DNA extraction and amplicon production strategies deeply inf luence the outcome of gut mycobiome studies.

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Title: DNA extraction and amplicon production strategies deeply inf luence the outcome of gut mycobiome studies.
Authors: Frau A; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK., Kenny JG; Centre for Genomic Research (CGR), University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK.; Teagasc Food Research Centre, Moorepark, Cork, Ireland., Lenzi L; Centre for Genomic Research (CGR), University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK., Campbell BJ; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK., Ijaz UZ; School of Engineering, University of Glasgow, Oakfield Avenue, Glasgow, G12 8LT, UK., Duckworth CA; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK., Burkitt MD; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK.; Division of Diabetes, Endocrinology and Gastroenterology, University of Manchester, Dover Street, Manchester, M13 9PT, UK., Hall N; Earlham Institute, Colney Ln, Norwich, NR4 7UZ, UK., Anson J; Liverpool Clinical Laboratories Directorate of Infection and Immunity, Royal Liverpool and Broadgreen University Hospitals NHS Trust, Prescot Street, Liverpool, L7 8XP, UK., Darby AC; Centre for Genomic Research (CGR), University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK., Probert CSJ; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK. mdcsjp@liverpool.ac.uk.
Source: Scientific reports [Sci Rep] 2019 Jun 27; Vol. 9 (1), pp. 9328. Date of Electronic Publication: 2019 Jun 27.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
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  Data: <searchLink fieldCode="AU" term="%22Frau+A%22">Frau A</searchLink>; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK.<br /><searchLink fieldCode="AU" term="%22Kenny+JG%22">Kenny JG</searchLink>; Centre for Genomic Research (CGR), University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK.; Teagasc Food Research Centre, Moorepark, Cork, Ireland.<br /><searchLink fieldCode="AU" term="%22Lenzi+L%22">Lenzi L</searchLink>; Centre for Genomic Research (CGR), University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK.<br /><searchLink fieldCode="AU" term="%22Campbell+BJ%22">Campbell BJ</searchLink>; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK.<br /><searchLink fieldCode="AU" term="%22Ijaz+UZ%22">Ijaz UZ</searchLink>; School of Engineering, University of Glasgow, Oakfield Avenue, Glasgow, G12 8LT, UK.<br /><searchLink fieldCode="AU" term="%22Duckworth+CA%22">Duckworth CA</searchLink>; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK.<br /><searchLink fieldCode="AU" term="%22Burkitt+MD%22">Burkitt MD</searchLink>; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK.; Division of Diabetes, Endocrinology and Gastroenterology, University of Manchester, Dover Street, Manchester, M13 9PT, UK.<br /><searchLink fieldCode="AU" term="%22Hall+N%22">Hall N</searchLink>; Earlham Institute, Colney Ln, Norwich, NR4 7UZ, UK.<br /><searchLink fieldCode="AU" term="%22Anson+J%22">Anson J</searchLink>; Liverpool Clinical Laboratories Directorate of Infection and Immunity, Royal Liverpool and Broadgreen University Hospitals NHS Trust, Prescot Street, Liverpool, L7 8XP, UK.<br /><searchLink fieldCode="AU" term="%22Darby+AC%22">Darby AC</searchLink>; Centre for Genomic Research (CGR), University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK.<br /><searchLink fieldCode="AU" term="%22Probert+CSJ%22">Probert CSJ</searchLink>; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK. mdcsjp@liverpool.ac.uk.
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  Data: <searchLink fieldCode="JN" term="%22101563288%22">Scientific reports</searchLink> [Sci Rep] 2019 Jun 27; Vol. 9 (1), pp. 9328. <i>Date of Electronic Publication: </i>2019 Jun 27.
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