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Caution: Plasmid DNA topology affects luciferase assay reproducibility and outcomes.

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Title: Caution: Plasmid DNA topology affects luciferase assay reproducibility and outcomes.
Authors: Tudini E; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.; Department of Genetics & Computational Biology, QIMR Berghofer Medical Research Institute, Herston, 4006 QLD, Australia., Burke LJ; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia., Whiley PJ; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.; Department of Genetics & Computational Biology, QIMR Berghofer Medical Research Institute, Herston, 4006 QLD, Australia., Sevcik J; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.; Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic., Spurdle AB; Department of Genetics & Computational Biology, QIMR Berghofer Medical Research Institute, Herston, 4006 QLD, Australia., Brown MA; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.
Source: BioTechniques [Biotechniques] 2019 Sep; Vol. 67 (3), pp. 94-96. Date of Electronic Publication: 2019 Aug 01.
Publication Type: Letter; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Taylor & Francis Country of Publication: England NLM ID: 8306785 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1940-9818 (Electronic) Linking ISSN: 07366205 NLM ISO Abbreviation: Biotechniques Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Caution: Plasmid DNA topology affects luciferase assay reproducibility and outcomes.
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  Data: <searchLink fieldCode="AU" term="%22Tudini+E%22">Tudini E</searchLink>; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.; Department of Genetics & Computational Biology, QIMR Berghofer Medical Research Institute, Herston, 4006 QLD, Australia.<br /><searchLink fieldCode="AU" term="%22Burke+LJ%22">Burke LJ</searchLink>; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.<br /><searchLink fieldCode="AU" term="%22Whiley+PJ%22">Whiley PJ</searchLink>; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.; Department of Genetics & Computational Biology, QIMR Berghofer Medical Research Institute, Herston, 4006 QLD, Australia.<br /><searchLink fieldCode="AU" term="%22Sevcik+J%22">Sevcik J</searchLink>; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.; Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic.<br /><searchLink fieldCode="AU" term="%22Spurdle+AB%22">Spurdle AB</searchLink>; Department of Genetics & Computational Biology, QIMR Berghofer Medical Research Institute, Herston, 4006 QLD, Australia.<br /><searchLink fieldCode="AU" term="%22Brown+MA%22">Brown MA</searchLink>; School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.
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  Data: <searchLink fieldCode="JN" term="%228306785%22">BioTechniques</searchLink> [Biotechniques] 2019 Sep; Vol. 67 (3), pp. 94-96. <i>Date of Electronic Publication: </i>2019 Aug 01.
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        Value: 10.2144/btn-2019-0060
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