Construcción de matrices como sustitutos dérmicos: aplicación potencial en la regeneración de piel.
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| Title: | Construcción de matrices como sustitutos dérmicos: aplicación potencial en la regeneración de piel. |
|---|---|
| Alternate Title: | MATRIX CONSTRUCTION AS DERMAL SUBSTITUTES: POTENTIAL APPLICATION IN SKIN REGENERATION. |
| Authors: | Valencia Serna, Juliana1, Pineda Molina, Catalina1 cpineda@ces.edu.co |
| Source: | Biomedical Engineering Journal / Revista Ingeniería Biomédica. ene-jun2013, Vol. 7 Issue 13, p10-17. 8p. |
| Subjects: | REGENERATION (Biology), FIBROBLAST growth factors, COLLAGEN, MATRICES (Mathematics), POROSITY, HYALURONIC acid |
| Abstract (English): | Wounds can cause loss of skin that limits the skin regeneration, making the treatment more difficult to address. The tissue engineering has developed skin substitutes that promote skin regeneration; however, it is imperative to find materials that allow fibroblast growth in order to find an appropriate skin substitute. The construction of tridimensional porous collagen and collagenhyaluronic acid matrixes crosslinked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride is presented in order to evaluate their potential use in skin regeneration therapies. Matrix porosity was evaluated. Degradation tests, human fibroblast adherence, viability and proliferation tests were performed. Matrixes had a uniform distributed porosity with mean diameters of 50 μm. Both collagen and collagen-hyaluronic acid (2:8 and 4:6) matrixes presented a progressive degradation rate with similar weight. After 24 hours under culture, the number of fibroblasts seeded on collagen matrixes were doubled, while the number of fibroblast seeded on collagen-hyaluronic acid matrixes remained similar. Even though porous structure and degradation rate of different types of constructed matrixes in this study are similar, collagen matrixes offer better adhesion and proliferation conditions for seeded fibroblasts in comparison with hyaluronic acid added matrixes, making them the best scaffold to be employed as a dermic substitute among the matrixes compared here. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | La pérdida de grandes porciones de piel limita su capacidad de regeneración, haciendo difícil su tratamiento. Mediante la ingeniería de tejidos se han desarrollado sustitutos dérmicos que promueven la regeneración; sin embargo, es necesario hallar un material para el crecimiento de fibroblastos que sea un sustituto dérmico adecuado. En este trabajo se construyeron matrices tridimensionales porosas de colágeno y colágeno-ácido hialurónico evaluando su potencial como sustituto dérmico. Las matrices se entrecruzaron con 1-etil-3-(3-dimetilaminopropil) carbodiimida hidrocloruro. Se evaluó la porosidad, se realizaron pruebas de degradación, evaluación de adherencia celular con fibroblastos humanos y, ensayos de viabilidad y proliferación. Las matrices presentaron poros de 50 μm de diámetro uniformemente distribuidos. Las matrices de colágeno y colágeno-ácido hialurónico (2:8 y 4:6) presentaron una degradación progresiva con pesos similares. Luego de 24 horas en cultivo, los resultados de la prueba de MTT mostraron que los fibroblastos en colágeno proliferaron; mientras que los fibroblastos en colágeno-ácido hialurónico no evidenciaron proliferación. Aunque la estructura porosa y la tasa de degradación de las matrices son similares, las de colágeno brindan mejores condiciones de adhesión y proliferación a los fibroblastos cultivados en comparación con las que tienen ácido hialurónico como adición, convirtiéndose en el mejor andamio para ser empleado como sustituto dérmico entre las matrices evaluadas. [ABSTRACT FROM AUTHOR] |
| Copyright of Biomedical Engineering Journal / Revista Ingeniería Biomédica is the property of Biomedical Engineering Journal / Revista Ingenieria Biomedica and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
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| Header | DbId: lth DbLabel: MedicLatina An: 89920412 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Construcción de matrices como sustitutos dérmicos: aplicación potencial en la regeneración de piel. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: MATRIX CONSTRUCTION AS DERMAL SUBSTITUTES: POTENTIAL APPLICATION IN SKIN REGENERATION. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Valencia+Serna%2C+Juliana%22">Valencia Serna, Juliana</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Pineda+Molina%2C+Catalina%22">Pineda Molina, Catalina</searchLink><relatesTo>1</relatesTo><i> cpineda@ces.edu.co</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biomedical+Engineering+Journal+%2F+Revista+Ingeniería+Biomédica%22">Biomedical Engineering Journal / Revista Ingeniería Biomédica</searchLink>. ene-jun2013, Vol. 7 Issue 13, p10-17. