Ontogenia de los estróbilos, desarrollo de los esporangios y esporogénesis de Equisetum giganteum (Equisetaceae) en los Andes de Colombia.
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| Title: | Ontogenia de los estróbilos, desarrollo de los esporangios y esporogénesis de Equisetum giganteum (Equisetaceae) en los Andes de Colombia. |
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| Alternate Title: | Ontogeny of strobili, sporangia development and sporogenesis in Equisetum giganteum (Equisetaceae) from the Colombian Andes. |
| Authors: | Rincón Barón, Edgar Javier1,2 ejrbaron@gmail.com, Forero Ballesteros, Helkin Giovani2,3 helfos85@gmail.com, Gélvez Landazábal, Leidy Viviana2,3 vivigela@gmail.com, Torres, Gerardo Andrés4 gantorres@gmail.com, Rolleri, Cristina Hilda5,6 tinar.cris@gmail.com |
| Source: | Revista de Biología Tropical. dic2011, Vol. 59 Issue 4, p1845-1858. 14p. |
| Subjects: | SPOROGENESIS in plants, ONTOGENY, STROBILIDIIDAE, SPORANGIUM, EQUISETACEAE |
| Geographic Terms: | FRIO River (Costa Rica), ANDES, COLOMBIA, COSTA Rica |
| Abstract (English): | Studies on the ontogeny of the strobilus, sporangium and reproductive biology of this group of ferns are scarce. Here we describe the ontogeny of the strobilus and sporangia, and the process of sporogenesis using specimens of E. giganteum from Colombia collected along the Rio Frio, Distrito de Sevilla, Piedecuesta, Santander, at 2 200m altitude. The strobili in different stages of development were fixed, dehydrated, embedded in paraffin, sectioned using a rotatory microtome and stained with the safranin O and fast green technique. Observations were made using differential interference contrast microscopy (DIC) or Nomarski microscopy, an optical microscopy illumination technique that enhances the contrast in unstained, transparent. Strobili arise and begin to develop in the apical meristems of the main axis and lateral branches, with no significant differences in the ontogeny of strobili of one or other axis. Successive processes of cell division and differentiation lead to the growth of the strobilus and the formation of sporangiophores. These are formed by the scutellum, the manubrium or pedicel-like, basal part of the sporangiophore, and initial cells of sporangium, which differentiate to form the sporangium wall, the sporocytes and the tapetum. There is not formation of a characteristic arquesporium, as sporocytes quickly undergo meiosis originating tetrads of spores. The tapetum retains its histological integrity, but subsequently the cell walls break down and form a plasmodium that invades the sporangial cavity, partially surrounding the tetrads, and then the spores. Towards the end of the sporogenesis the tapetum disintegrates leaving spores with elaters free within the sporangial cavity. Two layers finally form the sporangium wall: the sporangium wall itself, with thickened, lignified cell walls and an underlying pyknotic layer. The mature spores are chlorofilous, morphologically similar and have exospore, a thin perispore and two elaters. This study of the ontogeny of the spore-producing structures and spores is the first contribution of this type for a tropical species of the genus. Fluorescence microscopy indicates that elaters and the wall of the sporangium are autofluorescent, while other structures induced fluorescence emitted by the fluorescent dye safranin O. The results were also discussed in relation to what is known so far for other species of Equisetum, suggesting that ontogenetic processes and structure of characters sporoderm are relatively constant in Equisetum, which implies important diagnostic value in the taxonomy of the group. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Estudios sobre la ontogenia del estróbilo, los esporangios y la biología reproductiva de Equisetum son escasos, por lo tanto, para la especie E. giganteum, se estudiaron estos aspectos en especímenes recolectados a orillas del Río Frío, Santander, Colombia (2 200m). Los estróbilos en diferentes etapas de maduración fueron fijados, deshidratados, embebidos en parafina, seccionados en micrótomo rotatorio y teñidos con safranina O-fast green. Las observaciones se efectuaron mediante un microscopio óptico de alta resolución con contraste diferencial de interferencia (DIC) y microscopio de fluorescencia. Los estróbilos se inician a partir del meristemo apical, tanto en el eje principal como en los laterales, sin diferencias en el proceso de ontogenia y esporogénesis entre estróbilos de diferentes ejes. Sucesivas mitosis y diferenciación celular conducen al crecimiento del estróbilo, y a la formación de los esporangióforos peltados, formados por el manubrio, o porción basal con aspecto de pedicelo, el escutelo, o porción apical aplanada y las iniciales