PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins.

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Title: PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins.
Authors: Spitzer J; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA; These authors contributed equally to this work., Hafner M; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA; These authors contributed equally to this work., Landthaler M; Present address: Berlin Institute for Medical Systems Biology, Max-Delbruck-Center for Molecular Medicine, Berlin, Germany., Ascano M; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA., Farazi T; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA., Wardle G; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA., Nusbaum J; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA., Khorshid M; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland., Burger L; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland., Zavolan M; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland., Tuschl T; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA. Electronic address: ttuschl@rockefeller.edu.
Source: Methods in enzymology [Methods Enzymol] 2014; Vol. 539, pp. 113-61.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Academic Press Country of Publication: United States NLM ID: 0212271 Publication Model: Print Cited Medium: Internet ISSN: 1557-7988 (Electronic) Linking ISSN: 00766879 NLM ISO Abbreviation: Methods Enzymol Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins.
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  Data: <searchLink fieldCode="AU" term="%22Spitzer+J%22">Spitzer J</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA; These authors contributed equally to this work.<br /><searchLink fieldCode="AU" term="%22Hafner+M%22">Hafner M</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA; These authors contributed equally to this work.<br /><searchLink fieldCode="AU" term="%22Landthaler+M%22">Landthaler M</searchLink>; Present address: Berlin Institute for Medical Systems Biology, Max-Delbruck-Center for Molecular Medicine, Berlin, Germany.<br /><searchLink fieldCode="AU" term="%22Ascano+M%22">Ascano M</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Farazi+T%22">Farazi T</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Wardle+G%22">Wardle G</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Nusbaum+J%22">Nusbaum J</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA.<br /><searchLink fieldCode="AU" term="%22Khorshid+M%22">Khorshid M</searchLink>; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland.<br /><searchLink fieldCode="AU" term="%22Burger+L%22">Burger L</searchLink>; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland.<br /><searchLink fieldCode="AU" term="%22Zavolan+M%22">Zavolan M</searchLink>; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland.<br /><searchLink fieldCode="AU" term="%22Tuschl+T%22">Tuschl T</searchLink>; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA. Electronic address: ttuschl@rockefeller.edu.
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