Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela.
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| Title: | Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela. |
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| Alternate Title: | Phenotypic and genotypic characterization of twelve rhizobial isolates from different regions of Venezuela. |
| Authors: | Marquina, María Eugenia1 martu@ula.ve, González, Néstor Enrique1 ngonzalezv2007@gmail.com, Castro, Yulimar1 yulica@gmail.com |
| Source: | Revista de Biología Tropical. sep2011, Vol. 59 Issue 3, p1017-1036. 20p. |
| Subjects: | SOIL microbiology, PHENOTYPES, BIOLOGICAL variation, BACTERIAL genetics, BIOLOGICAL classification, GENETIC markers, DNA |
| Geographic Terms: | VENEZUELA |
| Abstract (English): | Rhizobial taxonomy and systematics have progressed substantially, nevertheless, few studies have been developed on venezuelan species. This study evaluated the phenotypic and genetic variation between 12 venezuelan indigenous rhizobial isolates and 10 international referential strains, by phenotypical traits and DNA molecular markers. in this regard, a PCR-RFLP of the 16S rDNA gene, the presence of large plasmids, metabolic assays in solid media, salinity resistance, pH and temperature growth conditions, and intrinsic antibiotic resistance were assayed. in reference to the phenotypic attributes, we recognized three main groups: A group i, which comprised all the strains metabolizing between 67.5%-90% of the C and N sources. They were also acid-tolerant, as well as acid producers, capable of growing at 40°C and in high salinity conditions (2-2.5% NaC1). With regard to the antibiotic sensitivity, this group was susceptible to a 30% of the antibiotic assayed. Strains belonging to Group ii exhibited a lower salt tolerance (0.1-1.5%NaCl), as well as a lower acid tolerance, since they grew well at pH values equal or higher than 5.0. This group appeared to be resistant to all of the antibiotics assayed and only metabolized between 52.5%-82.5% of the C and N sources. Group iii was represented by a single bacterial strain: it has a extremely low salt tolerance (0.1% NaC1). This strain grew at a pH equal or higher than 5.6, was susceptible to 50% of the antibiotics assayed and metabolized 72% of the C and N sources. On the basis of a PCR- RFLP of the 16S rDNA, three groups were also obtained. Members of the group A showed a close resemblance to Rhizobium tropici CiAT 899 and Sinorhizobium americanum CFN-Ei 156, while Group B was closely related to Bradyrhizobium spp. Group C, was also represented by only one isolate. The Trebol isolate, was the only one strain able to form nodules and does not appear to be related to any of the referential rhizobial strains, suggesting a possible symbiotic horizontal gene transfer. Finally, in this work, there are evidences of a genetic diversity in the venezuelan rhizobial strains. A different geographical origin is perhaps an important factor affecting the diversity of the indigenous rhizobia in this study. Rev. Biol. Trop. 59 (3): 1017-1036. Epub 2011 September 01. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Rasgos fenotípicos y marcadores moleculares de ADN se utilizaron para investigar la variación fenotípica y genética entre 12 aislados rizobianos venezolanos y 10 cepas de referencia internacionales. Para ello, se realizó un PCR-RFLP del gen rDNA 16S, la presencia de plásmidos grandes, análisis metabólicos en medios sólidos, resistencia a la salinidad, condiciones del crecimiento a diferentes pH y temperaturas y la resistencia intrínseca a antibióticos. En referencia a las cualidades fenotípicas, se diferenciaron tres grupos principales, un grupo i que abarcó a todas aquellas cepas que metabolizaban entre 67.5% y 90% de las fuentes de C y de N. También eran tolerantes a la acidez y productoras de ácido, capaces de crecer a 40ºC y a altas condiciones de salinidad (NaCl 2-2.5%). Con respecto a la sensibilidad a antibióticos, este grupo era susceptible a un 30% de los antibióticos empleados. Las cepas que pertenecen al grupo ii exhibieron una tolerancia salina más baja (0.1-1.5%NaCl), así como una menor tolerancia a la acidez, puesto que crecieron bien en valores de pH iguales o mayores a 5.0. Este grupo era resistente a todos