Bibliographic Details
| Title: |
Epidemiologia genomica y paraparesia espstica tropical asociada a la infeccion por el virus linfotropico humano de clulas T tipo 1. |
| Alternate Title: |
Genome epidemiology and tropical spastic paraparesis associated with human T-cell lymphotropic virus type 1. |
| Authors: |
Salcedo-Cifuentes, Mercedes1, Dominguez, Martha C.1, Garcia-Vallejo, Felipe1 labiomol@hotmail.com |
| Source: |
Pan American Journal of Public Health / Revista Panamericana de Salud Pública. Nov2011, Vol. 30 Issue 5, p422-430. 9p. |
| Subjects: |
GENE mapping, GENETIC techniques, HIV, HUMAN genome, LYMPHOCYTES, PUBLIC health surveillance, SPINAL cord diseases, DISEASE prevalence, MYELITIS, DATA analysis software, GENETICS |
| Geographic Terms: |
COLOMBIA, JAPAN |
| Abstract (English): |
Objective. Characterize the genomic environment of the sequences adjacent to human T-cell lymphotropic virus type 1 (HTLV-1) in patients with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) in different regions of Colombia and Japan. Methods. A total of 71 recombinant clones with human genome sequences adjacent to 5' LTR in patients with HAM/TSP were compared to the Genome Browser and GenBank databases. Sixteen structural and compositional genome variables were identified, and statistical analysis was conducted in the R computer program, version 2.8.1, in a 0.5 Mb window. Results. A total of 43.0% of the proviruses were located in the group C chromosomes; 74% of the sequences were located in the telomeric and subtelomeric regions (P < 0.05). A cluster analysis was used to establish the hierarchical relations between the genome characteristics included in the study. The analysis of principal components identified the components that defined the preferred genome environments for proviral integration in cases of HAM/TSP. Conclusions. HTLV-1 was integrated more often in chromatin regions rich in CpG islands with a high density of genes and LINE type repetitions, and DNA transposons which, overall, would form the genomic environments targeted for integration. This new scenario will promote substantial changes in the field of public health and in epidemiological management of infectious diseases. It will also foster the development of powerful tools for increasing the efficiency of epidemiological surveillance. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): |
Objetivo. Caracterizar el ambiente genómico de las secuencias adyacentes al virus linfotrópico humano de células T tipo 1 (HTLV-1) en pacientes con paraparesia espástica tropical y mielopatía asociada a la infección con HTLV-1 (PET/MAH) de diferentes regiones de Colombia y del Japón. Métodos. Se enfrentaron 71 clones recombinantes con secuencias del genoma humano adyacentes al 5′-LTR de pacientes con PET/MAH, a las bases de datos del Genome Browser y del Gen- Bank. Se identificaron y analizaron estadísticamente 16 variables genómicas estructurales y composicionales mediante el programa informático R, versión 2.8.1, en una ventana de 0,5 Mb. Resultados. El 43,0% de los provirus se localizaron en los cromosomas del grupo C; 74% de las secuencias se ubicaron en regiones teloméricas y subteloméricas (P < 0,05). Un análisis de conglomerados permitió establecer las relaciones jerárquicas entre las características genómicas incluidas en el estudio; el análisis de componentes principales identificó las componentes que definieron los ambientes genómicos preferidos para la integración proviral en casos de PET/MAH. Conclusiones. El HTLV-1 se integró con mayor frecuencia en regiones de la cromatina ricas en islas de citocina fosfato guanina (CpG), de alta densidad de genes y de repeticiones tipo LINE (elemento disperso largo [long interspersed element]) y transposones de ADN que, en conjunto, conformarían los ambientes genómicos blanco de integración. Este nuevo escenario promoverá cambios sustanciales en el campo de la salud pública y en el manejo epidemiológico de las enfermedades infecciosas, y permitirá desarrollar potentes herramientas para incrementar la eficiencia de la vigilancia epidemiológica [ABSTRACT FROM AUTHOR] |
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| Database: |
MedicLatina |
