PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins.
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| Title: | PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins. |
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| Authors: | Spitzer J; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA; These authors contributed equally to this work., Hafner M; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA; These authors contributed equally to this work., Landthaler M; Present address: Berlin Institute for Medical Systems Biology, Max-Delbruck-Center for Molecular Medicine, Berlin, Germany., Ascano M; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA., Farazi T; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA., Wardle G; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA., Nusbaum J; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA., Khorshid M; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland., Burger L; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland., Zavolan M; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland., Tuschl T; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA. Electronic address: ttuschl@rockefeller.edu. |
| Source: | Methods in enzymology [Methods Enzymol] 2014; Vol. 539, pp. 113-61. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Journal Info: | Publisher: Academic Press Country of Publication: United States NLM ID: 0212271 Publication Model: Print Cited Medium: Internet ISSN: 1557-7988 (Electronic) Linking ISSN: 00766879 NLM ISO Abbreviation: Methods Enzymol Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
| FullText | Text: Availability: 0 |
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| Header | DbId: mdl DbLabel: MEDLINE Ultimate An: 24581442 AccessLevel: 2 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AU" term="%22Spitzer+J%22">Spitzer J</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA; These authors contributed equally to this work.<br /><searchLink fieldCode="AU" term="%22Hafner+M%22">Hafner M</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA; These authors contributed equally to this work.<br /><searchLink fieldCode="AU" term="%22Landthaler+M%22">Landthaler M</searchLink>; Present address: Berlin Institute for Medical Systems Biology, Max-Delbruck-Center for Molecular Medicine, Berlin, Germany.<br /><searchLink fieldCode="AU" term="%22Ascano+M%22">Ascano M</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Farazi+T%22">Farazi T</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Wardle+G%22">Wardle G</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Nusbaum+J%22">Nusbaum J</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Khorshid+M%22">Khorshid M</searchLink>; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland.<br /><searchLink fieldCode="AU" term="%22Burger+L%22">Burger L</searchLink>; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland.<br /><searchLink fieldCode="AU" term="%22Zavolan+M%22">Zavolan M</searchLink>; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland.<br /><searchLink fieldCode="AU" term="%22Tuschl+T%22">Tuschl T</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA. Electronic address: ttuschl@rockefeller.edu. – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%220212271%22">Methods in enzymology</searchLink> [Methods Enzymol] 2014; Vol. 539, pp. 113-61. – Name: TypePub Label: Publication Type Group: TypPub Data: Journal Article; Research Support, Non-U.S. Gov't – Name: TitleSource Label: Journal Info Group: Src Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Academic+Press%22">Academic Press </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>0212271 <i>Publication Model: </i>Print <i>Cited Medium: </i>Internet <i>ISSN: </i>1557-7988 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2200766879%22">00766879 </searchLink><i>NLM ISO Abbreviation: </i>Methods Enzymol <i>Subsets: </i>MEDLINE |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/B978-0-12-420120-0.00008-6 Languages: – Code: eng Text: English PhysicalDescription: Pagination: StartPage: 113 Titles: – TitleFull: PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Spitzer J – PersonEntity: Name: NameFull: Hafner M – PersonEntity: Name: NameFull: Landthaler M – PersonEntity: Name: NameFull: Ascano M – PersonEntity: Name: NameFull: Farazi T – PersonEntity: Name: NameFull: Wardle G – PersonEntity: Name: NameFull: Nusbaum J – PersonEntity: Name: NameFull: Khorshid M – PersonEntity: Name: NameFull: Burger L – PersonEntity: Name: NameFull: Zavolan M – PersonEntity: Name: NameFull: Tuschl T IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: 2014 Type: published Y: 2014 Identifiers: – Type: issn-electronic Value: 1557-7988 Numbering: – Type: volume Value: 539 Titles: – TitleFull: Methods in enzymology Type: main |
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