los antibióticos probados y metabolizaban solamente entre 52.5%- 82.5% de las fuentes de C y de N. Una sola cepa bacteriana representó al grupo iii, con una baja tolerancia salina (0.1% NaCl). Este aislado creció a un pH mayor o igual a 5.6, era susceptible a 50% de los antibióticos probados y metabolizó el 72% de las fuentes de C y de N. Al tener como base el PCR-RFLP del 16S rDNA, se diferenciaron también tres grupos. Los miembros del grupo A demostraron una estrecha relación con Rhizobium tropici CiAT 899 y Sinorhizobium americanum CFN-Ei156, mientras que el grupo B está estrechamente vinculado a Bradyrhizobium spp. El grupo C, está representado por solo un aislado. El aislado Trebol, fue la única cepa capaz de formar nódulos y no aparece relacionado con ninguna cepa de referencia, y sugiere una transferencia horizontal de genes simbióticos. Finalmente, en este trabajo se evidencia una diversidad genética en las cepas rizobianas venezolanas. El origen geográfico diverso de estas cepas, quizás sea un factor importante que influencie la diversidad de los rizobios indígenas utilizados en este estudio. [ABSTRACT FROM AUTHOR] |
| Copyright of Revista de Biología Tropical is the property of Universidad de Costa Rica and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Items | – Name: Title Label: Title Group: Ti Data: Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: Phenotypic and genotypic characterization of twelve rhizobial isolates from different regions of Venezuela. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Marquina%2C+María+Eugenia%22">Marquina, María Eugenia</searchLink><relatesTo>1</relatesTo><i> martu@ula.ve</i><br /><searchLink fieldCode="AR" term="%22González%2C+Néstor+Enrique%22">González, Néstor Enrique</searchLink><relatesTo>1</relatesTo><i> ngonzalezv2007@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Castro%2C+Yulimar%22">Castro, Yulimar</searchLink><relatesTo>1</relatesTo><i> yulica@gmail.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Revista+de+Biología+Tropical%22">Revista de Biología Tropical</searchLink>. sep2011, Vol. 59 Issue 3, p1017-1036. 20p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22SOIL+microbiology%22">SOIL microbiology</searchLink><br /><searchLink fieldCode="DE" term="%22PHENOTYPES%22">PHENOTYPES</searchLink><br /><searchLink fieldCode="DE" term="%22BIOLOGICAL+variation%22">BIOLOGICAL variation</searchLink><br /><searchLink fieldCode="DE" term="%22BACTERIAL+genetics%22">BACTERIAL genetics</searchLink><br /><searchLink fieldCode="DE" term="%22BIOLOGICAL+classification%22">BIOLOGICAL classification</searchLink><br /><searchLink fieldCode="DE" term="%22GENETIC+markers%22">GENETIC markers</searchLink><br /><searchLink fieldCode="DE" term="%22DNA%22">DNA</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22VENEZUELA%22">VENEZUELA</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: Rhizobial taxonomy and systematics have progressed substantially, nevertheless, few studies have been developed on venezuelan species. This study evaluated the phenotypic and genetic variation between 12 venezuelan indigenous rhizobial isolates and 10 international referential strains, by phenotypical traits and DNA molecular markers. in this regard, a PCR-RFLP of the 16S rDNA gene, the presence of large plasmids, metabolic assays in solid media, salinity resistance, pH and temperature growth conditions, and intrinsic antibiotic resistance were assayed. in reference to the phenotypic attributes, we recognized three main groups: A group i, which comprised all the strains metabolizing between 67.5%-90% of the C and N sources. They were also acid-tolerant, as well as acid producers, capable of growing at 40°C and in high salinity conditions (2-2.5% NaC1). With regard to the antibiotic sensitivity, this group was susceptible to a 30% of the antibiotic assayed. Strains belonging to Group ii exhibited a lower salt tolerance (0.1-1.5%NaCl), as well as a lower acid tolerance, since they grew well at pH values equal or higher than 5.0. This group appeared to be resistant to all of the antibiotics assayed and only metabolized between 52.5%-82.5% of the C and N sources. Group iii was represented by a single bacterial strain: it has a extremely low salt tolerance (0.1% NaC1). This strain grew at a pH