Estudio comparativo de Wider, E-test y microdilución para la determinación de la sensibilidad a daptomicina y otros tres antimicrobianos de aislamientos clínicos de Staphylococcus spp. resistentes a meticilina y Enterococcus spp.
Saved in:
| Title: | Estudio comparativo de Wider, E-test y microdilución para la determinación de la sensibilidad a daptomicina y otros tres antimicrobianos de aislamientos clínicos de Staphylococcus spp. resistentes a meticilina y Enterococcus spp. |
|---|---|
| Alternate Title: | Comparative study of the susceptibility to daptomycin and other antimicrobials against Staphylococcus spp. resistant to methicillin and Enterococcus spp. using Wider, E-test, and microdilution methods. |
| Authors: | Gómez-Garcés, José Luis1 jlgarces@microb.net, López-Fabal, Fátima1, Burillo, Almudena1, Gil, Yolanda1 |
| Source: | Revista Española de Quimioterapia. jun2010, Vol. 23 Issue 2, p87-92. 6p. 3 Charts. |
| Subjects: | DISEASE susceptibility, ANTI-infective agents, STAPHYLOCOCCUS, METHICILLIN resistance, ENTEROCOCCUS, COMPARATIVE studies |
| Abstract (English): | The human and material costs of inappropriate antimicrobial therapy are high. This study was designed to search for a rapid, simple and effective antimicrobial susceptibility test capable of identifying the best treatment strategy against microorganisms causing hospital infections showing resistance or reduced susceptibility to the more traditional antibiotics. The tests compared were the E-test, an automated test (Wider) and broth microdilution (as the reference test), to determine the susceptibility to vancomycin, teicoplanin, linezolid and daptomycin of clinical isolates of methicillin resistant Staphylococcus aureus (MRSA), methicillin resistant coagulase negative Staphylococcus spp. and Enterococccus spp. The E-test and Wider methods showed good agreement with the reference method indicating their reliability for routine susceptibility testing of staphylococci and enterococci against vancomycin, teicoplanin, linezolid and daptomycin. Notwithstanding, when faced with a serious enterococcal infection, the MIC of daptomycin should be more accurately determined using a reference technique such as broth microdilution. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Los costes humanos y materiales del tratamiento antimicrobiano inadecuado son altos. El objetivo de este trabajo es valorar métodos de determinación de la sensibilidad antimicrobiana rápidos, sencillos y eficientes que permitan identificar la mejor opción terapéutica en el caso de infecciones hospitalarias producidas por microorganismos resistentes o con sensibilidad disminuida frente a antibióticos tradicionales. Los métodos comparados han sido el gradiente de difusión (Etest), un método automatizado (Wider) y la microdilución, este último considerado de referencia, para vancomicina, teicoplanina, linezolid y daptomicina frente a aislamientos clínicos de Staphylococcus aureus resistentes a meticilina (SARM), Staphylococcus coagulasa negativo resistentes a meticilina (SCNRM) y Enterococccus spp. Tanto E-test como Wider resultan métodos fiables para su uso en la rutina microbiológica a la hora de categorizar la sensibilidad de estafilococos y enterococos frente a vancomicina, teicoplanina, linezolid y daptomicina. Solamente en aquellos casos de infecciones graves por enterococos, que requieran la determinación de la CMI de daptomicina con exactitud, es recomendable emplear un técnica de referencia como es la microdilución. [ABSTRACT FROM AUTHOR] |
| Copyright of Revista Española de Quimioterapia is the property of Sociedad Espanola de Quimioterapia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
|---|---|
| Header | DbId: lth DbLabel: MedicLatina An: 52248701 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Estudio comparativo de Wider, E-test y microdilución para la determinación de la sensibilidad a daptomicina y otros tres antimicrobianos de aislamientos clínicos de Staphylococcus spp. resistentes a meticilina y Enterococcus spp. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: Comparative study of the susceptibility to daptomycin and other antimicrobials against Staphylococcus spp. resistant to methicillin and Enterococcus spp. using Wider, E-test, and microdilution methods. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Gómez-Garcés%2C+José+Luis%22">Gómez-Garcés, José Luis</searchLink><relatesTo>1</relatesTo><i> jlgarces@microb.net</i><br /><searchLink fieldCode="AR" term="%22López-Fabal%2C+Fátima%22">López-Fabal, Fátima</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Burillo%2C+Almudena%22">Burillo, Almudena</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Gil%2C+Yolanda%22">Gil, Yolanda</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Revista+Española+de+Quimioterapia%22">Revista Española de Quimioterapia</searchLink>. jun2010, Vol. 23 Issue 2, p87-92. 