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Impact of DNA extraction, sample dilution, and reagent contamination on 16S rRNA gene sequencing of human feces.

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Title: Impact of DNA extraction, sample dilution, and reagent contamination on 16S rRNA gene sequencing of human feces.
Authors: Velásquez-Mejía EP; Vidarium-Nutrition, Health and Wellness Research Center, Grupo Empresarial Nutresa, Calle 8 sur 50-67, Medellin, 050023, Colombia., de la Cuesta-Zuluaga J; Vidarium-Nutrition, Health and Wellness Research Center, Grupo Empresarial Nutresa, Calle 8 sur 50-67, Medellin, 050023, Colombia.; Max Planck Institute for Developmental Biology, Spemannstraße 35, 72076, Tübingen, Germany., Escobar JS; Vidarium-Nutrition, Health and Wellness Research Center, Grupo Empresarial Nutresa, Calle 8 sur 50-67, Medellin, 050023, Colombia. jsescobar@serviciosnutresa.com.
Source: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2018 Jan; Vol. 102 (1), pp. 403-411. Date of Electronic Publication: 2017 Oct 27.
Publication Type: Journal Article
Journal Info: Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Velásquez-Mejía+EP%22">Velásquez-Mejía EP</searchLink>; Vidarium-Nutrition, Health and Wellness Research Center, Grupo Empresarial Nutresa, Calle 8 sur 50-67, Medellin, 050023, Colombia.<br /><searchLink fieldCode="AU" term="%22de+la+Cuesta-Zuluaga+J%22">de la Cuesta-Zuluaga J</searchLink>; Vidarium-Nutrition, Health and Wellness Research Center, Grupo Empresarial Nutresa, Calle 8 sur 50-67, Medellin, 050023, Colombia.; Max Planck Institute for Developmental Biology, Spemannstraße 35, 72076, Tübingen, Germany.<br /><searchLink fieldCode="AU" term="%22Escobar+JS%22">Escobar JS</searchLink>; Vidarium-Nutrition, Health and Wellness Research Center, Grupo Empresarial Nutresa, Calle 8 sur 50-67, Medellin, 050023, Colombia. jsescobar@serviciosnutresa.com.
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        Value: 10.1007/s00253-017-8583-z
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        Text: English
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