equal or higher than 5.6, was susceptible to 50% of the antibiotics assayed and metabolized 72% of the C and N sources. On the basis of a PCR- RFLP of the 16S rDNA, three groups were also obtained. Members of the group A showed a close resemblance to Rhizobium tropici CiAT 899 and Sinorhizobium americanum CFN-Ei 156, while Group B was closely related to Bradyrhizobium spp. Group C, was also represented by only one isolate. The Trebol isolate, was the only one strain able to form nodules and does not appear to be related to any of the referential rhizobial strains, suggesting a possible symbiotic horizontal gene transfer. Finally, in this work, there are evidences of a genetic diversity in the venezuelan rhizobial strains. A different geographical origin is perhaps an important factor affecting the diversity of the indigenous rhizobia in this study. Rev. Biol. Trop. 59 (3): 1017-1036. Epub 2011 September 01. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Rasgos fenotípicos y marcadores moleculares de ADN se utilizaron para investigar la variación fenotípica y genética entre 12 aislados rizobianos venezolanos y 10 cepas de referencia internacionales. Para ello, se realizó un PCR-RFLP del gen rDNA 16S, la presencia de plásmidos grandes, análisis metabólicos en medios sólidos, resistencia a la salinidad, condiciones del crecimiento a diferentes pH y temperaturas y la resistencia intrínseca a antibióticos. En referencia a las cualidades fenotípicas, se diferenciaron tres grupos principales, un grupo i que abarcó a todas aquellas cepas que metabolizaban entre 67.5% y 90% de las fuentes de C y de N. También eran tolerantes a la acidez y productoras de ácido, capaces de crecer a 40ºC y a altas condiciones de salinidad (NaCl 2-2.5%). Con respecto a la sensibilidad a antibióticos, este grupo era susceptible a un 30% de los antibióticos empleados. Las cepas que pertenecen al grupo ii exhibieron una tolerancia salina más baja (0.1-1.5%NaCl), así como una menor tolerancia a la acidez, puesto que crecieron bien en valores de pH iguales o mayores a 5.0. Este grupo era resistente a todos los antibióticos probados y metabolizaban solamente entre 52.5%- 82.5% de las fuentes de C y de N. Una sola cepa bacteriana representó al grupo iii, con una baja tolerancia salina (0.1% NaCl). Este aislado creció a un pH mayor o igual a 5.6, era susceptible a 50% de los antibióticos probados y metabolizó el 72% de las fuentes de C y de N. Al tener como base el PCR-RFLP del 16S rDNA, se diferenciaron también tres grupos. Los miembros del grupo A demostraron una estrecha relación con Rhizobium tropici CiAT 899 y Sinorhizobium americanum CFN-Ei156, mientras que el grupo B está estrechamente vinculado a Bradyrhizobium spp. El grupo C, está representado por solo un aislado. El aislado Trebol, fue la única cepa capaz de formar nódulos y no aparece relacionado con ninguna cepa de referencia, y sugiere una transferencia horizontal de genes simbióticos. Finalmente, en este trabajo se evidencia una diversidad genética en las cepas rizobianas venezolanas. El origen geográfico diverso de estas cepas, quizás sea un factor importante que influencie la diversidad de los rizobios indígenas utilizados en este estudio. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Revista de Biología Tropical is the property of Universidad de Costa Rica and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 20 StartPage: 1017 Subjects: – SubjectFull: SOIL microbiology Type: general – SubjectFull: PHENOTYPES Type: general – SubjectFull: BIOLOGICAL variation Type: general – SubjectFull: BACTERIAL genetics Type: general – SubjectFull: BIOLOGICAL classification Type: general – SubjectFull: GENETIC markers Type: general – SubjectFull: DNA Type: general – SubjectFull: VENEZUELA Type: general Titles: – TitleFull: Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Marquina, María Eugenia – PersonEntity: Name: NameFull: González, Néstor Enrique – PersonEntity: Name: NameFull: Castro, Yulimar IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: sep2011 Type: published Y: 2011 Identifiers: – Type: issn-print Value: 00347744 Numbering: – Type: volume Value: 59 – Type: issue Value: 3 Titles: – TitleFull: Revista de Biología Tropical Type: main |
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