6p. 3 Charts. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22DISEASE+susceptibility%22">DISEASE susceptibility</searchLink><br /><searchLink fieldCode="DE" term="%22ANTI-infective+agents%22">ANTI-infective agents</searchLink><br /><searchLink fieldCode="DE" term="%22STAPHYLOCOCCUS%22">STAPHYLOCOCCUS</searchLink><br /><searchLink fieldCode="DE" term="%22METHICILLIN+resistance%22">METHICILLIN resistance</searchLink><br /><searchLink fieldCode="DE" term="%22ENTEROCOCCUS%22">ENTEROCOCCUS</searchLink><br /><searchLink fieldCode="DE" term="%22COMPARATIVE+studies%22">COMPARATIVE studies</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: The human and material costs of inappropriate antimicrobial therapy are high. This study was designed to search for a rapid, simple and effective antimicrobial susceptibility test capable of identifying the best treatment strategy against microorganisms causing hospital infections showing resistance or reduced susceptibility to the more traditional antibiotics. The tests compared were the E-test, an automated test (Wider) and broth microdilution (as the reference test), to determine the susceptibility to vancomycin, teicoplanin, linezolid and daptomycin of clinical isolates of methicillin resistant Staphylococcus aureus (MRSA), methicillin resistant coagulase negative Staphylococcus spp. and Enterococccus spp. The E-test and Wider methods showed good agreement with the reference method indicating their reliability for routine susceptibility testing of staphylococci and enterococci against vancomycin, teicoplanin, linezolid and daptomycin. Notwithstanding, when faced with a serious enterococcal infection, the MIC of daptomycin should be more accurately determined using a reference technique such as broth microdilution. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Los costes humanos y materiales del tratamiento antimicrobiano inadecuado son altos. El objetivo de este trabajo es valorar métodos de determinación de la sensibilidad antimicrobiana rápidos, sencillos y eficientes que permitan identificar la mejor opción terapéutica en el caso de infecciones hospitalarias producidas por microorganismos resistentes o con sensibilidad disminuida frente a antibióticos tradicionales. Los métodos comparados han sido el gradiente de difusión (Etest), un método automatizado (Wider) y la microdilución, este último considerado de referencia, para vancomicina, teicoplanina, linezolid y daptomicina frente a aislamientos clínicos de Staphylococcus aureus resistentes a meticilina (SARM), Staphylococcus coagulasa negativo resistentes a meticilina (SCNRM) y Enterococccus spp. Tanto E-test como Wider resultan métodos fiables para su uso en la rutina microbiológica a la hora de categorizar la sensibilidad de estafilococos y enterococos frente a vancomicina, teicoplanina, linezolid y daptomicina. Solamente en aquellos casos de infecciones graves por enterococos, que requieran la determinación de la CMI de daptomicina con exactitud, es recomendable emplear un técnica de referencia como es la microdilución. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Revista Española de Quimioterapia is the property of Sociedad Espanola de Quimioterapia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lth&AN=52248701 |
| RecordInfo | BibRecord: BibEntity: Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 6 StartPage: 87 Subjects: – SubjectFull: DISEASE susceptibility Type: general – SubjectFull: ANTI-infective agents Type: general – SubjectFull: STAPHYLOCOCCUS Type: general – SubjectFull: METHICILLIN resistance Type: general – SubjectFull: ENTEROCOCCUS Type: general – SubjectFull: COMPARATIVE studies Type: general Titles: – TitleFull: Estudio comparativo de Wider, E-test y microdilución para la determinación de la sensibilidad a daptomicina y otros tres antimicrobianos de aislamientos clínicos de Staphylococcus spp. resistentes a meticilina y Enterococcus spp. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Gómez-Garcés, José Luis – PersonEntity: Name: NameFull: López-Fabal, Fátima – PersonEntity: Name: NameFull: Burillo, Almudena – PersonEntity: Name: NameFull: Gil, Yolanda IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: jun2010 Type: published Y: 2010 Identifiers: – Type: issn-print Value: 02143429 Numbering: – Type: volume Value: 23 – Type: issue Value: 2 Titles: – TitleFull: Revista Española de Quimioterapia Type: main |
| ResultId | 1 |