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22REGENERATION+%28Biology%29%22">REGENERATION (Biology)</searchLink><br /><searchLink fieldCode="DE" term="%22FIBROBLAST+growth+factors%22">FIBROBLAST growth factors</searchLink><br /><searchLink fieldCode="DE" term="%22COLLAGEN%22">COLLAGEN</searchLink><br /><searchLink fieldCode="DE" term="%22MATRICES+%28Mathematics%29%22">MATRICES (Mathematics)</searchLink><br /><searchLink fieldCode="DE" term="%22POROSITY%22">POROSITY</searchLink><br /><searchLink fieldCode="DE" term="%22HYALURONIC+acid%22">HYALURONIC acid</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: Wounds can cause loss of skin that limits the skin regeneration, making the treatment more difficult to address. The tissue engineering has developed skin substitutes that promote skin regeneration; however, it is imperative to find materials that allow fibroblast growth in order to find an appropriate skin substitute. The construction of tridimensional porous collagen and collagenhyaluronic acid matrixes crosslinked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride is presented in order to evaluate their potential use in skin regeneration therapies. Matrix porosity was evaluated. Degradation tests, human fibroblast adherence, viability and proliferation tests were performed. Matrixes had a uniform distributed porosity with mean diameters of 50 μm. Both collagen and collagen-hyaluronic acid (2:8 and 4:6) matrixes presented a progressive degradation rate with similar weight. After 24 hours under culture, the number of fibroblasts seeded on collagen matrixes were doubled, while the number of fibroblast seeded on collagen-hyaluronic acid matrixes remained similar. Even though porous structure and degradation rate of different types of constructed matrixes in this study are similar, collagen matrixes offer better adhesion and proliferation conditions for seeded fibroblasts in comparison with hyaluronic acid added matrixes, making them the best scaffold to be employed as a dermic substitute among the matrixes compared here. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: La pérdida de grandes porciones de piel limita su capacidad de regeneración, haciendo difícil su tratamiento. Mediante la ingeniería de tejidos se han desarrollado sustitutos dérmicos que promueven la regeneración; sin embargo, es necesario hallar un material para el crecimiento de fibroblastos que sea un sustituto dérmico adecuado. En este trabajo se construyeron matrices tridimensionales porosas de colágeno y colágeno-ácido hialurónico evaluando su potencial como sustituto dérmico. Las matrices se entrecruzaron con 1-etil-3-(3-dimetilaminopropil) carbodiimida hidrocloruro. Se evaluó la porosidad, se realizaron pruebas de degradación, evaluación de adherencia celular con fibroblastos humanos y, ensayos de viabilidad y proliferación. Las matrices presentaron poros de 50 μm de diámetro uniformemente distribuidos. Las matrices de colágeno y colágeno-ácido hialurónico (2:8 y 4:6) presentaron una degradación progresiva con pesos similares. Luego de 24 horas en cultivo, los resultados de la prueba de MTT mostraron que los fibroblastos en colágeno proliferaron; mientras que los fibroblastos en colágeno-ácido hialurónico no evidenciaron proliferación. Aunque la estructura porosa y la tasa de degradación de las matrices son similares, las de colágeno brindan mejores condiciones de adhesión y proliferación a los fibroblastos cultivados en comparación con las que tienen ácido hialurónico como adición, convirtiéndose en el mejor andamio para ser empleado como sustituto dérmico entre las matrices evaluadas. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biomedical Engineering Journal / Revista Ingeniería Biomédica is the property of Biomedical Engineering Journal / Revista Ingenieria Biomedica and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 8 StartPage: 10 Subjects: – SubjectFull: REGENERATION (Biology) Type: general – SubjectFull: FIBROBLAST growth factors Type: general – SubjectFull: COLLAGEN Type: general – SubjectFull: MATRICES (Mathematics) Type: general – SubjectFull: POROSITY Type: general – SubjectFull: HYALURONIC acid Type: general Titles: – TitleFull: Construcción de matrices como sustitutos dérmicos: aplicación potencial en la regeneración de piel. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Valencia Serna, Juliana – PersonEntity: Name: NameFull: Pineda Molina, Catalina IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: ene-jun2013 Type: published Y: 2013 Identifiers: – Type: issn-print Value: 19099762 Numbering: – Type: volume Value: 7 – Type: issue Value: 13 Titles: – TitleFull: Biomedical Engineering Journal / Revista Ingeniería Biomédica Type: main |
| ResultId | 1 |