del esporangio, los cuales se diferenciarán para formar la pared del esporangio, los esporocitos y el tapete. No se forma arquesporio y los esporocitos experimentan meiosis para formar tétradas de esporas. El tapete mantiene la integridad histológica hasta la formación de las tétradas y en esa etapa forma un plasmodio que invade la cavidad esporangial la cual rodea parcialmente las tétradas y luego las esporas, y aparecen las cámaras plasmodiales, un término propuesto aquí para las formaciones designadas en inglés "tapetal gaps". La pared del esporangio queda reducida a dos capas celulares: una externa con engrosamientos lignificados en todas las paredes celulares y una interna picnótica. Al finalizar la esporogénesis, el tapete degenera, y las esporas, con exosporio, perisporio delgado, casi membranáceo y eláteres quedan libres en la cavidad esporangial. El esporodermo, los núcleos y nucléolos presentan fluorescencia roja, inducida por coloración con safranina O, mientras que los eláteres y las células de la pared del esporangio presentan autofluorescencia amarillo-naranja. [ABSTRACT FROM AUTHOR] |
| Copyright of Revista de Biología Tropical is the property of Universidad de Costa Rica and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Items | – Name: Title Label: Title Group: Ti Data: Ontogenia de los estróbilos, desarrollo de los esporangios y esporogénesis de Equisetum giganteum (Equisetaceae) en los Andes de Colombia. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: Ontogeny of strobili, sporangia development and sporogenesis in Equisetum giganteum (Equisetaceae) from the Colombian Andes. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Rincón+Barón%2C+Edgar+Javier%22">Rincón Barón, Edgar Javier</searchLink><relatesTo>1,2</relatesTo><i> ejrbaron@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Forero+Ballesteros%2C+Helkin+Giovani%22">Forero Ballesteros, Helkin Giovani</searchLink><relatesTo>2,3</relatesTo><i> helfos85@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Gélvez+Landazábal%2C+Leidy+Viviana%22">Gélvez Landazábal, Leidy Viviana</searchLink><relatesTo>2,3</relatesTo><i> vivigela@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Torres%2C+Gerardo+Andrés%22">Torres, Gerardo Andrés</searchLink><relatesTo>4</relatesTo><i> gantorres@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Rolleri%2C+Cristina+Hilda%22">Rolleri, Cristina Hilda</searchLink><relatesTo>5,6</relatesTo><i> tinar.cris@gmail.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Revista+de+Biología+Tropical%22">Revista de Biología Tropical</searchLink>. dic2011, Vol. 59 Issue 4, p1845-1858. 14p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22SPOROGENESIS+in+plants%22">SPOROGENESIS in plants</searchLink><br /><searchLink fieldCode="DE" term="%22ONTOGENY%22">ONTOGENY</searchLink><br /><searchLink fieldCode="DE" term="%22STROBILIDIIDAE%22">STROBILIDIIDAE</searchLink><br /><searchLink fieldCode="DE" term="%22SPORANGIUM%22">SPORANGIUM</searchLink><br /><searchLink fieldCode="DE" term="%22EQUISETACEAE%22">EQUISETACEAE</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22FRIO+River+%28Costa+Rica%29%22">FRIO River (Costa Rica)</searchLink><br /><searchLink fieldCode="DE" term="%22ANDES%22">ANDES</searchLink><br /><searchLink fieldCode="DE" term="%22COLOMBIA%22">COLOMBIA</searchLink><br /><searchLink fieldCode="DE" term="%22COSTA+Rica%22">COSTA Rica</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: Studies on the ontogeny of the strobilus, sporangium and reproductive biology of this group of ferns are scarce. Here we describe the ontogeny of the strobilus and sporangia, and the process of sporogenesis using specimens of E. giganteum from Colombia collected along the Rio Frio, Distrito de Sevilla, Piedecuesta, Santander, at 2 200m altitude. The strobili in different stages of development were fixed, dehydrated, embedded in paraffin, sectioned using a rotatory microtome and stained with the safranin O and fast green technique. Observations were made using differential interference contrast microscopy (DIC) or Nomarski microscopy, an optical microscopy illumination technique that enhances the contrast in unstained, transparent. Strobili arise and begin to develop in the apical meristems of the main axis and lateral branches, with no significant differences in the ontogeny of strobili of one or other axis. Successive processes of cell division and differentiation lead to the growth of the strobilus and the formation of sporangiophores. These are formed by the scutellum, the manubrium or pedicel-like, basal part of the sporangiophore, and initial cells of sporangium, which differentiate to form the sporangium wall, the sporocytes and the tapetum. There is not formation of a characteristic arquesporium, as sporocytes quickly