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Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements.

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Title: Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements.
Authors: Fontana J; Molecular Engineering & Sciences Institute, University of Washington, Seattle, 98195, WA, USA., Dong C; Department of Chemistry, University of Washington, Seattle, 98195, WA, USA., Kiattisewee C; Molecular Engineering & Sciences Institute, University of Washington, Seattle, 98195, WA, USA., Chavali VP; Department of Chemical Engineering, University of Washington, Seattle, 98195, WA, USA., Tickman BI; Molecular Engineering & Sciences Institute, University of Washington, Seattle, 98195, WA, USA., Carothers JM; Molecular Engineering & Sciences Institute, University of Washington, Seattle, 98195, WA, USA. jcaroth@uw.edu.; Department of Chemical Engineering, University of Washington, Seattle, 98195, WA, USA. jcaroth@uw.edu.; Center for Synthetic Biology, University of Washington, Seattle, 98195, WA, USA. jcaroth@uw.edu., Zalatan JG; Department of Chemistry, University of Washington, Seattle, 98195, WA, USA. zalatan@uw.edu.; Department of Chemical Engineering, University of Washington, Seattle, 98195, WA, USA. zalatan@uw.edu.; Center for Synthetic Biology, University of Washington, Seattle, 98195, WA, USA. zalatan@uw.edu.
Source: Nature communications [Nat Commun] 2020 Apr 01; Vol. 11 (1), pp. 1618. Date of Electronic Publication: 2020 Apr 01.
Publication Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Fontana+J%22">Fontana J</searchLink>; Molecular Engineering & Sciences Institute, University of Washington, Seattle, 98195, WA, USA.<br /><searchLink fieldCode="AU" term="%22Dong+C%22">Dong C</searchLink>; Department of Chemistry, University of Washington, Seattle, 98195, WA, USA.<br /><searchLink fieldCode="AU" term="%22Kiattisewee+C%22">Kiattisewee C</searchLink>; Molecular Engineering & Sciences Institute, University of Washington, Seattle, 98195, WA, USA.<br /><searchLink fieldCode="AU" term="%22Chavali+VP%22">Chavali VP</searchLink>; Department of Chemical Engineering, University of Washington, Seattle, 98195, WA, USA.<br /><searchLink fieldCode="AU" term="%22Tickman+BI%22">Tickman BI</searchLink>; Molecular Engineering & Sciences Institute, University of Washington, Seattle, 98195, WA, USA.<br /><searchLink fieldCode="AU" term="%22Carothers+JM%22">Carothers JM</searchLink>; Molecular Engineering & Sciences Institute, University of Washington, Seattle, 98195, WA, USA. jcaroth@uw.edu.; Department of Chemical Engineering, University of Washington, Seattle, 98195, WA, USA. jcaroth@uw.edu.; Center for Synthetic Biology, University of Washington, Seattle, 98195, WA, USA. jcaroth@uw.edu.<br /><searchLink fieldCode="AU" term="%22Zalatan+JG%22">Zalatan JG</searchLink>; Department of Chemistry, University of Washington, Seattle, 98195, WA, USA. zalatan@uw.edu.; Department of Chemical Engineering, University of Washington, Seattle, 98195, WA, USA. zalatan@uw.edu.; Center for Synthetic Biology, University of Washington, Seattle, 98195, WA, USA. zalatan@uw.edu.
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  Data: <searchLink fieldCode="JN" term="%22101528555%22">Nature communications</searchLink> [Nat Commun] 2020 Apr 01; Vol. 11 (1), pp. 1618. <i>Date of Electronic Publication: </i>2020 Apr 01.
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