DNA extraction and amplicon production strategies deeply inf luence the outcome of gut mycobiome studies.
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| Title: | DNA extraction and amplicon production strategies deeply inf luence the outcome of gut mycobiome studies. |
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| Authors: | Frau A; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK., Kenny JG; Centre for Genomic Research (CGR), University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK.; Teagasc Food Research Centre, Moorepark, Cork, Ireland., Lenzi L; Centre for Genomic Research (CGR), University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK., Campbell BJ; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK., Ijaz UZ; School of Engineering, University of Glasgow, Oakfield Avenue, Glasgow, G12 8LT, UK., Duckworth CA; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK., Burkitt MD; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK.; Division of Diabetes, Endocrinology and Gastroenterology, University of Manchester, Dover Street, Manchester, M13 9PT, UK., Hall N; Earlham Institute, Colney Ln, Norwich, NR4 7UZ, UK., Anson J; Liverpool Clinical Laboratories Directorate of Infection and Immunity, Royal Liverpool and Broadgreen University Hospitals NHS Trust, Prescot Street, Liverpool, L7 8XP, UK., Darby AC; Centre for Genomic Research (CGR), University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK., Probert CSJ; Gastroenterology Research Unit, Department of Cellular & Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Ashton Street, Liverpool, L69 3GE, UK. mdcsjp@liverpool.ac.uk. |
| Source: | Scientific reports [Sci Rep] 2019 Jun 27; Vol. 9 (1), pp. 9328. Date of Electronic Publication: 2019 Jun 27. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Journal Info: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
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| ISSN: | 2045-2322 |
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| DOI: | 10.1038/s41598-019-44974-x |
