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Caution: Plasmid DNA topology affects luciferase assay reproducibility and outcomes.

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Title: Caution: Plasmid DNA topology affects luciferase assay reproducibility and outcomes.
Authors: Tudini E; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.; Department of Genetics & Computational Biology, QIMR Berghofer Medical Research Institute, Herston, 4006 QLD, Australia., Burke LJ; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia., Whiley PJ; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.; Department of Genetics & Computational Biology, QIMR Berghofer Medical Research Institute, Herston, 4006 QLD, Australia., Sevcik J; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.; Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic., Spurdle AB; Department of Genetics & Computational Biology, QIMR Berghofer Medical Research Institute, Herston, 4006 QLD, Australia., Brown MA; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.
Source: BioTechniques [Biotechniques] 2019 Sep; Vol. 67 (3), pp. 94-96. Date of Electronic Publication: 2019 Aug 01.
Publication Type: Letter; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Taylor & Francis Country of Publication: England NLM ID: 8306785 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1940-9818 (Electronic) Linking ISSN: 07366205 NLM ISO Abbreviation: Biotechniques Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1940-9818
DOI:10.2144/btn-2019-0060