Identification of messenger RNA of fetoplacental source in maternal plasma of women with normal pregnancies and pregnancies with intrauterine growth restriction.
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| Title: | Identification of messenger RNA of fetoplacental source in maternal plasma of women with normal pregnancies and pregnancies with intrauterine growth restriction. |
|---|---|
| Alternate Title: | Identificacion de RNA mensajero de origen fetoplacentario en plasma materno de mujeres con embarazos normales y gestantes con restriccion de crecimiento intrauterine. |
| Authors: | Ayala Ramírez, Paola1 (AUTHOR) payala@javeriana.edu.co, García Robles, Reggie1 (AUTHOR) rgarcia@javeriana.edu.co, Rojas, Juan Diego2 (AUTHOR) jdiegorojas@yahoo.com, Bermúdez, Martha1,3 (AUTHOR), Bernal, Jaime1 (AUTHOR) jebernal@javeriana.edu.co |
| Source: | Colombia Medica. Jul-Sep2012, Vol. 43 Issue 3, p184-188. 5p. 3 Charts. |
| Subjects: | CORD blood, FETAL growth retardation, GENE expression, POLYMERASE chain reaction, PREGNANCY, PROLACTIN, RNA, U-statistics, REVERSE transcriptase polymerase chain reaction, DATA analysis software, DESCRIPTIVE statistics |
| Geographic Terms: | COLOMBIA |
| Abstract (English): | Objective: to quantify placenta-specific RNA in plasma of women carrying foetuses with intrauterine growth restriction and pregnant women with normal pregnancies. Materials and methods: 8 pregnant women with foetuses with intrauterine growth restriction were studied as well as 18 women with uncomplicated pregnancies in the third pregnancy trimester. Total free RNA was quantified in maternal plasma by spectrophotometry and the gene expression of hPL (Human Placental Lactogen) at the messenger RNA level through technical Real Time-Chain Reaction Polymerase. Results: plasma RNA of fetoplacental origin was successfully detected in 100% of pregnant women. There were no statistically significant differences between the values of total RNA extracted from plasma (p = 0.5975) nor in the messenger RNA expression of hPL gene (p = 0.5785) between cases and controls. Conclusion: messenger RNA of fetoplacental origin can be detected in maternal plasma during pregnancy. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Objetivo: cuantificar RNA específico de placenta en el plasma de mujeres con embarazos con fetos con Restricción de Crecimiento Intrauterino y gestantes con embarazos normales. Materiales y métodos: se estudiaron 8 mujeres con embarazos con fetos con Restricción de Crecimiento Intrauterino y 18 mujeres con embarazos sin complicaciones, en el tercer trimestre de embarazo. Se cuantificó el RNA total libre en plasma materno por espectrofotometría y la expresión del gen hPL (Lactógeno Placentario Humano) a nivel de RNA mensajero por medio de la técnica Reacción en Cadena de la Polimerasa en Tiempo Real. Resultados: se logró detectar RNA en plasma de origen fetoplacentario en el 100% de las gestantes. No se encontraron diferencias estadísticamente significativas entre los valores de RNA total extraído de plasma (p=0,5975) ni en la expresión del RNA mensajero del gen hPL (p=0,5785) entre casos y controles. Conclusión: es posible detectar RNA mensajero de origen fetoplacentario en plasma materno durante el embarazo. [ABSTRACT FROM AUTHOR] |
| Copyright of Colombia Medica is the property of Universidad del Valle and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: lth DbLabel: MedicLatina An: 82559019 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Identification of messenger RNA of fetoplacental source in maternal plasma of women with normal pregnancies and pregnancies with intrauterine growth restriction. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: Identificacion de RNA mensajero de origen fetoplacentario en plasma materno de mujeres con embarazos normales y gestantes con restriccion de crecimiento intrauterine. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ayala+Ramírez%2C+Paola%22">Ayala Ramírez, Paola</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> payala@javeriana.edu.co</i><br /><searchLink fieldCode="AR" term="%22García+Robles%2C+Reggie%22">García Robles, Reggie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> rgarcia@javeriana.edu.co</i><br /><searchLink fieldCode="AR" term="%22Rojas%2C+Juan+Diego%22">Rojas, Juan Diego</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> jdiegorojas@yahoo.com</i><br /><searchLink fieldCode="AR" term="%22Bermúdez%2C+Martha%22">Bermúdez, Martha</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bernal%2C+Jaime%22">Bernal, Jaime</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> jebernal@javeriana.edu.co</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Colombia+Medica%22">Colombia Medica</searchLink>. Jul-Sep2012, Vol. 43 Issue 3, p184-188. 