PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins.
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| Title: | PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins. |
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| Authors: | Spitzer J; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA; These authors contributed equally to this work., Hafner M; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA; These authors contributed equally to this work., Landthaler M; Present address: Berlin Institute for Medical Systems Biology, Max-Delbruck-Center for Molecular Medicine, Berlin, Germany., Ascano M; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA., Farazi T; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA., Wardle G; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA., Nusbaum J; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA., Khorshid M; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland., Burger L; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland., Zavolan M; Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), Basel, Switzerland., Tuschl T; Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, USA. Electronic address: ttuschl@rockefeller.edu. |
| Source: | Methods in enzymology [Methods Enzymol] 2014; Vol. 539, pp. 113-61. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Journal Info: | Publisher: Academic Press Country of Publication: United States NLM ID: 0212271 Publication Model: Print Cited Medium: Internet ISSN: 1557-7988 (Electronic) Linking ISSN: 00766879 NLM ISO Abbreviation: Methods Enzymol Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
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