Impact of DNA extraction, sample dilution, and reagent contamination on 16S rRNA gene sequencing of human feces.
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| Title: | Impact of DNA extraction, sample dilution, and reagent contamination on 16S rRNA gene sequencing of human feces. |
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| Authors: | Velásquez-Mejía EP; Vidarium-Nutrition, Health and Wellness Research Center, Grupo Empresarial Nutresa, Calle 8 sur 50-67, Medellin, 050023, Colombia., de la Cuesta-Zuluaga J; Vidarium-Nutrition, Health and Wellness Research Center, Grupo Empresarial Nutresa, Calle 8 sur 50-67, Medellin, 050023, Colombia.; Max Planck Institute for Developmental Biology, Spemannstraße 35, 72076, Tübingen, Germany., Escobar JS; Vidarium-Nutrition, Health and Wellness Research Center, Grupo Empresarial Nutresa, Calle 8 sur 50-67, Medellin, 050023, Colombia. jsescobar@serviciosnutresa.com. |
| Source: | Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2018 Jan; Vol. 102 (1), pp. 403-411. Date of Electronic Publication: 2017 Oct 27. |
| Publication Type: | Journal Article |
| Journal Info: | Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
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| ISSN: | 1432-0614 |
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| DOI: | 10.1007/s00253-017-8583-z |
