull: Rodríguez, Luis – PersonEntity: Name: NameFull: García, Juan C. – PersonEntity: Name: NameFull: Restrepo, Silvia – PersonEntity: Name: NameFull: Zambrano, María Mercedes IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: mar2010 Type: published Y: 2010 Identifiers: – Type: issn-print Value: 01204157 Numbering: – Type: volume Value: 30 – Type: issue Value: 1 Titles: – TitleFull: Biomédica: Revista del Instituto Nacional de Salud Type: main |
| ResultId | 1 |