5p. 3 Charts. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22CORD+blood%22">CORD blood</searchLink><br /><searchLink fieldCode="DE" term="%22FETAL+growth+retardation%22">FETAL growth retardation</searchLink><br /><searchLink fieldCode="DE" term="%22GENE+expression%22">GENE expression</searchLink><br /><searchLink fieldCode="DE" term="%22POLYMERASE+chain+reaction%22">POLYMERASE chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22PREGNANCY%22">PREGNANCY</searchLink><br /><searchLink fieldCode="DE" term="%22PROLACTIN%22">PROLACTIN</searchLink><br /><searchLink fieldCode="DE" term="%22RNA%22">RNA</searchLink><br /><searchLink fieldCode="DE" term="%22U-statistics%22">U-statistics</searchLink><br /><searchLink fieldCode="DE" term="%22REVERSE+transcriptase+polymerase+chain+reaction%22">REVERSE transcriptase polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22DATA+analysis+software%22">DATA analysis software</searchLink><br /><searchLink fieldCode="DE" term="%22DESCRIPTIVE+statistics%22">DESCRIPTIVE statistics</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22COLOMBIA%22">COLOMBIA</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: Objective: to quantify placenta-specific RNA in plasma of women carrying foetuses with intrauterine growth restriction and pregnant women with normal pregnancies. Materials and methods: 8 pregnant women with foetuses with intrauterine growth restriction were studied as well as 18 women with uncomplicated pregnancies in the third pregnancy trimester. Total free RNA was quantified in maternal plasma by spectrophotometry and the gene expression of hPL (Human Placental Lactogen) at the messenger RNA level through technical Real Time-Chain Reaction Polymerase. Results: plasma RNA of fetoplacental origin was successfully detected in 100% of pregnant women. There were no statistically significant differences between the values of total RNA extracted from plasma (p = 0.5975) nor in the messenger RNA expression of hPL gene (p = 0.5785) between cases and controls. Conclusion: messenger RNA of fetoplacental origin can be detected in maternal plasma during pregnancy. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Objetivo: cuantificar RNA específico de placenta en el plasma de mujeres con embarazos con fetos con Restricción de Crecimiento Intrauterino y gestantes con embarazos normales. Materiales y métodos: se estudiaron 8 mujeres con embarazos con fetos con Restricción de Crecimiento Intrauterino y 18 mujeres con embarazos sin complicaciones, en el tercer trimestre de embarazo. Se cuantificó el RNA total libre en plasma materno por espectrofotometría y la expresión del gen hPL (Lactógeno Placentario Humano) a nivel de RNA mensajero por medio de la técnica Reacción en Cadena de la Polimerasa en Tiempo Real. Resultados: se logró detectar RNA en plasma de origen fetoplacentario en el 100% de las gestantes. No se encontraron diferencias estadísticamente significativas entre los valores de RNA total extraído de plasma (p=0,5975) ni en la expresión del RNA mensajero del gen hPL (p=0,5785) entre casos y controles. Conclusión: es posible detectar RNA mensajero de origen fetoplacentario en plasma materno durante el embarazo. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Colombia Medica is the property of Universidad del Valle and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.25100/cm.v43i3.863 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 5 StartPage: 184 Subjects: – SubjectFull: CORD blood Type: general – SubjectFull: FETAL growth retardation Type: general – SubjectFull: GENE expression Type: general – SubjectFull: POLYMERASE chain reaction Type: general – SubjectFull: PREGNANCY Type: general – SubjectFull: PROLACTIN Type: general – SubjectFull: RNA Type: general – SubjectFull: U-statistics Type: general – SubjectFull: REVERSE transcriptase polymerase chain reaction Type: general – SubjectFull: DATA analysis software Type: general – SubjectFull: DESCRIPTIVE statistics Type: general – SubjectFull: COLOMBIA Type: general Titles: – TitleFull: Identification of messenger RNA of fetoplacental source in maternal plasma of women with normal pregnancies and pregnancies with intrauterine growth restriction. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ayala Ramírez, Paola – PersonEntity: Name: NameFull: García Robles, Reggie – PersonEntity: Name: NameFull: Rojas, Juan Diego – PersonEntity: Name: NameFull: Bermúdez, Martha – PersonEntity: Name: NameFull: Bernal, Jaime IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: Jul-Sep2012 Type: published Y: 2012 Identifiers: – Type: issn-print Value: 01208322 Numbering: – Type: volume Value: 43 – Type: issue Value: 3 Titles: – TitleFull: Colombia Medica Type: main |
| ResultId | 1 |