undergo meiosis originating tetrads of spores. The tapetum retains its histological integrity, but subsequently the cell walls break down and form a plasmodium that invades the sporangial cavity, partially surrounding the tetrads, and then the spores. Towards the end of the sporogenesis the tapetum disintegrates leaving spores with elaters free within the sporangial cavity. Two layers finally form the sporangium wall: the sporangium wall itself, with thickened, lignified cell walls and an underlying pyknotic layer. The mature spores are chlorofilous, morphologically similar and have exospore, a thin perispore and two elaters. This study of the ontogeny of the spore-producing structures and spores is the first contribution of this type for a tropical species of the genus. Fluorescence microscopy indicates that elaters and the wall of the sporangium are autofluorescent, while other structures induced fluorescence emitted by the fluorescent dye safranin O. The results were also discussed in relation to what is known so far for other species of Equisetum, suggesting that ontogenetic processes and structure of characters sporoderm are relatively constant in Equisetum, which implies important diagnostic value in the taxonomy of the group. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Estudios sobre la ontogenia del estróbilo, los esporangios y la biología reproductiva de Equisetum son escasos, por lo tanto, para la especie E. giganteum, se estudiaron estos aspectos en especímenes recolectados a orillas del Río Frío, Santander, Colombia (2 200m). Los estróbilos en diferentes etapas de maduración fueron fijados, deshidratados, embebidos en parafina, seccionados en micrótomo rotatorio y teñidos con safranina O-fast green. Las observaciones se efectuaron mediante un microscopio óptico de alta resolución con contraste diferencial de interferencia (DIC) y microscopio de fluorescencia. Los estróbilos se inician a partir del meristemo apical, tanto en el eje principal como en los laterales, sin diferencias en el proceso de ontogenia y esporogénesis entre estróbilos de diferentes ejes. Sucesivas mitosis y diferenciación celular conducen al crecimiento del estróbilo, y a la formación de los esporangióforos peltados, formados por el manubrio, o porción basal con aspecto de pedicelo, el escutelo, o porción apical aplanada y las iniciales del esporangio, los cuales se diferenciarán para formar la pared del esporangio, los esporocitos y el tapete. No se forma arquesporio y los esporocitos experimentan meiosis para formar tétradas de esporas. El tapete mantiene la integridad histológica hasta la formación de las tétradas y en esa etapa forma un plasmodio que invade la cavidad esporangial la cual rodea parcialmente las tétradas y luego las esporas, y aparecen las cámaras plasmodiales, un término propuesto aquí para las formaciones designadas en inglés "tapetal gaps". La pared del esporangio queda reducida a dos capas celulares: una externa con engrosamientos lignificados en todas las paredes celulares y una interna picnótica. Al finalizar la esporogénesis, el tapete degenera, y las esporas, con exosporio, perisporio delgado, casi membranáceo y eláteres quedan libres en la cavidad esporangial. El esporodermo, los núcleos y nucléolos presentan fluorescencia roja, inducida por coloración con safranina O, mientras que los eláteres y las células de la pared del esporangio presentan autofluorescencia amarillo-naranja. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Revista de Biología Tropical is the property of Universidad de Costa Rica and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 14 StartPage: 1845 Subjects: – SubjectFull: SPOROGENESIS in plants Type: general – SubjectFull: ONTOGENY Type: general – SubjectFull: STROBILIDIIDAE Type: general – SubjectFull: SPORANGIUM Type: general – SubjectFull: EQUISETACEAE Type: general – SubjectFull: FRIO River (Costa Rica) Type: general – SubjectFull: ANDES Type: general – SubjectFull: COLOMBIA Type: general – SubjectFull: COSTA Rica Type: general Titles: – TitleFull: Ontogenia de los estróbilos, desarrollo de los esporangios y esporogénesis de Equisetum giganteum (Equisetaceae) en los Andes de Colombia. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Rincón Barón, Edgar Javier – PersonEntity: Name: NameFull: Forero Ballesteros, Helkin Giovani – PersonEntity: Name: NameFull: Gélvez Landazábal, Leidy Viviana – PersonEntity: Name: NameFull: Torres, Gerardo Andrés – PersonEntity: Name: NameFull: Rolleri, Cristina Hilda IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: dic2011 Type: published Y: 2011 Identifiers: – Type: issn-print Value: 00347744 Numbering: – Type: volume Value: 59 – Type: issue Value: 4 Titles: – TitleFull: Revista de Biología Tropical Type: main |
| ResultId | 1 |
